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Protein

Mu-theraphotoxin-Hs2a

Gene
N/A
Organism
Haplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This lethal neurotoxin acts selectively on tetrodotoxin-sensitive (TTX-S) voltage-gated sodium channels (Nav), with an IC50 of 30 nM in rat DRG neurons. Preferentially inhibits neuronal voltage-gated sodium channel subtype hNav1.7/SCN9A (IC50 is 26 nM), rNav1.2/SCN2A (IC50 is 150 nM), and rNav1.3/SCN3A (IC50 is 338 nM), compared with muscle subtypes rNav1.4/SCN4A and hNav1.5/SCN5A (IC50 is > 10 µM). Inhibits activation of sodium channel by trapping the voltage sensor of domain II of the site 4 in the inward, closed configuration.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin

Protein family/group databases

TCDBi8.B.3.1.3. the huwentoxin-1 (huwentoxin-1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Mu-theraphotoxin-Hs2a
Short name:
Mu-TRTX-Hs2a
Alternative name(s):
Huwentoxin-4
Huwentoxin-IV
Short name:
HwTx-IV
Huwentoxin-IVa
Short name:
HWTX-IVa
Huwentoxin-IVb
Short name:
HWTX-IVb
Huwentoxin-IVc
Short name:
HWTX-IVc
OrganismiHaplopelma schmidti (Chinese bird spider) (Ornithoctonus huwenum)
Taxonomic identifieri29017 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeMygalomorphaeTheraphosidaeHaplopelma

Organism-specific databases

ArachnoServeriAS000332. mu-theraphotoxin-Hs2a.

Subcellular locationi

  • Secreted 1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000003556025 – 521 PublicationAdd BLAST28
ChainiPRO_000003556153 – 87Mu-theraphotoxin-Hs2a1 PublicationAdd BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi54 ↔ 691 Publication
Disulfide bondi61 ↔ 761 Publication
Disulfide bondi68 ↔ 831 Publication
Modified residuei87Isoleucine amide1 Publication1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

Secondary structure

189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi57 – 60Combined sources4
Beta strandi63 – 65Combined sources3
Turni70 – 73Combined sources4
Beta strandi74 – 77Combined sources4
Turni78 – 81Combined sources4
Beta strandi82 – 85Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1MB6NMR-A53-87[»]
2M4XNMR-A53-87[»]
2M4ZNMR-A53-87[»]
2M50NMR-A53-87[»]
5TLRNMR-A54-87[»]
ProteinModelPortaliP83303.
SMRiP83303.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83303.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Sequence similaritiesi

Belongs to the huwentoxin-1 family. Htx-4 subfamily.Curated

Keywords - Domaini

Knottin, Signal

Family and domain databases

InterProiView protein in InterPro
IPR011696. Huwentoxin-1.
IPR013140. Huwentoxin_CS1.
PfamiView protein in Pfam
PF07740. Toxin_12. 1 hit.
PROSITEiView protein in PROSITE
PS60021. HWTX_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83303-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNMKASMFL ALAGLVLLFV VCYASESEEK EFSNELLSSV LAVDDNSKGE
60 70 80
ERECLEIFKA CNPSNDQCCK SSKLVCSRKT RWCKYQIGK
Length:89
Mass (Da):9,997
Last modified:November 23, 2004 - v2
Checksum:i39CCC6FC1ADCBC75
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17L → Q in ABY77746 (PubMed:18482741).Curated1
Sequence conflicti85Y → C in ABY77745 (PubMed:18482741).Curated1

Mass spectrometryi

Molecular mass is 4104.7 Da from positions 53 - 87. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY263707 mRNA. Translation: AAP33074.1.
EU195291 mRNA. Translation: ABY77744.1.
EU195292 mRNA. Translation: ABY77745.1.
EU195293 mRNA. Translation: ABY77746.1.

Similar proteinsi

Entry informationi

Entry nameiTXH4_HAPSC
AccessioniPrimary (citable) accession number: P83303
Secondary accession number(s): B3FIU7
, B3FIU8, B3FIU9, Q86C52
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: November 23, 2004
Last modified: April 12, 2017
This is version 82 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families