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P83179

- MYRO2_BREBR

UniProt

P83179 - MYRO2_BREBR

Protein

Myrosinase 2

Gene
N/A
Organism
Brevicoryne brassicae (Cabbage aphid)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.By similarity

    Catalytic activityi

    A thioglucoside + H2O = a sugar + a thiol.1 Publication

    Enzyme regulationi

    Inhibited by ascorbate.1 Publication

    Kineticsi

    1. KM=0.41 mM for sinigrin (at pH 4.5 and 37 degrees Celsius)1 Publication
    2. KM=0.52 mM for p-nitrophenyl-beta-glucopyranoside (at pH 4.5 and 37 degrees Celsius)1 Publication

    Temperature dependencei

    Optimum temperature is about 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei70 – 701NucleophilePROSITE-ProRule annotation

    GO - Molecular functioni

    1. thioglucosidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase 2 (EC:3.2.1.147)
    Alternative name(s):
    Beta-glucosidase 2
    Beta-thioglucosidase 2
    Beta-thioglucosidase glucohydrolase 2
    Myrosinase
    Sinigrinase 2
    Thioglucosidase 2
    OrganismiBrevicoryne brassicae (Cabbage aphid)Curated
    Taxonomic identifieri69196 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidiformesAphidoideaAphididaeMacrosiphiniBrevicoryne

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›140›140Myrosinase 2PRO_0000063904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi127 – 1271N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Family and domain databases

    InterProiIPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Fragments.

    P83179-1 [UniParc]FASTAAdd to Basket

    « Hide

    DFMFGTSTAS GGLAPSGVMN SLEPKGLAYY NNLLNELLKV LFTYFGDRAY    50
    APNSPQRLSL SLSGVFHLLR GTADFYALNH YSSRDKYGLP KLLLTEDGYG 100
    DDGQLDDFEK NYLNATLQAM YLMKEQNVTS VHYTVNKCMN 140
    Length:140
    Mass (Da):15,646
    Last modified:March 23, 2010 - v2
    Checksum:iA03EF5F737CD8ABD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 111 Publication
    Non-adjacent residuesi12 – 1321 Publication
    Non-adjacent residuesi26 – 2721 Publication
    Non-adjacent residuesi35 – 3621 Publication
    Non-adjacent residuesi44 – 4521 Publication
    Non-adjacent residuesi58 – 5921 Publication
    Non-adjacent residuesi84 – 8521 Publication
    Non-adjacent residuesi97 – 9821 Publication
    Non-adjacent residuesi111 – 11221 Publication
    Non-adjacent residuesi124 – 12521 Publication
    Non-terminal residuei140 – 14011 Publication

    Cross-referencesi

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore."
      Pontoppidan B., Ekbom B., Eriksson S., Meijer J.
      Eur. J. Biochem. 268:1041-1048(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiMYRO2_BREBR
    AccessioniPrimary (citable) accession number: P83179
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: March 23, 2010
    Last modified: October 1, 2014
    This is version 39 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The order of the peptides shown is unknown.Curated

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3