Reviewed, UniProtKB/Swiss-Prot P83291 (NCB5R_ARATH)

Names and origin

Protein names

Recommended name:
    NADH-cytochrome b5 reductase-like protein
      Short name=B5R
    EC=1.6.2.2

Gene names

Ordered Locus Names:	At5g20080
ORF Names:	F28I16.230

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Desaturation and elongation of fatty acids By similarity.
Catalytic activity	NADH + 2 ferricytochrome b5 = NAD+ + H+ + 2 ferrocytochrome b5.
Cofactor	FAD.
Subcellular location	Mitochondrion intermembrane space Ref.3 Ref.4.
Sequence similarities	Belongs to the flavoprotein pyridine nucleotide cytochrome reductase family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to salt stress Inferred from expression pattern. Source: TAIR
Cellular component	mitochondrial intermembrane space Ref.3 Traceable author statement. Source: UniProtKB plant-type cell wall Inferred from direct assay. Source: TAIR
Molecular function	cytochrome-b5 reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	NADH-cytochrome b5 reductase-like protein		PRO_0000167626
Regions
	Domain	76 – 184	109	FAD-binding FR-type

Sequences

	Sequence		Length	Mass (Da)
	P83291-1 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 9C25F8EFBEA60E46	FASTA	328	35,987
	10 20 30 40 50 60 MATSFFRRLA RSAPITFPVA FGSQSKSGSG AFRFSTGAIA ALSGGFSYYY LTSGNNLVYL 70 80 90 100 110 120 DQAKEETGPK TALNPDKWLE FKLQDTARVS HNTQLFRFSF DPSAELGLHV ASCLLTRAPL 130 140 150 160 170 180 GYNAEGKTKY VIRPYTPISD PEAKGYFDLL IKVYPDGKMS QHFASLKPGD VLEVKGPVEK 190 200 210 220 230 240 FKYSPNMKKH IGMIAGGSGI TPMLQVIDAI VKNPEDNTQI SLLYANVSPD DILLKQKLDV 250 260 270 280 290 300 LQANHPNLKI FYTVDNPTKN WKGGVGYISK DMALKGLPLP TDDTLILVCG PPGMMEHISG 310 320 GKAPDWSQGE VKGILKELGY TEEMVFKF

References

[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed: 11130714] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]	"Proteomic approach to identify novel mitochondrial proteins in Arabidopsis." Kruft V., Eubel H., Jaensch L., Werhahn W., Braun H.-P. Plant Physiol. 127:1694-1710(2001) [PubMed: 11743114] [Abstract] Cited for: PROTEIN SEQUENCE OF 61-74, SUBCELLULAR LOCATION. Tissue: Leaf and Stem.
[4]	"Experimental analysis of the Arabidopsis mitochondrial proteome highlights signaling and regulatory components, provides assessment of targeting prediction programs, and indicates plant-specific mitochondrial proteins." Heazlewood J.L., Tonti-Filippini J.S., Gout A.M., Day D.A., Whelan J., Millar A.H. Plant Cell 16:241-256(2004) [PubMed: 14671022] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF296836 Genomic DNA. No translation available. AY087280 mRNA. Translation: AAM64833.1.
IPI	IPI00538985.
RefSeq	NP_568391.1.
UniGene	At.19868 Rra.2627 Rsa.16450
3D structure databases
SMR	P83291. Positions 69-328.
ModBase	Search...
Proteomic databases
PRIDE	P83291.
ProMEX	P83291.
Genome annotation databases
GeneID	832130.
GenomeReviews	Gene locus AT5G20080 in contig BA000015_GR.
KEGG	ath:AT5G20080.
Organism-specific databases
GeneFarm	2029.
TAIR	At5g20080.
Phylogenomic databases
eggNOG	KOG0534.
HOGENOM	HBG591994.
InParanoid	P83291.
OMA	YANVSPD.
PhylomeDB	P83291.
Enzyme and pathway databases
BRENDA	1.6.2.2. 302.
Gene expression databases
ArrayExpress	P83291.
Genevestigator	P83291.
GermOnline	AT5G20080. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR001834. NADH-Cyt_B5_reductase. IPR008333. OxRdtase_FAD-bd_dom. IPR001433. OxRdtase_FAD/NAD_bd. IPR017938. Riboflavin_synthase-like_b-brl. [Graphical view]
Pfam	PF00970. FAD_binding_6. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00406. CYTB5RDTASE. PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NCB5R_ARATH

Accession

Primary (citable) accession number: P83291
Secondary accession number(s): Q8LBD3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	August 31, 2004
Last modified:	February 9, 2010
This is version 58 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)