P83179 (MYRO2_BREBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Myrosinase 2 EC=3.2.1.147 Alternative name(s): Beta-glucosidase 2 Beta-thioglucosidase 2 Beta-thioglucosidase glucohydrolase 2 Myrosinase Sinigrinase 2 Thioglucosidase 2
Organism	Brevicoryne brassicae (Cabbage aphid)
Taxonomic identifier	69196 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Paraneoptera › Hemiptera › Sternorrhyncha › Aphidiformes › Aphidomorpha › Aphidoidea › Aphididae › Aphidinae › Macrosiphini › Brevicoryne

Protein attributes

Sequence length	140 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones By similarity. Ref.1 UniProtKB P37702
Catalytic activity	A thioglucoside + H2O = a sugar + a thiol. Ref.1
Enzyme regulation	Inhibited by ascorbate. Ref.1
Subunit structure	Homodimer. Ref.1
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.
Caution	The order of the peptides shown is unknown.
Biophysicochemical properties	Kinetic parameters: KM=0.41 mM for sinigrin (at pH 4.5 and 37 degrees Celsius) Ref.1 KM=0.52 mM for p-nitrophenyl-beta-glucopyranoside (at pH 4.5 and 37 degrees Celsius) Temperature dependence: Optimum temperature is about 40 degrees Celsius.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	thioglucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›140	›140	Myrosinase 2		PRO_0000063904
Sites
	Active site	70	1	Nucleophile By similarity UniProtKB P37702
Amino acid modifications
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	127	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-adjacent residues	12 – 13	2
	Non-adjacent residues	26 – 27	2
	Non-adjacent residues	35 – 36	2
	Non-adjacent residues	44 – 45	2
	Non-adjacent residues	58 – 59	2
	Non-adjacent residues	84 – 85	2
	Non-adjacent residues	97 – 98	2
	Non-adjacent residues	111 – 112	2
	Non-adjacent residues	124 – 125	2
	Non-terminal residue	1	1
	Non-terminal residue	140	1

Sequences

	Sequence		Length	Mass (Da)
	P83179 [UniParc]. Last modified March 23, 2010. Version 2. Checksum: A03EF5F737CD8ABD	FASTA	140	15,646
	10 20 30 40 50 60 DFMFGTSTAS GGLAPSGVMN SLEPKGLAYY NNLLNELLKV LFTYFGDRAY APNSPQRLSL 70 80 90 100 110 120 SLSGVFHLLR GTADFYALNH YSSRDKYGLP KLLLTEDGYG DDGQLDDFEK NYLNATLQAM 130 140 YLMKEQNVTS VHYTVNKCMN

References

[1]

"Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore." Pontoppidan B., Ekbom B., Eriksson S., Meijer J. Eur. J. Biochem. 268:1041-1048(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. Partial match. PS00653. GLYCOSYL_HYDROL_F1_2. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MYRO2_BREBR

Accession

Primary (citable) accession number: P83179

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	March 23, 2010
Last modified:	April 3, 2013
This is version 36 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)