ID   MTH12_DROME             Reviewed;         488 AA.
AC   P83119; Q8INH4;
DT   02-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2003, sequence version 2.
DT   27-MAR-2024, entry version 134.
DE   RecName: Full=Probable G-protein coupled receptor Mth-like 12;
DE   AltName: Full=Protein methuselah-like 12;
DE   Flags: Precursor;
GN   Name=mthl12; Synonyms=Mth-like-12; ORFNames=CG32853;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [3]
RP   IDENTIFICATION.
RX   PubMed=11274391; DOI=10.1073/pnas.051625298;
RA   West A.P. Jr., Llamas L.L., Snow P.M., Benzer S., Bjorkman P.J.;
RT   "Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-
RT   coupled receptor associated with extended lifespan.";
RL   Proc. Natl. Acad. Sci. U.S.A. 98:3744-3749(2001).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the G-protein coupled receptor 2 family. Mth
CC       subfamily. {ECO:0000305}.
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DR   EMBL; AE014297; AAN13556.2; -; Genomic_DNA.
DR   RefSeq; NP_731791.2; NM_169508.2.
DR   AlphaFoldDB; P83119; -.
DR   STRING; 7227.FBpp0082214; -.
DR   GlyCosmos; P83119; 5 sites, No reported glycans.
DR   GlyGen; P83119; 5 sites.
DR   PaxDb; 7227-FBpp0082214; -.
DR   EnsemblMetazoa; FBtr0082746; FBpp0082214; FBgn0045442.
DR   GeneID; 261599; -.
DR   KEGG; dme:Dmel_CG32853; -.
DR   AGR; FB:FBgn0045442; -.
DR   CTD; 261599; -.
DR   FlyBase; FBgn0045442; mthl12.
DR   VEuPathDB; VectorBase:FBgn0045442; -.
DR   eggNOG; KOG4193; Eukaryota.
DR   GeneTree; ENSGT00940000166745; -.
DR   HOGENOM; CLU_002753_3_0_1; -.
DR   InParanoid; P83119; -.
DR   OMA; FMEYFIW; -.
DR   OrthoDB; 3108840at2759; -.
DR   PhylomeDB; P83119; -.
DR   BioGRID-ORCS; 261599; 0 hits in 1 CRISPR screen.
DR   GenomeRNAi; 261599; -.
DR   PRO; PR:P83119; -.
DR   Proteomes; UP000000803; Chromosome 3R.
DR   GO; GO:0016020; C:membrane; ISS:FlyBase.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004930; F:G protein-coupled receptor activity; ISS:FlyBase.
DR   GO; GO:0007166; P:cell surface receptor signaling pathway; IEA:InterPro.
DR   GO; GO:0008340; P:determination of adult lifespan; ISS:UniProtKB.
DR   GO; GO:0007186; P:G protein-coupled receptor signaling pathway; ISS:FlyBase.
DR   GO; GO:0042594; P:response to starvation; ISS:UniProtKB.
DR   CDD; cd15039; 7tmB3_Methuselah-like; 1.
DR   Gene3D; 2.30.160.11; -; 1.
DR   Gene3D; 2.170.180.11; Methuselah ectodomain, domain 2; 1.
DR   Gene3D; 1.20.1070.10; Rhodopsin 7-helix transmembrane proteins; 1.
DR   InterPro; IPR022343; GCR1-cAMP_receptor.
DR   InterPro; IPR017981; GPCR_2-like_7TM.
DR   InterPro; IPR044860; Methusela_ecto_dom_1.
DR   InterPro; IPR023311; Methusela_ecto_dom_2.
DR   InterPro; IPR010596; Methuselah_N_dom.
DR   InterPro; IPR036272; Methuselah_N_sf.
DR   PANTHER; PTHR47154; G-PROTEIN COUPLED RECEPTOR MTH-RELATED; 1.
DR   PANTHER; PTHR47154:SF2; G-PROTEIN COUPLED RECEPTOR MTH-RELATED; 1.
DR   Pfam; PF06652; Methuselah_N; 1.
DR   PRINTS; PR02001; GCR1CAMPR.
DR   SUPFAM; SSF63877; Methuselah ectodomain; 1.
DR   PROSITE; PS50261; G_PROTEIN_RECEP_F2_4; 1.
DR   Genevisible; P83119; DM.
PE   3: Inferred from homology;
KW   Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW   Membrane; Receptor; Reference proteome; Signal; Transducer; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..488
FT                   /note="Probable G-protein coupled receptor Mth-like 12"
FT                   /id="PRO_0000013035"
FT   TOPO_DOM        18..215
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        237..247
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        248..268
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        269..283
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        284..304
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        305..315
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        316..336
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        337..373
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        374..394
FT                   /note="Helical; Name=5"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        395..416
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        417..437
FT                   /note="Helical; Name=6"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        438..454
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        455..475
FT                   /note="Helical; Name=7"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        476..488
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        19
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        34
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        55
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        141
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        365
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   DISULFID        27..81
FT                   /evidence="ECO:0000250|UniProtKB:O97148"
FT   DISULFID        83..88
FT                   /evidence="ECO:0000250|UniProtKB:O97148"
FT   DISULFID        92..189
FT                   /evidence="ECO:0000250|UniProtKB:O97148"
FT   DISULFID        93..104
FT                   /evidence="ECO:0000250|UniProtKB:O97148"
FT   DISULFID        155..209
FT                   /evidence="ECO:0000250|UniProtKB:O97148"
SQ   SEQUENCE   488 AA;  56781 MW;  3CE6B05F8D1DC103 CRC64;
     MFLWLKCFCT LIIVTIAKNS SAKIPHCKYD ETINISHFKR LNDAYIYEHF EIPANLTGEF
     DYKELMDGSK VPTEFPNLRG CICKVRPCIR ICCARKNILS NGECSDGVKN EIKLTMLDLT
     MQDILLTDPT LAELNMIPQY NSTELLILRE QFQPCDEIVS LKRDEYTILK DGSILLHTSA
     EILSNDQYCL YPEIYSDFPE TIRIINRRCY RNVMPGIAQL SVISVVGFIL TLAVYLSVEK
     LRNLLGKCLI CSLFSMFMEY FIWTMDYFRL LQSICSAAGY MKYFFSMSSY LWFSVVSFHL
     WELFTSLNRH EPQYRFLIYN TFVWCTAAIP TVVIFSMNQM WENDPGKSEW LPLVGYFGCS
     VKDWNSSSWF YSHIPIVILN SFNVIMFVLT AIYIWKVKKG VKSFAQHDER NTTCLEFNVQ
     TYIQFVRLFL IMGASWLLDQ LTRLAEDSHL LLDTIVLNLT VYLNAAFGIL IFVLLILKGS
     TFKMIMER
//