Reviewed, UniProtKB/Swiss-Prot P83245 (RIP_PLETU)

Names and origin

Protein names	Recommended name:     Ribosome-inactivating protein pleuturegin     EC=3.2.2.22 Alternative name(s):     rRNA N-glycosidase
Organism	Pleurotus tuberregium (White-rot fungus)
Taxonomic identifier	38805 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Agaricomycotina › Homobasidiomycetes › Agaricomycetidae › Agaricales › Pleurotaceae › Pleurotus

Protein attributes

Sequence length	28 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits protein synthesis in animal cells. Does not possess ribonuclease activity. Ref.1
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family.

Ontologies

Keywords
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of translation Ref.1 Inferred from direct assay. Source: UniProtKB pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›28	›28	Ribosome-inactivating protein pleuturegin		PRO_0000221421
Experimental info
	Non-terminal residue	28	1

Sequences

	Sequence		Length	Mass (Da)
	P83245-1 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: 49FCAB47D3DBC98A	FASTA	28	2,961
	10 20 ARTQPGNIAP VGDFTLYPNA PRQGHIVA

References

[1]	"Isolation of pleuturegin, a novel ribosome-inactivating protein from fresh sclerotia of the edible mushroom Pleurotus tuber-regium." Wang H.X., Ng T.B. Biochem. Biophys. Res. Commun. 288:718-721(2001) [PubMed: 11676502] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION. Tissue: Sclerotium.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 302492.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

RIP_PLETU

Accession

Primary (citable) accession number: P83245

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
Last sequence update:	March 1, 2002
Last modified:	January 20, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)