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Protein

Alkaline phosphatase

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation1 Publication

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441MagnesiumBy similarity
Metal bindingi44 – 441Zinc 2By similarity
Active sitei94 – 941Phosphoserine intermediate
Metal bindingi155 – 1551MagnesiumBy similarity
Metal bindingi157 – 1571MagnesiumBy similarity
Metal bindingi315 – 3151MagnesiumBy similarity
Metal bindingi320 – 3201Zinc 1By similarity
Metal bindingi324 – 3241Zinc 1By similarity
Metal bindingi361 – 3611Zinc 2By similarity
Metal bindingi362 – 3621Zinc 2By similarity
Metal bindingi437 – 4371Zinc 1By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
AP
OrganismiGadus morhua (Atlantic cod)Curated
Taxonomic identifieri8049 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

Subcellular locationi

  • Cell membrane 1 Publication; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Alkaline phosphatasePRO_0000186166Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...)1 Publication
Disulfide bondi165 ↔ 1851 Publication
Glycosylationi214 – 2141N-linked (GlcNAc...)1 Publication
Glycosylationi413 – 4131N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP83456.
SMRiP83456. Positions 3-475.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Phylogenomic databases

HOVERGENiHBG007345.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AGFPEQEPEP KFWNDWAQKT LDKALSLQTL NKNKAQNLIL FLGDGMGVPT
60 70 80 90 100
VTAARILKGQ LRGQPGEEGQ LEMDKFPFVA LSKTYNTNAQ VADSAGTATA
110 120 130 140 150
YLCGVKANEG TVGVSAAAVR SQANTTQGNE VTSILRWAKD AGKSIGIVTT
160 170 180 190 200
TRVNHATPSA AYAHCVDRDW YSDNEMPADA VEAGCKDIAR QLFENIPDID
210 220 230 240 250
VIMGGGRKYM YPKNTTDVEY PGQPKHSGTR KDGRNLVKEW VDRNTEKKGH
260 270 280 290 300
YVWNKKDLLS LNPTKVDYLL GLFEPADLPY DLERNKETDP SLSEMVEVAI
310 320 330 340 350
KILRRNPNGF YLLVEGGRID HGHHEGKDKQ AIHEAVEMDR AIGRADLMTS
360 370 380 390 400
TSDTLTVVTA DHSHLFSFGG YTPRGNEIFG LAAFISDVDQ KPFTAILYGN
410 420 430 440 450
GPGYKLVNGA RENVSTVDYQ DNSYLAQAAV PLSSETHGGE DVAVFAKGPM
460 470
AHLLHGVHEQ NYIPHAMAYA ACIGQNR
Length:477
Mass (Da):52,198
Last modified:December 15, 2003 - v1
Checksum:i0637907C62188DF9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence uncertaintyi8 – 81P or K
Sequence uncertaintyi351 – 3511T or A

Mass spectrometryi

Molecular mass is 61000±1100 Da from positions 1 - 477. Determined by MALDI. 1 Publication

Cross-referencesi

3D structure databases

ProteinModelPortaliP83456.
SMRiP83456. Positions 3-475.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG007345.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Amino acid sequence of the cold-active alkaline phosphatase from Atlantic cod (Gadus morhua)."
    Asgeirsson B., Nielsen B.N., Hoejrup P.
    Comp. Biochem. Physiol. 136B:45-60(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY, DISULFIDE BOND, GLYCOSYLATION, 3D-STRUCTURE MODELING.
    Tissue: Small intestine1 Publication.

Entry informationi

Entry nameiPPB_GADMO
AccessioniPrimary (citable) accession number: P83456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: December 15, 2003
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.