P83246 (GST_ASADI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase EC=2.5.1.18 Alternative name(s): GST class-sigma adGST
Organism	Asaphis dichotoma
Taxonomic identifier	184428 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Lophotrochozoa › Mollusca › Bivalvia › Heteroconchia › Veneroida › Tellinoidea › Psammobiidae › Asaphis

Protein attributes

Sequence length	15 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has a strong specific activity toward 1-chloro-2,4-dinitrobenzene and ethacrynic acid. Ref.1
Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Subunit structure	Homodimer. Ref.1
Miscellaneous	In A.dichotoma there are at least two isozymes of glutathione S-transferase.
Sequence similarities	Belongs to the GST superfamily. Sigma family. UniProtKB P20137
Biophysicochemical properties	Kinetic parameters: KM=0.68 mM for 1-chloro-2,4-dinitrobenzene KM=0.106 mM for glutathione Vmax=0.1446 mmol/min/mg enzyme toward CDNB Vmax=0.033 mmol/min/mg enzyme toward GSH pH dependence: Optimum pH is 8.5 with 1-chloro-2,4-dinitrobenzene as substrate.
Mass spectrometry	Molecular mass is 23138 Da from positions 1 - ?. Determined by MALDI. Ref.1

Ontologies

Keywords
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	glutathione metabolic process Inferred from direct assay Ref.1. Source: UniProtKB
Molecular_function	glutathione transferase activity Inferred from direct assay Ref.1. Source: UniProtKB protein homodimerization activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›15	›15	Glutathione S-transferase		PRO_0000185923
Experimental info
	Non-terminal residue	15	1

Sequences

	Sequence		Length	Mass (Da)
	P83246 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: CB3E4BF92D3CB0B9	FASTA	15	1,767
	10 PSYKLHYFDL RAAGE

References

[1]

"Purification and characterization of a novel glutathione S-transferase from Asaphis dichotoma." Yang H.-L., Nie L.-J., Zhu S.-G., Zhou X.-W. Arch. Biochem. Biophys. 403:202-208(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, CIRCULAR DICHROISM ANALYSIS. Tissue: Gut and Liver.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

GST_ASADI

Accession

Primary (citable) accession number: P83246

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 2002
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)