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P83456

- PPB_GADMO

UniProt

P83456 - PPB_GADMO

Protein

Alkaline phosphatase

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 59 (01 Oct 2014)
      Sequence version 1 (15 Dec 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.1 PublicationPROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Temperature dependencei

    Thermolabile.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi44 – 441MagnesiumBy similarity
    Metal bindingi44 – 441Zinc 2By similarity
    Active sitei94 – 941Phosphoserine intermediate
    Metal bindingi155 – 1551MagnesiumBy similarity
    Metal bindingi157 – 1571MagnesiumBy similarity
    Metal bindingi315 – 3151MagnesiumBy similarity
    Metal bindingi320 – 3201Zinc 1By similarity
    Metal bindingi324 – 3241Zinc 1By similarity
    Metal bindingi361 – 3611Zinc 2By similarity
    Metal bindingi362 – 3621Zinc 2By similarity
    Metal bindingi437 – 4371Zinc 1By similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase (EC:3.1.3.1)
    Short name:
    AP
    OrganismiGadus morhua (Atlantic cod)Curated
    Taxonomic identifieri8049 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

    Subcellular locationi

    Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Alkaline phosphatasePRO_0000186166Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi124 – 1241N-linked (GlcNAc...)1 Publication
    Disulfide bondi165 ↔ 1851 Publication
    Glycosylationi214 – 2141N-linked (GlcNAc...)1 Publication
    Glycosylationi413 – 4131N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliP83456.
    SMRiP83456. Positions 3-475.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Phylogenomic databases

    HOVERGENiHBG007345.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P83456-1 [UniParc]FASTAAdd to Basket

    « Hide

    AGFPEQEPEP KFWNDWAQKT LDKALSLQTL NKNKAQNLIL FLGDGMGVPT    50
    VTAARILKGQ LRGQPGEEGQ LEMDKFPFVA LSKTYNTNAQ VADSAGTATA 100
    YLCGVKANEG TVGVSAAAVR SQANTTQGNE VTSILRWAKD AGKSIGIVTT 150
    TRVNHATPSA AYAHCVDRDW YSDNEMPADA VEAGCKDIAR QLFENIPDID 200
    VIMGGGRKYM YPKNTTDVEY PGQPKHSGTR KDGRNLVKEW VDRNTEKKGH 250
    YVWNKKDLLS LNPTKVDYLL GLFEPADLPY DLERNKETDP SLSEMVEVAI 300
    KILRRNPNGF YLLVEGGRID HGHHEGKDKQ AIHEAVEMDR AIGRADLMTS 350
    TSDTLTVVTA DHSHLFSFGG YTPRGNEIFG LAAFISDVDQ KPFTAILYGN 400
    GPGYKLVNGA RENVSTVDYQ DNSYLAQAAV PLSSETHGGE DVAVFAKGPM 450
    AHLLHGVHEQ NYIPHAMAYA ACIGQNR 477
    Length:477
    Mass (Da):52,198
    Last modified:December 15, 2003 - v1
    Checksum:i0637907C62188DF9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence uncertaintyi8 – 81P or K
    Sequence uncertaintyi351 – 3511T or A

    Mass spectrometryi

    Molecular mass is 61000±1100 Da from positions 1 - 477. Determined by MALDI. 1 Publication

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P83456.
    SMRi P83456. Positions 3-475.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG007345.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the cold-active alkaline phosphatase from Atlantic cod (Gadus morhua)."
      Asgeirsson B., Nielsen B.N., Hoejrup P.
      Comp. Biochem. Physiol. 136B:45-60(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY, DISULFIDE BOND, GLYCOSYLATION, 3D-STRUCTURE MODELING.
      Tissue: Small intestine1 Publication.

    Entry informationi

    Entry nameiPPB_GADMO
    AccessioniPrimary (citable) accession number: P83456
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 16, 2004
    Last sequence update: December 15, 2003
    Last modified: October 1, 2014
    This is version 59 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3