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Protein

Eukaryotic translation initiation factor 2 subunit 1

Gene

EIF2S1

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.

GO - Molecular functioni

  • GTP binding Source: UniProtKB
  • ribosome binding Source: UniProtKB
  • translation initiation factor activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionInitiation factor, RNA-binding
Biological processProtein biosynthesis, Translation regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 2 subunit 1
Alternative name(s):
Eukaryotic translation initiation factor 2 subunit alpha
Short name:
eIF-2-alpha
Short name:
eIF-2A
Short name:
eIF-2alpha
Gene namesi
Name:EIF2S1
Synonyms:EIF2A
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmic granule By similarity

  • Note: The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity).By similarity

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001373851 – ›52Eukaryotic translation initiation factor 2 subunit 1Add BLAST›52

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48Phosphoserine; by HRI1 Publication1
Modified residuei51PhosphoserineBy similarity1

Post-translational modificationi

Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Phosphorylated; phosphorylation on Ser-51 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation.By similarity

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP83268.

Interactioni

Subunit structurei

Heterotrimer composed of an alpha, a beta and a gamma chain. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with ABCF1. Associates with ribosomes. Interaction with METAP2 protects EIF2S1 from inhibitory phosphorylation (By similarity).By similarity

Protein-protein interaction databases

IntActiP83268. 1 interactor.
STRINGi9986.ENSOCUP00000010342.

Structurei

3D structure databases

ProteinModelPortaliP83268.
SMRiP83268.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – ›52S1 motifPROSITE-ProRule annotationAdd BLAST›37

Sequence similaritiesi

Belongs to the eIF-2-alpha family.Curated

Phylogenomic databases

eggNOGiKOG2916. Eukaryota.
COG1093. LUCA.
HOGENOMiHOG000199476.
InParanoidiP83268.

Family and domain databases

InterProiView protein in InterPro
IPR012340. NA-bd_OB-fold.
IPR003029. S1_domain.
IPR011488. TIF_2_asu.
PANTHERiPTHR10602. PTHR10602. 1 hit.
PfamiView protein in Pfam
PF00575. S1. 1 hit.
SUPFAMiSSF50249. SSF50249. 1 hit.
PROSITEiView protein in PROSITE
PS50126. S1. 1 hit.

Sequencei

Sequence statusi: Fragment.

P83268-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PGLSCRFYQH KFPEVEDVVM VNVRSIAEMG AYVSLLEYNN IEGRILLSEL

SR
Length:52
Mass (Da):5,974
Last modified:March 1, 2002 - v1
Checksum:i52E63D8DCEA6B804
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence uncertaintyi101
Sequence conflicti10H → R AA sequence (PubMed:3592677).Curated1
Sequence conflicti16E → Q AA sequence (PubMed:3745199).Curated1
Sequence uncertaintyi441
Sequence uncertaintyi521
Non-terminal residuei521

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIF2A_RABIT
AccessioniPrimary (citable) accession number: P83268
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: March 1, 2002
Last modified: May 10, 2017
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. SIMILARITY comments
    Index of protein domains and families