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P83179

- MYRO2_BREBR

UniProt

P83179 - MYRO2_BREBR

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Protein
Myrosinase 2
Gene
N/A
Organism
Brevicoryne brassicae (Cabbage aphid)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones By similarity.By similarity1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.1 Publication

Enzyme regulationi

Inhibited by ascorbate.1 Publication

Kineticsi

  1. KM=0.41 mM for sinigrin (at pH 4.5 and 37 degrees Celsius)1 Publication
  2. KM=0.52 mM for p-nitrophenyl-beta-glucopyranoside (at pH 4.5 and 37 degrees Celsius)

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Nucleophile By similarityBy similarity

GO - Molecular functioni

  1. thioglucosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase 2 (EC:3.2.1.147)
    Alternative name(s):
    Beta-glucosidase 2
    Beta-thioglucosidase 2
    Beta-thioglucosidase glucohydrolase 2
    Myrosinase
    Sinigrinase 2
    Thioglucosidase 2
    OrganismiBrevicoryne brassicae (Cabbage aphid)
    Taxonomic identifieri69196 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidiformesAphidoideaAphididaeMacrosiphiniBrevicoryne

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini‹1 – ›140›140Myrosinase 2
    PRO_0000063904Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi114 – 1141N-linked (GlcNAc...) Reviewed prediction
    Glycosylationi127 – 1271N-linked (GlcNAc...) Reviewed prediction

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    InterProiIPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Fragments.

    P83179-1 [UniParc]FASTAAdd to Basket

    « Hide

    DFMFGTSTAS GGLAPSGVMN SLEPKGLAYY NNLLNELLKV LFTYFGDRAY    50
    APNSPQRLSL SLSGVFHLLR GTADFYALNH YSSRDKYGLP KLLLTEDGYG 100
    DDGQLDDFEK NYLNATLQAM YLMKEQNVTS VHYTVNKCMN 140
    Length:140
    Mass (Da):15,646
    Last modified:March 23, 2010 - v2
    Checksum:iA03EF5F737CD8ABD
    GO

    Non-adjacent residues

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-adjacent residuesi12 – 132
    Non-adjacent residuesi26 – 272
    Non-adjacent residuesi35 – 362
    Non-adjacent residuesi44 – 452
    Non-adjacent residuesi58 – 592
    Non-adjacent residuesi84 – 852
    Non-adjacent residuesi97 – 982
    Non-adjacent residuesi111 – 1122
    Non-adjacent residuesi124 – 1252

    Non-terminal residue

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11
    Non-terminal residuei140 – 1401

    Cross-referencesi

    3D structure databases

    ModBasei Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    InterProi IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore."
      Pontoppidan B., Ekbom B., Eriksson S., Meijer J.
      Eur. J. Biochem. 268:1041-1048(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

    Entry informationi

    Entry nameiMYRO2_BREBR
    AccessioniPrimary (citable) accession number: P83179
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: March 23, 2010
    Last modified: February 19, 2014
    This is version 38 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The order of the peptides shown is unknown.

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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