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P83179

- MYRO2_BREBR

UniProt

P83179 - MYRO2_BREBR

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Protein

Myrosinase 2

Gene
N/A
Organism
Brevicoryne brassicae (Cabbage aphid)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones By similarity.By similarity1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.1 Publication

Enzyme regulationi

Inhibited by ascorbate.1 Publication

Kineticsi

  1. KM=0.41 mM for sinigrin (at pH 4.5 and 37 degrees Celsius)1 Publication
  2. KM=0.52 mM for p-nitrophenyl-beta-glucopyranoside (at pH 4.5 and 37 degrees Celsius)

Temperature dependencei

Optimum temperature is about 40 degrees Celsius.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei70 – 701Nucleophile By similarityBy similarity

GO - Molecular functioni

  1. thioglucosidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Myrosinase 2 (EC:3.2.1.147)
Alternative name(s):
Beta-glucosidase 2
Beta-thioglucosidase 2
Beta-thioglucosidase glucohydrolase 2
Myrosinase
Sinigrinase 2
Thioglucosidase 2
OrganismiBrevicoryne brassicae (Cabbage aphid)
Taxonomic identifieri69196 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidiformesAphidoideaAphididaeMacrosiphiniBrevicoryne

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›140›140Myrosinase 2PRO_0000063904Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi114 – 1141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi127 – 1271N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

Homodimer.1 Publication

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiIPR017853. Glycoside_hydrolase_SF.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Fragments.

P83179-1 [UniParc]FASTAAdd to Basket

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DFMFGTSTAS GGLAPSGVMN SLEPKGLAYY NNLLNELLKV LFTYFGDRAY    50
APNSPQRLSL SLSGVFHLLR GTADFYALNH YSSRDKYGLP KLLLTEDGYG 100
DDGQLDDFEK NYLNATLQAM YLMKEQNVTS VHYTVNKCMN 140
Length:140
Mass (Da):15,646
Last modified:March 23, 2010 - v2
Checksum:iA03EF5F737CD8ABD
GO

Non-adjacent residues

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-adjacent residuesi12 – 132
Non-adjacent residuesi26 – 272
Non-adjacent residuesi35 – 362
Non-adjacent residuesi44 – 452
Non-adjacent residuesi58 – 592
Non-adjacent residuesi84 – 852
Non-adjacent residuesi97 – 982
Non-adjacent residuesi111 – 1122
Non-adjacent residuesi124 – 1252

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei140 – 1401

Cross-referencesi

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification and characterization of myrosinase from the cabbage aphid (Brevicoryne brassicae), a brassica herbivore."
    Pontoppidan B., Ekbom B., Eriksson S., Meijer J.
    Eur. J. Biochem. 268:1041-1048(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.

Entry informationi

Entry nameiMYRO2_BREBR
AccessioniPrimary (citable) accession number: P83179
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: March 23, 2010
Last modified: February 19, 2014
This is version 38 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Caution

The order of the peptides shown is unknown.

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3