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P83246

- GST_ASADI

UniProt

P83246 - GST_ASADI

Protein

Glutathione S-transferase

Gene
N/A
Organism
Asaphis dichotoma
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Has a strong specific activity toward 1-chloro-2,4-dinitrobenzene and ethacrynic acid.1 Publication

    Catalytic activityi

    RX + glutathione = HX + R-S-glutathione.1 Publication

    Kineticsi

    1. KM=0.68 mM for 1-chloro-2,4-dinitrobenzene1 Publication
    2. KM=0.106 mM for glutathione1 Publication

    Vmax=0.1446 mmol/min/mg enzyme toward CDNB1 Publication

    Vmax=0.033 mmol/min/mg enzyme toward GSH1 Publication

    pH dependencei

    Optimum pH is 8.5 with 1-chloro-2,4-dinitrobenzene as substrate.1 Publication

    GO - Molecular functioni

    1. glutathione transferase activity Source: UniProtKB
    2. protein homodimerization activity Source: UniProtKB

    GO - Biological processi

    1. glutathione metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutathione S-transferase (EC:2.5.1.18)
    Alternative name(s):
    GST class-sigma
    adGST
    OrganismiAsaphis dichotomaCurated
    Taxonomic identifieri184428 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaLophotrochozoaMolluscaBivalviaHeteroconchiaVeneroidaTellinoideaPsammobiidaeAsaphis

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›15›15Glutathione S-transferasePRO_0000185923Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GST superfamily. Sigma family.By similarity

    Sequencei

    Sequence statusi: Fragment.

    P83246-1 [UniParc]FASTAAdd to Basket

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    PSYKLHYFDL RAAGE                                         15
    Length:15
    Mass (Da):1,767
    Last modified:March 1, 2002 - v1
    Checksum:iCB3E4BF92D3CB0B9
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei15 – 1511 Publication

    Mass spectrometryi

    Molecular mass is 23138 Da from positions 1 - ?. Determined by MALDI. 1 Publication

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of a novel glutathione S-transferase from Asaphis dichotoma."
      Yang H.-L., Nie L.-J., Zhu S.-G., Zhou X.-W.
      Arch. Biochem. Biophys. 403:202-208(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, SUBUNIT, MASS SPECTROMETRY, CIRCULAR DICHROISM ANALYSIS.
      Tissue: Gut and Liver.

    Entry informationi

    Entry nameiGST_ASADI
    AccessioniPrimary (citable) accession number: P83246
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 2002
    Last sequence update: March 1, 2002
    Last modified: October 1, 2014
    This is version 35 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Miscellaneous

    In A.dichotoma there are at least two isozymes of glutathione S-transferase.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3