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Reviewed, UniProtKB/Swiss-Prot P83394 (ITR3_CYCPE)

Last modified June 16, 2009. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Trypsin inhibitor 3
Alternative name(s):
    Trypsin inhibitor III
    CyPTI-III
Cleaved into the following chain:
    1- Recommended name:
            Trypsin inhibitor 1
        Alternative name(s):
            Trypsin inhibitor I
            CyPTI-I
OrganismCyclanthera pedata (Achocha) (Caihua)
Taxonomic identifier198836 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids ICucurbitalesCucurbitaceaeCyclanthera

Protein attributes

Sequence length29 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Strongly inhibits trypsin, weakly inhibits chymotrypsin.

Subcellular location

Secreted.

Domain

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin By similarity.

Sequence similarities

Belongs to the protease inhibitor I7 (squash-type serine protease inhibitor) family. [View classification]

Ontologies

Keywords
   Cellular componentSecreted
   DomainKnottin
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMDisulfide bond
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 2929Trypsin inhibitor 3
PRO_0000033204
Peptide2 – 2928Trypsin inhibitor 1
PRO_0000033205

Sites

Site5 – 62Reactive bond

Amino acid modifications

Disulfide bond3 ↔ 20 By similarity UniProtKB P01074
Disulfide bond10 ↔ 22 By similarity UniProtKB P01074
Disulfide bond16 ↔ 28 By similarity UniProtKB P01074

Sequences

Sequence LengthMass (Da)Tools
P83394-1 [UniParc].

Last modified October 1, 2002. Version 1.
Checksum: D5FEFF8A5FEDCC2F

FASTA293,195
        10         20 
RICPRILMEC KADSDCLAQC ICEESGFCG 

« Hide

References

[1]"Non-conventional affinity chromatography of serine proteinases and their inhibitors."
Polanowski A., Wilimowska-Pelc A., Kowalska J., Grybel J., Zelazko M., Wilusz T.
Acta Biochim. Pol. 50:765-773(2003) [PubMed: 14515156] [Abstract]
Cited for: PROTEIN SEQUENCE, REACTIVE SITE.
Tissue: Seed.
[2]"Isolation and primary structures of seven serine proteinase inhibitors from Cyclanthera pedata seeds."
Kowalska J., Zablocka A., Wilusz T.
Biochim. Biophys. Acta 1760:1054-1063(2006) [PubMed: 16635550] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, REACTIVE SITE.
Tissue: Seed.

Cross-references

3D structure databases

HSSPHSSP built from PDB template 1CTI based on UniProtKB P01074.
ModBaseSearch...

Family and domain databases

InterProIPR000737. Prot_inh_squash.
[Graphical view]
PfamPF00299. Squash. 1 hit.
[Graphical view]
PRINTSPR00293. SQUASHINHBTR.
ProDomPD003401. Prot_inh_squash. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00286. PTI. 1 hit.
[Graphical view]
PROSITEPS00286. SQUASH_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameITR3_CYCPE
AccessionPrimary (citable) accession number: P83394
Secondary accession number(s): P83392
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 2002
Last sequence update: October 1, 2002
Last modified: June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents