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P83373

- MDHM_FRAAN

UniProt

P83373 - MDHM_FRAAN

Protein

Malate dehydrogenase, mitochondrial

Gene

MMDHI

Organism
Fragaria ananassa (Strawberry)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (01 Oct 2002)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.CuratedPROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei59 – 591NADBy similarity
    Binding sitei106 – 1061SubstratePROSITE-ProRule annotation
    Binding sitei112 – 1121SubstratePROSITE-ProRule annotation
    Binding sitei119 – 1191NADBy similarity
    Binding sitei144 – 1441SubstratePROSITE-ProRule annotation
    Binding sitei178 – 1781SubstratePROSITE-ProRule annotation
    Active sitei202 – 2021Proton acceptorBy similarity
    Binding sitei253 – 2531NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi33 – 397NADBy similarity
    Nucleotide bindingi142 – 1443NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, mitochondrial (EC:1.1.1.37)
    Gene namesi
    Name:MMDHI
    OrganismiFragaria ananassa (Strawberry)Curated
    Taxonomic identifieri3747 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsRosalesRosaceaeRosoideaePotentilleaeFragariinaeFragaria

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 1919Mitochondrion1 PublicationAdd
    BLAST
    Chaini20 – 339320Malate dehydrogenase, mitochondrialPRO_0000018627Add
    BLAST

    Proteomic databases

    PRIDEiP83373.

    Interactioni

    Subunit structurei

    Homodimer.By similarityCurated

    Structurei

    3D structure databases

    ProteinModelPortaliP83373.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P83373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRPSMSLIRS VSRVARRGYS SESVPQRKVA VLGAAGGIGQ PLALLMKLNP    50
    LVSQLSLYDI AGTPGVAADV SHINTRSEVK GYAGEEQLGE ALEGCDVVII 100
    PAGVPRKPGM TRDDLFNINA GIVRSLTAAI AKYCPHAIIN MISNPVNSTV 150
    PIASEVLKKA GVYDEKKLFG VTTLDVVRAK TFYAGKAGVP VAEVNVPVVG 200
    GHAGITILPL FSQATPKANL SDDYIKALTK RTQDGGTEVV EAKAGKGSAT 250
    LSMAYAGALF ADACLXGLNG VPDVVECSYV QSSITELPFF ASKVRLGKNG 300
    VEEVLDLGPL SDFEKEGLKQ LKPELKSSIE KGIKFANQS 339
    Length:339
    Mass (Da):35,622
    Last modified:October 1, 2002 - v1
    Checksum:i22D4EF65E225A00C
    GO

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P83373.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P83373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification, cloning and expression analysis of strawberry (Fragaria x ananassa) mitochondrial citrate synthase and mitochondrial malate dehydrogenase."
      Iannetta P.P.M., Escobar N.M., Ross H.A., Souleyre E.J., Hancock R.D., Witte C.P., Davies H.V.
      Physiol. Plantarum 121:15-26(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 20-37.
      Strain: cv. Elsanta.
      Tissue: Fruit.

    Entry informationi

    Entry nameiMDHM_FRAAN
    AccessioniPrimary (citable) accession number: P83373
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: October 1, 2002
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3