Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATP synthase subunit beta-1, mitochondrial

Gene

At5g08670

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi231 – 238ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

ATP synthesis, Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G08670-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP synthase subunit beta-1, mitochondrial (EC:3.6.3.14)
Gene namesi
Ordered Locus Names:At5g08670
ORF Names:T2K12.11
OrganismiArabidopsis thaliana (Mouse-ear cress)Imported
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G08670.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CF(1), Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 51Mitochondrion1 PublicationAdd BLAST51
ChainiPRO_000000243452 – 556ATP synthase subunit beta-1, mitochondrialAdd BLAST505

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei64PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP83483.
PRIDEiP83483.

Interactioni

Subunit structurei

F-type ATPases have 2 components, CF1 - the catalytic core - and CF0 - the membrane proton channel. CF1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. CF0 has three main subunits: a, b and c (By similarity).By similarity

Protein-protein interaction databases

BioGridi16046. 3 interactors.
16048. 2 interactors.
IntActiP83483. 2 interactors.
MINTiMINT-4330205.
STRINGi3702.AT5G08690.1.

Structurei

3D structure databases

ProteinModelPortaliP83483.
SMRiP83483.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ATPase alpha/beta chains family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1350. Eukaryota.
COG0055. LUCA.
HOGENOMiHOG000009605.
InParanoidiP83483.
KOiK02133.
PhylomeDBiP83483.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01347. ATP_synth_beta_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR020971. ATP_synth_F1_beta_su.
IPR005722. ATP_synth_F1_bsu.
IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_F1/V1/A1_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR024034. ATPase_F1/V1_bsu_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
PF11421. Synthase_beta. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01039. atpD. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83483-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASRRVLSSL LRSSSGRSAA KLGNRNPRLP SPSPARHAAP CSYLLGRVAE
60 70 80 90 100
YATSSPASSA APSSAPAKDE GKKTYDYGGK GAIGRVCQVI GAIVDVRFED
110 120 130 140 150
QEGLPPIMTS LEVQDHPTRL VLEVSHHLGQ NVVRTIAMDG TEGLVRGRKV
160 170 180 190 200
LNTGAPITVP VGRATLGRIM NVLGEPIDER GEIKTEHYLP IHRDAPALVD
210 220 230 240 250
LATGQEILAT GIKVVDLLAP YQRGGKIGLF GGAGVGKTVL IMELINNVAK
260 270 280 290 300
AHGGFSVFAG VGERTREGND LYREMIESGV IKLGEKQSES KCALVYGQMN
310 320 330 340 350
EPPGARARVG LTGLTVAEYF RDAEGQDVLL FIDNIFRFTQ ANSEVSALLG
360 370 380 390 400
RIPSAVGYQP TLASDLGALQ ERITTTKKGS ITSVQAIYVP ADDLTDPAPA
410 420 430 440 450
TTFAHLDATT VLSRQISELG IYPAVDPLDS TSRMLSPHIL GEEHYNTARG
460 470 480 490 500
VQKVLQNYKN LQDIIAILGM DELSEDDKLT VARARKIQRF LSQPFHVAEI
510 520 530 540 550
FTGAPGKYVD LKENINSFQG LLDGKYDDLS EQSFYMVGGI DEVVAKAEKI

AKESAA
Length:556
Mass (Da):59,671
Last modified:December 6, 2002 - v1
Checksum:i1BF518B51D93E690
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1M → MTMITPSSNTTHYRESWYAC RYRSGIPGSTHASV in CAC81058 (Ref. 1) Curated1
Sequence conflicti23G → V in BAC43182 (PubMed:11910074).Curated1
Sequence conflicti37H → P in AED91336 (Ref. 3) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271468 mRNA. Translation: CAC81058.1.
AL590346 Genomic DNA. Translation: CAC35872.1.
CP002688 Genomic DNA. Translation: AED91336.1.
AK118538 mRNA. Translation: BAC43141.1.
AK118582 mRNA. Translation: BAC43182.1.
AY054222 mRNA. Translation: AAL06882.1.
AY080681 mRNA. Translation: AAL86357.1.
AY113178 mRNA. Translation: AAM47481.1.
AY117269 mRNA. Translation: AAM51344.1.
RefSeqiNP_568203.2. NM_120953.4.
NP_568204.1. NM_120954.3.
UniGeneiAt.45855.
At.56834.
At.70060.

Genome annotation databases

GeneIDi830768.
830770.
KEGGiath:AT5G08670.
ath:AT5G08690.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271468 mRNA. Translation: CAC81058.1.
AL590346 Genomic DNA. Translation: CAC35872.1.
CP002688 Genomic DNA. Translation: AED91336.1.
AK118538 mRNA. Translation: BAC43141.1.
AK118582 mRNA. Translation: BAC43182.1.
AY054222 mRNA. Translation: AAL06882.1.
AY080681 mRNA. Translation: AAL86357.1.
AY113178 mRNA. Translation: AAM47481.1.
AY117269 mRNA. Translation: AAM51344.1.
RefSeqiNP_568203.2. NM_120953.4.
NP_568204.1. NM_120954.3.
UniGeneiAt.45855.
At.56834.
At.70060.

3D structure databases

ProteinModelPortaliP83483.
SMRiP83483.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi16046. 3 interactors.
16048. 2 interactors.
IntActiP83483. 2 interactors.
MINTiMINT-4330205.
STRINGi3702.AT5G08690.1.

Proteomic databases

PaxDbiP83483.
PRIDEiP83483.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi830768.
830770.
KEGGiath:AT5G08670.
ath:AT5G08690.

Organism-specific databases

TAIRiAT5G08670.

Phylogenomic databases

eggNOGiKOG1350. Eukaryota.
COG0055. LUCA.
HOGENOMiHOG000009605.
InParanoidiP83483.
KOiK02133.
PhylomeDBiP83483.

Enzyme and pathway databases

BioCyciARA:AT5G08670-MONOMER.

Miscellaneous databases

PROiP83483.

Family and domain databases

Gene3Di1.10.1140.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_01347. ATP_synth_beta_bact. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR020971. ATP_synth_F1_beta_su.
IPR005722. ATP_synth_F1_bsu.
IPR020003. ATPase_a/bsu_AS.
IPR004100. ATPase_F1/V1/A1_a/bsu_N.
IPR000194. ATPase_F1/V1/A1_a/bsu_nucl-bd.
IPR024034. ATPase_F1/V1_bsu_C.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00006. ATP-synt_ab. 1 hit.
PF02874. ATP-synt_ab_N. 1 hit.
PF11421. Synthase_beta. 1 hit.
[Graphical view]
SMARTiSM00382. AAA. 1 hit.
[Graphical view]
SUPFAMiSSF50615. SSF50615. 1 hit.
SSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01039. atpD. 1 hit.
PROSITEiPS00152. ATPASE_ALPHA_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiATPBM_ARATH
AccessioniPrimary (citable) accession number: P83483
Secondary accession number(s): F4KCG2
, Q541W2, Q541W7, Q8VX26, Q9C5A8, Q9C5B0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: December 6, 2002
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.