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P83194

- G6PI_PYRFU

UniProt

P83194 - G6PI_PYRFU

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Protein

Glucose-6-phosphate isomerase

Gene
pgiA, PF0196
Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.UniRule annotation

Cofactori

Binds 1 iron ion per subunit By similarity.UniRule annotation

Enzyme regulationi

Inhibited by mannose 6-phosphate, fructose 1-phosphate and fructose 1,6-bisphosphate.UniRule annotation

pH dependencei

Optimum pH is 7.0.UniRule annotation

Temperature dependencei

Optimum temperature is 90-96 degrees Celsius. Highly thermostable.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi88 – 881Iron By similarity
Metal bindingi90 – 901Iron By similarity
Metal bindingi97 – 971Iron By similarity
Metal bindingi136 – 1361Iron By similarity

GO - Molecular functioni

  1. glucose-6-phosphate isomerase activity Source: UniProtKB-HAMAP
  2. iron ion binding Source: InterPro

GO - Biological processi

  1. gluconeogenesis Source: UniProtKB-HAMAP
  2. glycolytic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11807.
SABIO-RKP83194.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgiA
Ordered Locus Names:PF0196
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
ProteomesiUP000001013: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 189189Glucose-6-phosphate isomeraseUniRule annotationPRO_0000185357Add
BLAST

Proteomic databases

PRIDEiP83194.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0196.

Structurei

Secondary structure

1
189
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105
Turni12 – 143
Beta strandi22 – 265
Helixi27 – 304
Turni31 – 333
Helixi37 – 4610
Beta strandi50 – 578
Beta strandi66 – 738
Beta strandi97 – 1048
Beta strandi106 – 1116
Beta strandi117 – 1226
Beta strandi127 – 1304
Beta strandi135 – 1406
Beta strandi142 – 1443
Beta strandi146 – 1538
Helixi161 – 1666
Beta strandi169 – 1757
Beta strandi178 – 1836

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1QXJX-ray1.80A/B1-189[»]
1QXRX-ray1.70A/B1-189[»]
1QY4X-ray1.80A/B1-189[»]
1X7NX-ray1.89A1-189[»]
1X82X-ray1.50A1-189[»]
1X8EX-ray2.80A/B1-189[»]
2GC0X-ray2.00A/B1-187[»]
2GC1X-ray1.95A/B1-187[»]
2GC2X-ray2.10A/B1-187[»]
2GC3X-ray2.10A/B1-187[»]
3SXWX-ray1.80A1-189[»]
ProteinModelPortaliP83194.
SMRiP83194. Positions 1-189.

Miscellaneous databases

EvolutionaryTraceiP83194.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal-type GPI family.

Phylogenomic databases

eggNOGiCOG2140.
HOGENOMiHOG000243903.
KOiK06859.
OMAiYPADAGH.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01410. G6P_isomerase_arch.
InterProiIPR010551. G6P_isomerase_prok.
IPR016758. G6P_isomerase_subgr.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF06560. GPI. 1 hit.
[Graphical view]
PIRSFiPIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P83194-1 [UniParc]FASTAAdd to Basket

« Hide

MYKEPFGVKV DFETGIIEGA KKSVRRLSDM EGYFVDERAW KELVEKEDPV    50
VYEVYAVEQE EKEGDLNFAT TVLYPGKVGK EFFFTKGHFH AKLDRAEVYV 100
ALKGKGGMLL QTPEGDAKWI SMEPGTVVYV PPYWAHRTVN IGDEPFIFLA 150
IYPADAGHDY GTIAEKGFSK IVIEENGEVK VVDNPRWKK 189
Length:189
Mass (Da):21,476
Last modified:March 1, 2002 - v1
Checksum:iC5D8380C59F2A785
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti11 – 111D → N AA sequence 1 Publication
Sequence conflicti28 – 281S → P AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF381250 Genomic DNA. Translation: AAL27992.1.
AE009950 Genomic DNA. Translation: AAL80320.1.
RefSeqiNP_577925.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80320; AAL80320; PF0196.
GeneIDi1468028.
KEGGipfu:PF0196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF381250 Genomic DNA. Translation: AAL27992.1 .
AE009950 Genomic DNA. Translation: AAL80320.1 .
RefSeqi NP_577925.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1QXJ X-ray 1.80 A/B 1-189 [» ]
1QXR X-ray 1.70 A/B 1-189 [» ]
1QY4 X-ray 1.80 A/B 1-189 [» ]
1X7N X-ray 1.89 A 1-189 [» ]
1X82 X-ray 1.50 A 1-189 [» ]
1X8E X-ray 2.80 A/B 1-189 [» ]
2GC0 X-ray 2.00 A/B 1-187 [» ]
2GC1 X-ray 1.95 A/B 1-187 [» ]
2GC2 X-ray 2.10 A/B 1-187 [» ]
2GC3 X-ray 2.10 A/B 1-187 [» ]
3SXW X-ray 1.80 A 1-189 [» ]
ProteinModelPortali P83194.
SMRi P83194. Positions 1-189.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 186497.PF0196.

Proteomic databases

PRIDEi P83194.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAL80320 ; AAL80320 ; PF0196 .
GeneIDi 1468028.
KEGGi pfu:PF0196.

Phylogenomic databases

eggNOGi COG2140.
HOGENOMi HOG000243903.
KOi K06859.
OMAi YPADAGH.

Enzyme and pathway databases

UniPathwayi UPA00109 ; UER00181 .
BioCyci MetaCyc:MONOMER-11807.
SABIO-RK P83194.

Miscellaneous databases

EvolutionaryTracei P83194.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
HAMAPi MF_01410. G6P_isomerase_arch.
InterProi IPR010551. G6P_isomerase_prok.
IPR016758. G6P_isomerase_subgr.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view ]
Pfami PF06560. GPI. 1 hit.
[Graphical view ]
PIRSFi PIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMi SSF51182. SSF51182. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily."
    Verhees C.H., Huynen M.A., Ward D.E., Schiltz E., de Vos W.M., van der Oost J.
    J. Biol. Chem. 276:40926-40932(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20, CHARACTERIZATION.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Novel type of glucose-6-phosphate isomerase in the hyperthermophilic archaeon Pyrococcus furiosus."
    Hansen T., Oehlmann M., Schoenheit P.
    J. Bacteriol. 183:3428-3435(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 1-28, CHARACTERIZATION.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.

Entry informationi

Entry nameiG6PI_PYRFU
AccessioniPrimary (citable) accession number: P83194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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