P83119 (MTH12_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable G-protein coupled receptor Mth-like 12

Alternative name(s):
Protein methuselah-like 12

Gene names

Name:	mthl12
Synonyms:	Mth-like-12
ORF Names:	CG32853

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the G-protein coupled receptor 2 family. Mth subfamily.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	determination of adult lifespan Inferred from sequence or structural similarity. Source: UniProtKB response to starvation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	G-protein coupled receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Chain	18 – 488	471	Probable G-protein coupled receptor Mth-like 12		PRO_0000013035
Regions
	Topological domain	18 – 215	198	Extracellular Potential
	Transmembrane	216 – 236	21	Helical; Name=1; Potential
	Topological domain	237 – 247	11	Cytoplasmic Potential
	Transmembrane	248 – 268	21	Helical; Name=2; Potential
	Topological domain	269 – 283	15	Extracellular Potential
	Transmembrane	284 – 304	21	Helical; Name=3; Potential
	Topological domain	305 – 315	11	Cytoplasmic Potential
	Transmembrane	316 – 336	21	Helical; Name=4; Potential
	Topological domain	337 – 373	37	Extracellular Potential
	Transmembrane	374 – 394	21	Helical; Name=5; Potential
	Topological domain	395 – 416	22	Cytoplasmic Potential
	Transmembrane	417 – 437	21	Helical; Name=6; Potential
	Topological domain	438 – 454	17	Extracellular Potential
	Transmembrane	455 – 475	21	Helical; Name=7; Potential
	Topological domain	476 – 488	13	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	19	1	N-linked (GlcNAc...) Potential
	Glycosylation	34	1	N-linked (GlcNAc...) Potential
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	141	1	N-linked (GlcNAc...) Potential
	Glycosylation	365	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 81		By similarity
	Disulfide bond	83 ↔ 88		By similarity
	Disulfide bond	92 ↔ 189		By similarity
	Disulfide bond	93 ↔ 104		By similarity
	Disulfide bond	155 ↔ 209		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83119 [UniParc]. Last modified July 11, 2003. Version 2. Checksum: 3CE6B05F8D1DC103	FASTA	488	56,781
	10 20 30 40 50 60 MFLWLKCFCT LIIVTIAKNS SAKIPHCKYD ETINISHFKR LNDAYIYEHF EIPANLTGEF 70 80 90 100 110 120 DYKELMDGSK VPTEFPNLRG CICKVRPCIR ICCARKNILS NGECSDGVKN EIKLTMLDLT 130 140 150 160 170 180 MQDILLTDPT LAELNMIPQY NSTELLILRE QFQPCDEIVS LKRDEYTILK DGSILLHTSA 190 200 210 220 230 240 EILSNDQYCL YPEIYSDFPE TIRIINRRCY RNVMPGIAQL SVISVVGFIL TLAVYLSVEK 250 260 270 280 290 300 LRNLLGKCLI CSLFSMFMEY FIWTMDYFRL LQSICSAAGY MKYFFSMSSY LWFSVVSFHL 310 320 330 340 350 360 WELFTSLNRH EPQYRFLIYN TFVWCTAAIP TVVIFSMNQM WENDPGKSEW LPLVGYFGCS 370 380 390 400 410 420 VKDWNSSSWF YSHIPIVILN SFNVIMFVLT AIYIWKVKKG VKSFAQHDER NTTCLEFNVQ 430 440 450 460 470 480 TYIQFVRLFL IMGASWLLDQ LTRLAEDSHL LLDTIVLNLT VYLNAAFGIL IFVLLILKGS TFKMIMER

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"Crystal structure of the ectodomain of Methuselah, a Drosophila G protein-coupled receptor associated with extended lifespan." West A.P. Jr., Llamas L.L., Snow P.M., Benzer S., Bjorkman P.J. Proc. Natl. Acad. Sci. U.S.A. 98:3744-3749(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAN13556.2.
RefSeq	NP_731791.2. NM_169508.2.
UniGene	Dm.26413.
3D structure databases
ProteinModelPortal	P83119.
ModBase	Search...
Protein-protein interaction databases
STRING	7227.FBpp0082214.
Protein family/group databases
GPCRDB	Search...
Proteomic databases
PaxDb	P83119.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0082746; FBpp0082214; FBgn0045442.
GeneID	261599.
KEGG	dme:Dmel_CG32853.
Organism-specific databases
CTD	261599.
FlyBase	FBgn0045442. mthl12.
Phylogenomic databases
eggNOG	KOG4193.
GeneTree	ENSGT00530000064566.
InParanoid	P83119.
OrthoDB	EOG4Z613V.
PhylomeDB	P83119.
Gene expression databases
GermOnline	CG32853. Drosophila melanogaster.
Family and domain databases
Gene3D	2.170.180.11. 1 hit.
InterPro	IPR022343. GCR1-cAMP_receptor. IPR017981. GPCR_2-like. IPR023311. Methusela_ecto_dom_2. IPR010596. Methuselah_N_dom. [Graphical view]
Pfam	PF06652. Methuselah_N. 1 hit. [Graphical view]
PRINTS	PR02001. GCR1CAMPR.
PROSITE	PS00649. G_PROTEIN_RECEP_F2_1. False negative. PS00650. G_PROTEIN_RECEP_F2_2. False negative. PS50261. G_PROTEIN_RECEP_F2_4. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	261599.
NextBio	843695.

Entry information

Entry name

MTH12_DROME

Accession

Primary (citable) accession number: P83119
Secondary accession number(s): Q8INH4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	July 11, 2003
Last modified:	May 1, 2013
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project