ID   CISY_FRAAN              Reviewed;         469 AA.
AC   P83372;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2002, sequence version 1.
DT   13-SEP-2023, entry version 83.
DE   RecName: Full=Citrate synthase, mitochondrial;
DE            EC=2.3.3.16;
DE   Flags: Precursor;
GN   Name=MCSI;
OS   Fragaria ananassa (Strawberry) (Fragaria chiloensis x Fragaria virginiana).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Rosoideae; Potentilleae; Fragariinae;
OC   Fragaria.
OX   NCBI_TaxID=3747 {ECO:0000305};
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE, AND PROTEIN SEQUENCE OF 29-46.
RC   STRAIN=cv. Elsanta; TISSUE=Fruit;
RX   PubMed=15086813; DOI=10.1111/j.0031-9317.2004.00302.x;
RA   Iannetta P.P.M., Escobar N.M., Ross H.A., Souleyre E.J., Hancock R.D.,
RA   Witte C.P., Davies H.V.;
RT   "Identification, cloning and expression analysis of strawberry (Fragaria x
RT   ananassa) mitochondrial citrate synthase and mitochondrial malate
RT   dehydrogenase.";
RL   Physiol. Plantarum 121:15-26(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.16; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P00889}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism. {ECO:0000250|UniProtKB:P00889}.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P83372; -.
DR   SMR; P83372; -.
DR   BRENDA; 2.3.3.16; 2320.
DR   UniPathway; UPA00223; UER00717.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; IEA:InterPro.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   NCBIfam; TIGR01793; cit_synth_euk; 1.
DR   PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR11739:SF8; CITRATE SYNTHASE, MITOCHONDRIAL; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Mitochondrion; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..28
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:15086813"
FT   CHAIN           29..469
FT                   /note="Citrate synthase, mitochondrial"
FT                   /id="PRO_0000005488"
FT   ACT_SITE        304
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        350
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        405
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   469 AA;  52283 MW;  CD80AAED06246F65 CRC64;
     MAFFRTVTKL RSRLGQPPSL RDSVRCLQTQ ASSDLDLHSQ LKELIPEQQE RLKKLKKEHG
     KVQLGTITVD MVIGGMRGMT GLLWETSLLD PDEGIRFRGL SIPECQKVLP GATPGGEPLP
     EGLLWLLLTG KVPSRSKNMH YPVNNGVVPK FQIMCSRPLM LCLLEHIPMT QFTTGVMALQ
     VQSEFQKAYD KGIPKSRYWE PTYEDSLSLI AQLPVVASYV YRRIYKGGRM IPVDDSLDYG
     GNFSHLLGFD DHKMQELMRL YVTIHSDHEG GNVSAHTGHL VASALSDPFL SFAAALNGLA
     GPLHGLANQE VLLWIKSVVD ECGENITKDQ LKDYVWKTLN SGKVVPGFGH GVLRKTDPRY
     TCQREFALKH LPDDPLFRLV SKLYDVVPPI LTELGKVKNP WPNVDAHSGV LLNHFGLTEA
     RYFTVLFGVS RSIGIGSQLI WDRALGLPLE RPKSVTMESL ESFCKKAAS
//