Reviewed, UniProtKB/Swiss-Prot P83324 (RIP_FLAVE)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein velutin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Flammulina velutipes
Taxonomic identifier	38945 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Tricholomataceae › Flammulina

Protein attributes

Sequence length	25 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits protein synthesis but does not possess ribonuclease activity. Also inhibits HIV-1 reverse transcriptase, beta-glucosidase and beta-glucuronidase. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family.

Ontologies

Keywords
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of translation Ref.1 Inferred from direct assay. Source: UniProtKB pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›25	›25	Ribosome-inactivating protein velutin		PRO_0000221420
Experimental info
	Non-terminal residue	25	1

Sequences

	Sequence		Length	Mass (Da)
	P83324-1 [UniParc]. Last modified June 1, 2002. Version 1. Checksum: EB73FCF9AA0993DB	FASTA	25	2,898
	10 20 XHPDLFXXRP DNTASPKFED PRLNP

References

[1]	"Isolation and characterization of velutin, a novel low-molecular-weight ribosome-inactivating protein from winter mushroom (Flammulina velutipes) fruiting bodies." Wang H., Ng T.B. Life Sci. 68:2151-2158(2001) [PubMed: 11324720] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 74554.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

RIP_FLAVE

Accession

Primary (citable) accession number: P83324

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	June 1, 2002
Last modified:	January 20, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)