Reviewed, UniProtKB/Swiss-Prot P83323 (RIP_SIRGR)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein momorgrosvin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Siraitia grosvenorii (Monk's fruit) (Luo han guo)
Taxonomic identifier	190515 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Siraitia

Protein attributes

Sequence length	18 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits protein synthesis in the rabbit reticulocyte lysate system. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Post-translational modification	Glycosylated. Ref.1
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Ref.1 Inferred from direct assay. Source: UniProtKB pathogenesis Ref.1 Traceable author statement. Source: UniProtKB
Molecular function	rRNA N-glycosylase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›18	›18	Ribosome-inactivating protein momorgrosvin		PRO_0000221407
Experimental info
	Non-terminal residue	18	1

Sequences

	Sequence		Length	Mass (Da)
	P83323-1 [UniParc]. Last modified May 2, 2002. Version 1. Checksum: 476E15CE683E2446	FASTA	18	2,032
	10 DVTFSMLGAN GATYYQFF

References

[1]	"Isolation and characterization of a new ribosome inactivating protein, momorgrosvin, from seeds of the monk's fruit Momordica grosvenorii." Tsang K.Y., Ng T.B. Life Sci. 68:773-784(2001) [PubMed: 11205869] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, GLYCOSYLATION. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 293262.
Family and domain databases
InterPro	IPR017988. Ribosome_inactivat_prot_CS. [Graphical view]
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP_SIRGR

Accession

Primary (citable) accession number: P83323

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 2, 2002
Last sequence update:	May 2, 2002
Last modified:	March 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)