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P83456

- PPB_GADMO

UniProt

P83456 - PPB_GADMO

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Protein

Alkaline phosphatase

Gene
N/A
Organism
Gadus morhua (Atlantic cod)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.1 Publication

Cofactori

Binds 1 magnesium ion By similarity.
Binds 2 zinc ions By similarity.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi44 – 441Magnesium By similarity
Metal bindingi44 – 441Zinc 2 By similarityBy similarity
Active sitei94 – 941Phosphoserine intermediateBy similarity
Metal bindingi155 – 1551Magnesium By similarityBy similarity
Metal bindingi157 – 1571Magnesium By similarityBy similarity
Metal bindingi315 – 3151Magnesium By similarityBy similarity
Metal bindingi320 – 3201Zinc 1 By similarityBy similarity
Metal bindingi324 – 3241Zinc 1 By similarityBy similarity
Metal bindingi361 – 3611Zinc 2 By similarityBy similarity
Metal bindingi362 – 3621Zinc 2 By similarityBy similarity
Metal bindingi437 – 4371Zinc 1 By similarityBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
AP
OrganismiGadus morhua (Atlantic cod)
Taxonomic identifieri8049 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiNeoteleosteiAcanthomorphataZeiogadariaGadariaeGadiformesGadoideiGadidaeGadus

Subcellular locationi

Cell membrane; Lipid-anchorGPI-anchor 1 Publication

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Alkaline phosphatasePRO_0000186166Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...)1 Publication
Disulfide bondi165 ↔ 1851 Publication
Glycosylationi214 – 2141N-linked (GlcNAc...)1 Publication
Glycosylationi413 – 4131N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP83456.
SMRiP83456. Positions 3-475.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG007345.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P83456-1 [UniParc]FASTAAdd to Basket

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AGFPEQEPEP KFWNDWAQKT LDKALSLQTL NKNKAQNLIL FLGDGMGVPT    50
VTAARILKGQ LRGQPGEEGQ LEMDKFPFVA LSKTYNTNAQ VADSAGTATA 100
YLCGVKANEG TVGVSAAAVR SQANTTQGNE VTSILRWAKD AGKSIGIVTT 150
TRVNHATPSA AYAHCVDRDW YSDNEMPADA VEAGCKDIAR QLFENIPDID 200
VIMGGGRKYM YPKNTTDVEY PGQPKHSGTR KDGRNLVKEW VDRNTEKKGH 250
YVWNKKDLLS LNPTKVDYLL GLFEPADLPY DLERNKETDP SLSEMVEVAI 300
KILRRNPNGF YLLVEGGRID HGHHEGKDKQ AIHEAVEMDR AIGRADLMTS 350
TSDTLTVVTA DHSHLFSFGG YTPRGNEIFG LAAFISDVDQ KPFTAILYGN 400
GPGYKLVNGA RENVSTVDYQ DNSYLAQAAV PLSSETHGGE DVAVFAKGPM 450
AHLLHGVHEQ NYIPHAMAYA ACIGQNR 477
Length:477
Mass (Da):52,198
Last modified:December 15, 2003 - v1
Checksum:i0637907C62188DF9
GO

Mass spectrometryi

Molecular mass is 61000±1100 Da from positions 1 - 477. Determined by MALDI. 1 Publication

Sequence uncertainty

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence uncertaintyi8 – 81P or K1 Publication
Sequence uncertaintyi351 – 3511T or A1 Publication

Cross-referencesi

3D structure databases

ProteinModelPortali P83456.
SMRi P83456. Positions 3-475.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG007345.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Amino acid sequence of the cold-active alkaline phosphatase from Atlantic cod (Gadus morhua)."
    Asgeirsson B., Nielsen B.N., Hoejrup P.
    Comp. Biochem. Physiol. 136B:45-60(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY, DISULFIDE BOND, GLYCOSYLATION, 3D-STRUCTURE MODELING.
    Tissue: Small intestine.

Entry informationi

Entry nameiPPB_GADMO
AccessioniPrimary (citable) accession number: P83456
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: December 15, 2003
Last modified: June 11, 2014
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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