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Protein

Myrosinase 2

Gene
N/A
Organism
Brevicoryne brassicae (Mealy cabbage aphid)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Degradation of glucosinolates (glucose residue linked by a thioglucoside bound to an amino acid derivative) to glucose, sulfate and any of the products: thiocyanates, isothiocyanates, nitriles, epithionitriles or oxazolidine-2-thiones.By similarity1 Publication

Catalytic activityi

A thioglucoside + H2O = a sugar + a thiol.1 Publication

Enzyme regulationi

Inhibited by ascorbate.1 Publication

Kineticsi

  1. KM=0.41 mM for sinigrin (at pH 4.5 and 37 degrees Celsius)1 Publication
  2. KM=0.52 mM for p-nitrophenyl-beta-glucopyranoside (at pH 4.5 and 37 degrees Celsius)1 Publication

    Temperature dependencei

    Optimum temperature is about 40 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei70NucleophilePROSITE-ProRule annotation1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosidase, Hydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Myrosinase 2 (EC:3.2.1.147)
    Alternative name(s):
    Beta-glucosidase 2
    Beta-thioglucosidase 2
    Beta-thioglucosidase glucohydrolase 2
    Myrosinase
    Sinigrinase 2
    Thioglucosidase 2
    OrganismiBrevicoryne brassicae (Mealy cabbage aphid)Curated
    Taxonomic identifieri69196 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraParaneopteraHemipteraSternorrhynchaAphidomorphaAphidoideaAphididaeMacrosiphiniBrevicoryne

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000063904‹1 – ›140Myrosinase 2Add BLAST›140

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi114N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi127N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Glycoprotein

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Structurei

    3D structure databases

    SMRiP83179.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 1 family.Curated

    Family and domain databases

    InterProiView protein in InterPro
    IPR017853. Glycoside_hydrolase_SF.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Fragments.

    P83179-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    DFMFGTSTAS GGLAPSGVMN SLEPKGLAYY NNLLNELLKV LFTYFGDRAY
    60 70 80 90 100
    APNSPQRLSL SLSGVFHLLR GTADFYALNH YSSRDKYGLP KLLLTEDGYG
    110 120 130 140
    DDGQLDDFEK NYLNATLQAM YLMKEQNVTS VHYTVNKCMN
    Length:140
    Mass (Da):15,646
    Last modified:March 23, 2010 - v2
    Checksum:iA03EF5F737CD8ABD
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Non-terminal residuei11 Publication1
    Non-adjacent residuesi12 – 131 Publication2
    Non-adjacent residuesi26 – 271 Publication2
    Non-adjacent residuesi35 – 361 Publication2
    Non-adjacent residuesi44 – 451 Publication2
    Non-adjacent residuesi58 – 591 Publication2
    Non-adjacent residuesi84 – 851 Publication2
    Non-adjacent residuesi97 – 981 Publication2
    Non-adjacent residuesi111 – 1121 Publication2
    Non-adjacent residuesi124 – 1251 Publication2
    Non-terminal residuei1401 Publication1

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiMYRO2_BREBR
    AccessioniPrimary (citable) accession number: P83179
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 17, 2003
    Last sequence update: March 23, 2010
    Last modified: May 10, 2017
    This is version 44 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Caution

    The order of the peptides shown is unknown.Curated

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families