P83325 (GSTP2_BUFBU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase P 2 EC=2.5.1.18 Alternative name(s): BBGSTP2-2 GST class-pi
Organism	Bufo bufo (European toad)
Taxonomic identifier	8384 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Neobatrachia › Hyloidea › Bufonidae › Bufo

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Ref.1
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.1
Subunit structure	Homodimer. Ref.1
Tissue specificity	Liver, kidney, muscle, skin, lung and ovary. Ref.1
Sequence similarities	Belongs to the GST superfamily. Pi family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Glutathione S-transferase P 2		PRO_0000185909
Regions
	Domain	1 – 82	82	GST N-terminal
	Domain	83 – 204	122	GST C-terminal
	Region	53 – 54	2	Glutathione binding By similarity
	Region	66 – 67	2	Glutathione binding By similarity
Sites
	Binding site	7	1	Glutathione By similarity
	Binding site	13	1	Glutathione By similarity
	Binding site	38	1	Glutathione By similarity
	Binding site	46	1	Glutathione By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83325 [UniParc]. Last modified May 27, 2002. Version 1. Checksum: 3D233F9AE53F3F8C	FASTA	210	24,179
	10 20 30 40 50 60 SGYTLTYFPL RGRAEAMRLL LGDQGVSWTD DEVQMQDWAA GIRDLKKNAV FGQIPRFQEG 70 80 90 100 110 120 DFVLYQSQTI LRLLARYGLS GSNEREIAIN EMMNDGVEDL RLKYYKFIFW DNEANKEKFL 130 140 150 160 170 180 EELATQLGYF ERILTNNAGK TFVLVGDKIS YADYNLLDTL FCVLDLSPTC LSGFPLLSDY 190 200 210 VERLGKRPKL QQYLKSEGRK RRPINGNGKQ

References

[1]

"Amino acid sequence of the major form of toad liver glutathione transferase." Bucciarelli T., Sacchetta P., Amicarelli F., Petruzzelli R., Melino S., Rotilio D., Celli N., Di Ilio C. Int. J. Biochem. Cell Biol. 34:1286-1290(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, TISSUE SPECIFICITY. Tissue: Liver.

Cross-references

3D structure databases
ProteinModelPortal	P83325.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG108324.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR003082. GST_pi. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
PRINTS	PR01268. GSTRNSFRASEP.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTP2_BUFBU

Accession

Primary (citable) accession number: P83325

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 27, 2002
Last sequence update:	May 27, 2002
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program