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P83456 (PPB_GADMO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Alkaline phosphatase
Short name=AP
EC=3.1.3.1
OrganismGadus morhua (Atlantic cod)
Taxonomic identifier8049 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Euteleostei › Neoteleostei › Acanthomorpha › Paracanthopterygii › Gadiformes › Gadidae › Gadus

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate. Ref.1

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer. Ref.1

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor Ref.1.

Sequence similarities

Belongs to the alkaline phosphatase family.

Biophysicochemical properties

Temperature dependence:

Thermolabile.

Mass spectrometry

Molecular mass is 61000±1100 Da from positions 1 - 477. Determined by MALDI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Alkaline phosphatase
PRO_0000186166

Sites

Active site941Phosphoserine intermediate UniProtKB P00634
Metal binding441Magnesium By similarity
Metal binding441Zinc 2 By similarity UniProtKB P00634
Metal binding1551Magnesium By similarity UniProtKB P00634
Metal binding1571Magnesium By similarity UniProtKB P00634
Metal binding3151Magnesium By similarity UniProtKB P00634
Metal binding3201Zinc 1 By similarity UniProtKB P00634
Metal binding3241Zinc 1 By similarity UniProtKB P00634
Metal binding3611Zinc 2 By similarity UniProtKB P00634
Metal binding3621Zinc 2 By similarity UniProtKB P00634
Metal binding4371Zinc 1 By similarity UniProtKB P00634

Amino acid modifications

Glycosylation1241N-linked (GlcNAc...) Ref.1
Glycosylation2141N-linked (GlcNAc...) Ref.1
Glycosylation4131N-linked (GlcNAc...) Ref.1
Disulfide bond165 ↔ 185 Ref.1

Experimental info

Sequence uncertainty81P or K Ref.1
Sequence uncertainty3511T or A Ref.1

Sequences

Sequence LengthMass (Da)Tools
P83456 [UniParc].

Last modified December 15, 2003. Version 1.
Checksum: 0637907C62188DF9

FASTA47752,198
        10         20         30         40         50         60 
AGFPEQEPEP KFWNDWAQKT LDKALSLQTL NKNKAQNLIL FLGDGMGVPT VTAARILKGQ 

        70         80         90        100        110        120 
LRGQPGEEGQ LEMDKFPFVA LSKTYNTNAQ VADSAGTATA YLCGVKANEG TVGVSAAAVR 

       130        140        150        160        170        180 
SQANTTQGNE VTSILRWAKD AGKSIGIVTT TRVNHATPSA AYAHCVDRDW YSDNEMPADA 

       190        200        210        220        230        240 
VEAGCKDIAR QLFENIPDID VIMGGGRKYM YPKNTTDVEY PGQPKHSGTR KDGRNLVKEW 

       250        260        270        280        290        300 
VDRNTEKKGH YVWNKKDLLS LNPTKVDYLL GLFEPADLPY DLERNKETDP SLSEMVEVAI 

       310        320        330        340        350        360 
KILRRNPNGF YLLVEGGRID HGHHEGKDKQ AIHEAVEMDR AIGRADLMTS TSDTLTVVTA 

       370        380        390        400        410        420 
DHSHLFSFGG YTPRGNEIFG LAAFISDVDQ KPFTAILYGN GPGYKLVNGA RENVSTVDYQ 

       430        440        450        460        470 
DNSYLAQAAV PLSSETHGGE DVAVFAKGPM AHLLHGVHEQ NYIPHAMAYA ACIGQNR

« Hide

References

[1]"Amino acid sequence of the cold-active alkaline phosphatase from Atlantic cod (Gadus morhua)."
Asgeirsson B., Nielsen B.N., Hoejrup P.
Comp. Biochem. Physiol. 136B:45-60(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION, MASS SPECTROMETRY, DISULFIDE BOND, GLYCOSYLATION, 3D-STRUCTURE MODELING.
Tissue: Small intestine.

Cross-references

3D structure databases

ProteinModelPortalP83456.
SMRP83456. Positions 3-475.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

HOVERGENHBG007345.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. Alkaline_phosphatase_core. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePPB_GADMO
AccessionPrimary (citable) accession number: P83456
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: December 15, 2003
Last modified: April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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