P83252 (BGAL_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Beta-galactosidase EC=3.2.1.23 Alternative name(s): Acid beta-galactosidase Short name=Lactase Exo-(1-->4)-beta-D-galactanase Cleaved into the following 2 chains: Beta-galactosidase large subunit Beta-galactosidase small subunit
Organism	Hordeum vulgare (Barley)
Taxonomic identifier	4513 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	38 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in cell wall degradation. Degrades polysaccharides containing beta-(1-->4)-linked galactans, acting as an exo-(1-->4)-beta-D-galactanase By similarity. UniProtKB P48981
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. UniProtKB P48981
Subunit structure	Heterodimer of a large and a small subunit. Ref.1
Post-translational modification	The small subunit is N-glycosylated. Ref.1
Miscellaneous	There are three forms of the large subunit which have the same sequence but differ in charge. There are four forms of the small subunit which have the same sequence but differ in charge. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	beta-galactosidase activity Non-traceable author statement Ref.1. Source: UniProtKB cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›28	›28	Beta-galactosidase large subunit Ref.1		PRO_0000271912
	Chain	29 – ›38	›10	Beta-galactosidase small subunit Ref.1		PRO_0000271913
Experimental info
	Non-adjacent residues	28 – 29	2
	Non-terminal residue	38	1

Sequences

	Sequence		Length	Mass (Da)
	P83252 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: 7138F328FA3EFFDE	FASTA	38	4,126
	10 20 30 TGVTYDHRAL VIDGXXXVLV SGSIHYPRAA SSWYAVET

References

[1]	"Barley beta-galactosidase: structure, function, heterogeneity, and gene origin." Triantafillidou D., Georgatsos J.G. J. Protein Chem. 20:551-562(2001) [PubMed: 11838543] [Abstract] Cited for: PROTEIN SEQUENCE, SUBUNIT, GLYCOSYLATION. Strain: cv. Sofia. Tissue: Seed.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P83252.
Gene expression databases
Genevestigator	P83252.
Family and domain databases
InterPro	IPR013781. Glyco_hydro_subgr_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_HORVU

Accession

Primary (citable) accession number: P83252

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	January 9, 2007
Last modified:	May 31, 2011
This is version 33 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program