Reviewed, UniProtKB/Swiss-Prot P83449 (CLRB_PSECO)

Names and origin

Protein names	Recommended name:     Chlorate reductase subunit beta     EC=1.97.1.1
Organism	Pseudomonas chloritidismutans
Taxonomic identifier	203192 [NCBI]
Taxonomic lineage	Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	24 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Reduces chlorate and bromate. Ref.1
Catalytic activity	AH2 + chlorate = A + H2O + chlorite. Ref.1
Cofactor	Iron. Ref.1 Molybdenum (molybdopterin). Ref.1
Subunit structure	Heterotrimer of alpha, beta and gamma subunits. Ref.1
Subcellular location	Cytoplasm. Ref.1
biophysicochemical properties	Kinetic parameters: KM=159 µM for chlorate Vmax=51 µmol/min/mg enzyme pH dependence: Optimum pH is 7.5.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Molybdenum
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chlorate reductase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›24	›24	Chlorate reductase subunit beta		PRO_0000089871
Experimental info
	Non-terminal residue	24	1

Sequences

	Sequence		Length	Mass (Da)
	P83449-1 [UniParc]. Last modified October 1, 2003. Version 1. Checksum: B149C1D1CC24578A	FASTA	24	2,540
	10 20 TVKXQLSMVL DLNKEIGGQT XTAA

References

[1]

"Characterization of the chlorate reductase from Pseudomonas chloritidismutans." Wolterink A.F.W.M., Schiltz E., Hagedoorn P.-L., Hagen W.R., Kengen S.W.M., Stams A.J.M. J. Bacteriol. 185:3210-3213(2003) [PubMed: 12730181] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.97.1.1. 288788.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

CLRB_PSECO

Accession

Primary (citable) accession number: P83449

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 30, 2005
Last sequence update:	October 1, 2003
Last modified:	June 16, 2009
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)