P83489 (OSTCN_BISPR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Osteocalcin

Alternative name(s):
Bone Gla protein
Short name=BGP
Gamma-carboxyglutamic acid-containing protein

Gene names

Name:

BGLAP

Organism

Bison priscus (Steppe wisent) (Steppe bison)

Taxonomic identifier

268291 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bison

Protein attributes

Sequence length	49 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium By similarity. UniProtKB P02820
Subcellular location	Secreted UniProtKB P02820.
Post-translational modification	Gamma-carboxyglutamic acid residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium By similarity. UniProtKB P02820
Miscellaneous	Sequence data obtained by MS from permafrost fossilized bones about 55.6 thousand years old.
Sequence similarities	Belongs to the osteocalcin/matrix Gla protein family. Contains 1 Gla (gamma-carboxy-glutamate) domain.
Mass spectrometry	Molecular mass is 5590 Da from positions 1 - 49. Determined by MALDI. Ref.1

Ontologies

Keywords
Biological process	Biomineralization
Cellular component	Secreted
Ligand	Calcium Metal-binding
PTM	Disulfide bond Gamma-carboxyglutamic acid Hydroxylation
Technical term	Direct protein sequencing Extinct organism protein
Gene Ontology (GO)
Biological_process	biomineral tissue development Inferred from electronic annotation. Source: UniProtKB-KW regulation of bone mineralization Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 49	49	Osteocalcin		PRO_0000148895
Regions
	Domain	1 – 47	47	Gla
Sites
	Metal binding	17	1	Calcium 3 By similarity
	Metal binding	21	1	Calcium 2 By similarity
	Metal binding	24	1	Calcium 1 By similarity
	Metal binding	24	1	Calcium 2 By similarity
	Metal binding	30	1	Calcium 1 By similarity
Amino acid modifications
	Modified residue	9	1	Hydroxyproline UniProtKB P02820
	Modified residue	17	1	4-carboxyglutamate Ref.1 UniProtKB P02820
	Modified residue	21	1	4-carboxyglutamate Ref.1 UniProtKB P02820
	Modified residue	24	1	4-carboxyglutamate Ref.1 UniProtKB P02820
	Disulfide bond	23 ↔ 29		UniProtKB P02820

Sequences

	Sequence		Length	Mass (Da)
	P83489 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 718826015806CCBE	FASTA	49	5,575
	10 20 30 40 YLDHGLGAPA PYPDPLEPKR EVCELNPDCD ELADHIGFQE AYRRFYGPV

References

[1]

"Sequence preservation of osteocalcin protein and mitochondrial DNA in bison bones older than 55 ka." Nielsen-Marsh C.M., Ostrom P.H., Gandhi H., Shapiro B., Cooper A., Hauschka P.V., Collins M.J. Geology 30:1099-1102(2002) Cited for: PROTEIN SEQUENCE, HYDROXYLATION AT PRO-9, GAMMA-CARBOXYGLUTAMATION AT GLU-17; GLU-21 AND GLU-24, MASS SPECTROMETRY. Tissue: Bone.

Web resources

Protein Spotlight

A small blast from the past - Issue 46 of May 2004

Cross-references

3D structure databases
ProteinModelPortal	P83489.
SMR	P83489. Positions 18-49.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000294. GLA_domain. IPR002384. Osteocalcin/MGP. [Graphical view]
Pfam	PF00594. Gla. 1 hit. [Graphical view]
PRINTS	PR00002. GLABONE.
SMART	SM00069. GLA. 1 hit. [Graphical view]
SUPFAM	SSF57630. VitK_dep_GLA. 1 hit.
PROSITE	PS00011. GLA_1. 1 hit. PS50998. GLA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OSTCN_BISPR

Accession

Primary (citable) accession number: P83489

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	March 1, 2003
Last modified:	March 6, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program