Reviewed, UniProtKB/Swiss-Prot P83451 (ASPG_ASOTA)

Names and origin

Protein names	Recommended name:     N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase     EC=3.5.1.26 Alternative name(s):     Glycosylasparaginase     Aspartylglucosaminidase       Short name=AGA     N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase Cleaved into the following 2 chains:     1- Recommended name:             Glycosylasparaginase alpha chain                 Short name=AGA subunit alpha         Alternative name(s):             p18     2- Recommended name:             Glycosylasparaginase beta chain                 Short name=AGA subunit beta         Alternative name(s):             p30
Organism	Asobara tabida (Parasitic wasp)
Taxonomic identifier	58720 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Hymenoptera › Apocrita › Ichneumonoidea › Braconidae › Alysiinae › Asobara

Protein attributes

Sequence length	40 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins By similarity. UniProtKB P20933
Catalytic activity	N(4)-(beta-N-acetyl-D-glucosaminyl)-L-asparagine + H2O = N-acetyl-beta-D-glucosaminylamine + L-aspartate. UniProtKB P20933
Subunit structure	Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers. Ref.1
Subcellular location	Secreted. Ref.1
Tissue specificity	Expressed by the venom gland. Ref.1
Post-translational modification	May be N-glycosylated. Ref.1
Sequence similarities	Belongs to the Ntn-hydrolase family.

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N4-(beta-N-acetylglucosaminyl)-L-asparaginase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›20	›20	Glycosylasparaginase alpha chain Ref.1		PRO_0000002341
	Chain	21 – ›40	›20	Glycosylasparaginase beta chain Ref.1		PRO_0000002342
Sites
	Active site	21	1	Nucleophile By similarity UniProtKB P20933
Experimental info
	Non-adjacent residues	20 – 21	2
	Non-terminal residue	40	1

Sequences

	Sequence		Length	Mass (Da)
	P83451-1 [UniParc]. Last modified October 25, 2004. Version 1. Checksum: 24CDC49700732BAD	FASTA	40	4,138
	10 20 30 40 RIIEPIAGGP FIVMTWNYPG TIGIIAVDAN GHIAVGTSTN

References

[1]

"Identification of an aspartylglucosaminidase-like protein in the venom of the parasitic wasp Asobara tabida (Hymenoptera: Braconidae)." Moreau S.J.M., Cherqui A., Doury G., Dubois F., Fourdrain Y., Sabatier L., Bulet P., Saarela J., Prevost G., Giordanengo P. Insect Biochem. Mol. Biol. 34:485-492(2004) [PubMed: 15110870] [Abstract] Cited for: PROTEIN SEQUENCE, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GLYCOSYLATION. Tissue: Venom.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.5.1.26. 294119.
Family and domain databases
InterPro	IPR000246. Peptidase_T2. [Graphical view]
Pfam	PF01112. Asparaginase_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPG_ASOTA

Accession

Primary (citable) accession number: P83451
Secondary accession number(s): P83452

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	October 25, 2004
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)