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P83194 (G6PI_PYRFU) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate isomerase

Short name=GPI
EC=5.3.1.9
Alternative name(s):
Phosphoglucose isomerase
Short name=PGI
Phosphohexose isomerase
Short name=PHI
Gene names
Name:pgiA
Ordered Locus Names:PF0196
OrganismPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) [Reference proteome] [HAMAP]
Taxonomic identifier186497 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus

Protein attributes

Sequence length189 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

D-glucose 6-phosphate = D-fructose 6-phosphate. HAMAP-Rule MF_01410

Cofactor

Binds 1 iron ion per subunit By similarity. HAMAP-Rule MF_01410

Enzyme regulation

Inhibited by mannose 6-phosphate, fructose 1-phosphate and fructose 1,6-bisphosphate. HAMAP-Rule MF_01410

Pathway

Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4. HAMAP-Rule MF_01410

Subunit structure

Homodimer.

Subcellular location

Cytoplasm HAMAP-Rule MF_01410.

Sequence similarities

Belongs to the archaeal-type GPI family.

Biophysicochemical properties

pH dependence:

Optimum pH is 7.0. HAMAP-Rule MF_01410

Temperature dependence:

Optimum temperature is 90-96 degrees Celsius. Highly thermostable.

Ontologies

Keywords
   Biological processGluconeogenesis
Glycolysis
   Cellular componentCytoplasm
   LigandIron
Metal-binding
   Molecular functionIsomerase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processgluconeogenesis

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucose-6-phosphate isomerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

iron ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 189189Glucose-6-phosphate isomerase HAMAP-Rule MF_01410
PRO_0000185357

Sites

Metal binding881Iron By similarity
Metal binding901Iron By similarity
Metal binding971Iron By similarity
Metal binding1361Iron By similarity

Experimental info

Sequence conflict111D → N AA sequence Ref.1
Sequence conflict281S → P AA sequence Ref.3

Secondary structure

................................... 189
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P83194 [UniParc].

Last modified March 1, 2002. Version 1.
Checksum: C5D8380C59F2A785

FASTA18921,476
        10         20         30         40         50         60 
MYKEPFGVKV DFETGIIEGA KKSVRRLSDM EGYFVDERAW KELVEKEDPV VYEVYAVEQE 

        70         80         90        100        110        120 
EKEGDLNFAT TVLYPGKVGK EFFFTKGHFH AKLDRAEVYV ALKGKGGMLL QTPEGDAKWI 

       130        140        150        160        170        180 
SMEPGTVVYV PPYWAHRTVN IGDEPFIFLA IYPADAGHDY GTIAEKGFSK IVIEENGEVK 


VVDNPRWKK 

« Hide

References

« Hide 'large scale' references
[1]"The phosphoglucose isomerase from the hyperthermophilic archaeon Pyrococcus furiosus is a unique glycolytic enzyme that belongs to the cupin superfamily."
Verhees C.H., Huynen M.A., Ward D.E., Schiltz E., de Vos W.M., van der Oost J.
J. Biol. Chem. 276:40926-40932(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20, CHARACTERIZATION.
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[2]"Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
[3]"Novel type of glucose-6-phosphate isomerase in the hyperthermophilic archaeon Pyrococcus furiosus."
Hansen T., Oehlmann M., Schoenheit P.
J. Bacteriol. 183:3428-3435(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-28, CHARACTERIZATION.
Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF381250 Genomic DNA. Translation: AAL27992.1.
AE009950 Genomic DNA. Translation: AAL80320.1.
RefSeqNP_577925.1. NC_003413.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1QXJX-ray1.80A/B1-189[»]
1QXRX-ray1.70A/B1-189[»]
1QY4X-ray1.80A/B1-189[»]
1X7NX-ray1.89A1-189[»]
1X82X-ray1.50A1-189[»]
1X8EX-ray2.80A/B1-189[»]
2GC0X-ray2.00A/B1-187[»]
2GC1X-ray1.95A/B1-187[»]
2GC2X-ray2.10A/B1-187[»]
2GC3X-ray2.10A/B1-187[»]
3SXWX-ray1.80A1-189[»]
ProteinModelPortalP83194.
SMRP83194. Positions 1-189.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING186497.PF0196.

Proteomic databases

PRIDEP83194.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAL80320; AAL80320; PF0196.
GeneID1468028.
KEGGpfu:PF0196.

Phylogenomic databases

eggNOGCOG2140.
HOGENOMHOG000243903.
KOK06859.
OMAYPADAGH.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-11807.
SABIO-RKP83194.
UniPathwayUPA00109; UER00181.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
HAMAPMF_01410. G6P_isomerase_arch.
InterProIPR010551. G6P_isomerase_prok.
IPR016758. G6P_isomerase_subgr.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamPF06560. GPI. 1 hit.
[Graphical view]
PIRSFPIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMSSF51182. SSF51182. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP83194.

Entry information

Entry nameG6PI_PYRFU
AccessionPrimary (citable) accession number: P83194
Entry history
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: June 11, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways