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Protein

Glucose-6-phosphate isomerase

Gene

pgiA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

D-glucose 6-phosphate = D-fructose 6-phosphate.

Cofactori

Fe cationBy similarityNote: Binds 1 Fe cation per subunit.By similarity

Enzyme regulationi

Inhibited by mannose 6-phosphate, fructose 1-phosphate and fructose 1,6-bisphosphate.

pH dependencei

Optimum pH is 7.0.

Temperature dependencei

Optimum temperature is 90-96 degrees Celsius. Highly thermostable.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgiA)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi88IronBy similarity1
Metal bindingi90IronBy similarity1
Metal bindingi97IronBy similarity1
Metal bindingi136IronBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Gluconeogenesis, Glycolysis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-11807.
BRENDAi5.3.1.9. 5243.
SABIO-RKP83194.
UniPathwayiUPA00109; UER00181.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate isomerase (EC:5.3.1.9)
Short name:
GPI
Alternative name(s):
Phosphoglucose isomerase
Short name:
PGI
Phosphohexose isomerase
Short name:
PHI
Gene namesi
Name:pgiA
Ordered Locus Names:PF0196
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001853571 – 189Glucose-6-phosphate isomeraseAdd BLAST189

Proteomic databases

PRIDEiP83194.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi186497.PF0196.

Structurei

Secondary structure

1189
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Turni12 – 14Combined sources3
Beta strandi22 – 26Combined sources5
Helixi27 – 30Combined sources4
Turni31 – 33Combined sources3
Helixi37 – 46Combined sources10
Beta strandi50 – 57Combined sources8
Beta strandi66 – 73Combined sources8
Beta strandi91 – 94Combined sources4
Beta strandi97 – 111Combined sources15
Beta strandi117 – 122Combined sources6
Beta strandi127 – 130Combined sources4
Beta strandi135 – 140Combined sources6
Beta strandi142 – 144Combined sources3
Beta strandi146 – 153Combined sources8
Helixi161 – 166Combined sources6
Beta strandi169 – 175Combined sources7
Beta strandi178 – 183Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QXJX-ray1.80A/B1-189[»]
1QXRX-ray1.70A/B1-189[»]
1QY4X-ray1.80A/B1-189[»]
1X7NX-ray1.89A1-189[»]
1X82X-ray1.50A1-189[»]
1X8EX-ray2.80A/B1-189[»]
2GC0X-ray2.00A/B1-187[»]
2GC1X-ray1.95A/B1-187[»]
2GC2X-ray2.10A/B1-187[»]
2GC3X-ray2.10A/B1-187[»]
3SXWX-ray1.80A1-189[»]
4LTAX-ray2.04A/B1-189[»]
4LUKX-ray1.41A1-189[»]
4LULX-ray1.89A/B1-189[»]
4LUMX-ray1.79A/B1-189[»]
ProteinModelPortaliP83194.
SMRiP83194.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP83194.

Family & Domainsi

Sequence similaritiesi

Belongs to the archaeal-type GPI family.Curated

Phylogenomic databases

eggNOGiarCOG02602. Archaea.
COG2140. LUCA.
HOGENOMiHOG000243903.
KOiK06859.
OMAiYPADAGH.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01410. G6P_isomerase_arch. 1 hit.
InterProiIPR016758. G6P_isomerase_archaea/bacteria.
IPR010551. G6P_isomerase_prok.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF06560. GPI. 1 hit.
[Graphical view]
PIRSFiPIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P83194-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYKEPFGVKV DFETGIIEGA KKSVRRLSDM EGYFVDERAW KELVEKEDPV
60 70 80 90 100
VYEVYAVEQE EKEGDLNFAT TVLYPGKVGK EFFFTKGHFH AKLDRAEVYV
110 120 130 140 150
ALKGKGGMLL QTPEGDAKWI SMEPGTVVYV PPYWAHRTVN IGDEPFIFLA
160 170 180
IYPADAGHDY GTIAEKGFSK IVIEENGEVK VVDNPRWKK
Length:189
Mass (Da):21,476
Last modified:March 1, 2002 - v1
Checksum:iC5D8380C59F2A785
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti11D → N AA sequence (PubMed:11533028).Curated1
Sequence conflicti28S → P AA sequence (PubMed:11344151).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF381250 Genomic DNA. Translation: AAL27992.1.
AE009950 Genomic DNA. Translation: AAL80320.1.
RefSeqiWP_011011309.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL80320; AAL80320; PF0196.
GeneIDi1468028.
KEGGipfu:PF0196.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF381250 Genomic DNA. Translation: AAL27992.1.
AE009950 Genomic DNA. Translation: AAL80320.1.
RefSeqiWP_011011309.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1QXJX-ray1.80A/B1-189[»]
1QXRX-ray1.70A/B1-189[»]
1QY4X-ray1.80A/B1-189[»]
1X7NX-ray1.89A1-189[»]
1X82X-ray1.50A1-189[»]
1X8EX-ray2.80A/B1-189[»]
2GC0X-ray2.00A/B1-187[»]
2GC1X-ray1.95A/B1-187[»]
2GC2X-ray2.10A/B1-187[»]
2GC3X-ray2.10A/B1-187[»]
3SXWX-ray1.80A1-189[»]
4LTAX-ray2.04A/B1-189[»]
4LUKX-ray1.41A1-189[»]
4LULX-ray1.89A/B1-189[»]
4LUMX-ray1.79A/B1-189[»]
ProteinModelPortaliP83194.
SMRiP83194.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF0196.

Proteomic databases

PRIDEiP83194.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL80320; AAL80320; PF0196.
GeneIDi1468028.
KEGGipfu:PF0196.

Phylogenomic databases

eggNOGiarCOG02602. Archaea.
COG2140. LUCA.
HOGENOMiHOG000243903.
KOiK06859.
OMAiYPADAGH.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00181.
BioCyciMetaCyc:MONOMER-11807.
BRENDAi5.3.1.9. 5243.
SABIO-RKP83194.

Miscellaneous databases

EvolutionaryTraceiP83194.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
HAMAPiMF_01410. G6P_isomerase_arch. 1 hit.
InterProiIPR016758. G6P_isomerase_archaea/bacteria.
IPR010551. G6P_isomerase_prok.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF06560. GPI. 1 hit.
[Graphical view]
PIRSFiPIRSF019325. Glucose-6-phosphate_isomerase. 1 hit.
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiG6PI_PYRFU
AccessioniPrimary (citable) accession number: P83194
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: March 1, 2002
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.