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Protein

Venom prothrombin activator hopsarin-D

Gene
N/A
Organism
Hoplocephalus stephensii (Stephens' banded snake)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Snake prothrombin activator that attacks the hemostatic system of prey. This protein is functionally similar to blood coagulation factor Xa. The procoagulant activity of hopsarin-D is approximately 10-fold lower than that of trocarin-D and FXa.1 Publication

Miscellaneous

Is classified in the group D of snake venom prothrombin activators, since it requires the mammalian factor Va for maximal activity for the cleavage of prothrombin.
In contrast to blood coagulation factors that circulate as inactive zymogen in plasma, venom prothrombin activators are always found in the active form in the venom.

Caution

Lacks the Cys residue in position 216 that is replaced by a Ser residue, resulting of a loss a disulfide bond. This may contribute to the lower procoagulant activity.Curated

Catalytic activityi

Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei251Charge relay systemBy similarity1
Active sitei308Charge relay systemBy similarity1
Active sitei405Charge relay systemBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionBlood coagulation cascade activating toxin, Hemostasis impairing toxin, Hydrolase, Protease, Prothrombin activator, Serine protease, Toxin
LigandCalcium

Protein family/group databases

MEROPSiS01.426

Names & Taxonomyi

Protein namesi
Recommended name:
Venom prothrombin activator hopsarin-D (EC:3.4.21.6)
Short name:
vPA
Alternative name(s):
Venom coagulation factor Xa-like protease
Cleaved into the following 2 chains:
OrganismiHoplocephalus stephensii (Stephens' banded snake)Curated
Taxonomic identifieri196418 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeNotechinaeHoplocephalus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
PropeptideiPRO_000040972221 – 40By similarityAdd BLAST20
ChainiPRO_000002781641 – 181Hopsarin-D light chainAdd BLAST141
PropeptideiPRO_5000095361182 – 209Activation peptide1 PublicationAdd BLAST28
ChainiPRO_0000027817210 – 455Hopsarin-D heavy chainAdd BLAST246

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei464-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei474-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei544-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei564-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Disulfide bondi57 ↔ 62By similarity
Modified residuei594-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei604-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei654-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei664-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei694-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei724-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Modified residuei754-carboxyglutamatePROSITE-ProRule annotation1 Publication1
Disulfide bondi90 ↔ 101By similarity
Glycosylationi92O-linked (Hex...) serine1 Publication1
Disulfide bondi95 ↔ 110By similarity
Disulfide bondi112 ↔ 121By similarity
Disulfide bondi129 ↔ 140By similarity
Disulfide bondi136 ↔ 149By similarity
Disulfide bondi151 ↔ 164By similarity
Disulfide bondi172 ↔ 328Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi236 ↔ 252By similarity
Glycosylationi254N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi376 ↔ 390By similarity
Disulfide bondi401 ↔ 429By similarity

Post-translational modificationi

The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei103Not modified1

Keywords - PTMi

Disulfide bond, Gamma-carboxyglutamic acid, Glycoprotein

PTM databases

iPTMnetiP83370

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Interactioni

Subunit structurei

Heterodimer of a light chain and a heavy chain; disulfide-linked.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP83370
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini41 – 86GlaPROSITE-ProRule annotationAdd BLAST46
Domaini86 – 121EGF-like 1; calcium-bindingPROSITE-ProRule annotationAdd BLAST36
Domaini129 – 164EGF-like 2PROSITE-ProRule annotationAdd BLAST36
Domaini210 – 453Peptidase S1PROSITE-ProRule annotationAdd BLAST244

Sequence similaritiesi

Belongs to the peptidase S1 family. Snake venom subfamily.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal

Phylogenomic databases

HOVERGENiHBG013304

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
Gene3Di4.10.740.10, 1 hit
InterProiView protein in InterPro
IPR017857 Coagulation_fac-like_Gla_dom
IPR001881 EGF-like_Ca-bd_dom
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
IPR000152 EGF-type_Asp/Asn_hydroxyl_site
IPR018097 EGF_Ca-bd_CS
IPR035972 GLA-like_dom_SF
IPR000294 GLA_domain
IPR012224 Pept_S1A_FX
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00008 EGF, 1 hit
PF00594 Gla, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001143 Factor_X, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
PR00001 GLABLOOD
SMARTiView protein in SMART
SM00181 EGF, 2 hits
SM00179 EGF_CA, 1 hit
SM00069 GLA, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 2 hits
SSF57630 SSF57630, 1 hit
PROSITEiView protein in PROSITE
PS00010 ASX_HYDROXYL, 1 hit
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit
PS01187 EGF_CA, 1 hit
PS00011 GLA_1, 1 hit
PS50998 GLA_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQLLLCLI LTFLWSVPEA ESNVFLKSKV ANRFLQRTKR SNSLFEEIRP
60 70 80 90 100
GNIERECIEE KCSKEEAREV FEDNEKTETF WNVYVDGDQC SSNPCHYHGT
110 120 130 140 150
CKDGIGSYTC TCLPNYEGKN CEKVLFKSCR AFNGNCWHFC KRVQSETQCS
160 170 180 190 200
CAESYRLGVD GHSCVAEGDF SCGRNIKARN KREASLPDFV QSQKATLLKK
210 220 230 240 250
SDNPSPDIRI VNGMDSKLGE CPWQAVLINE KGEVFCGGTI LSPIHVLTAA
260 270 280 290 300
HCINQTKSVS VIVGEIDISR KETRRLLSVD KIYVHTKFVP PNYYYGHQNF
310 320 330 340 350
DRVAYDYDIA IIRMKTPIQF SENVVPACLP TADFANEVLM KQDSGIVSGF
360 370 380 390 400
GRIRFKEPTS NTLKVITVPY VDRHTCMLSS DFRITQNMFC AGYDTLPQDA
410 420 430 440 450
CEGDSGGPHI TAYGDTHFIT GIVSWGEGCA RKGKYGVYTK VSRFIPWIKK

IMSLK
Length:455
Mass (Da):51,248
Last modified:June 28, 2011 - v2
Checksum:i39B5F4442B995E4F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti78E → T AA sequence (PubMed:12403650).Curated1
Sequence conflicti98H → R AA sequence (PubMed:12403650).Curated1
Sequence conflicti260 – 270Missing AA sequence (PubMed:12403650).CuratedAdd BLAST11
Sequence conflicti402E → Q AA sequence (PubMed:12403650).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY940208 mRNA Translation: AAX37264.1

Similar proteinsi

Entry informationi

Entry nameiFAXD_HOPST
AccessioniPrimary (citable) accession number: P83370
Secondary accession number(s): Q58L92
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 11, 2003
Last sequence update: June 28, 2011
Last modified: March 28, 2018
This is version 106 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

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