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P83111 (LACTB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine beta-lactamase-like protein LACTB, mitochondrial

EC=3.4.-.-
Gene names
Name:LACTB
Synonyms:MRPL56
ORF Names:UNQ843/PRO1781
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length547 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Mitochondrion Ref.4 Ref.6.

Tissue specificity

Expressed predominantly in skeletal muscle.

Sequence similarities

Belongs to the peptidase S12 family.

Caution

Was originally (Ref.4) identified as 39S ribosomal protein L56 (MRP-L56).

Ontologies

Keywords
   Cellular componentMitochondrion
   Coding sequence diversityAlternative splicing
Polymorphism
   DomainTransit peptide
   Molecular functionHydrolase
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from direct assay. Source: HPA

mitochondrion

Inferred from direct assay PubMed 20833797. Source: UniProt

   Molecular_functionhydrolase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P83111-1)

Also known as: a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P83111-2)

The sequence of this isoform differs from the canonical sequence as follows:
     374-547: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 115115Mitochondrion Potential
Chain116 – 547432Serine beta-lactamase-like protein LACTB, mitochondrial
PRO_0000195476

Sites

Active site1641Acyl-ester intermediate By similarity

Amino acid modifications

Modified residue2831N6-succinyllysine By similarity
Modified residue2841N6-succinyllysine By similarity
Modified residue2971N6-acetyllysine By similarity
Modified residue3421N6-acetyllysine Ref.7

Natural variations

Alternative sequence374 – 547174Missing in isoform 2.
VSP_010011
Natural variant4691R → K.
Corresponds to variant rs2729835 [ dbSNP | Ensembl ].
VAR_018299

Experimental info

Sequence conflict51M → L Ref.1
Sequence conflict51M → L Ref.2
Sequence conflict611R → S in BAB55384. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (a) [UniParc].

Last modified April 13, 2004. Version 2.
Checksum: 4CFAF56D2A390264

FASTA54760,694
        10         20         30         40         50         60 
MYRLMSAVTA RAAAPGGLAS SCGRRGVHQR AGLPPLGHGW VGGLGLGLGL ALGVKLAGGL 

        70         80         90        100        110        120 
RGAAPAQSPA APDPEASPLA EPPQEQSLAP WSPQTPAPPC SRCFARAIES SRDLLHRIKD 

       130        140        150        160        170        180 
EVGAPGIVVG VSVDGKEVWS EGLGYADVEN RVPCKPETVM RIASISKSLT MVALAKLWEA 

       190        200        210        220        230        240 
GKLDLDIPVQ HYVPEFPEKE YEGEKVSVTT RLLISHLSGI RHYEKDIKKV KEEKAYKALK 

       250        260        270        280        290        300 
MMKENVAFEQ EKEGKSNEKN DFTKFKTEQE NEAKCRNSKP GKKKNDFEQG ELYLREKFEN 

       310        320        330        340        350        360 
SIESLRLFKN DPLFFKPGSQ FLYSTFGYTL LAAIVERASG CKYLDYMQKI FHDLDMLTTV 

       370        380        390        400        410        420 
QEENEPVIYN RARFYVYNKK KRLVNTPYVD NSYKWAGGGF LSTVGDLLKF GNAMLYGYQV 

       430        440        450        460        470        480 
GLFKNSNENL LPGYLKPETM VMMWTPVPNT EMSWDKEGKY AMAWGVVERK QTYGSCRKQR 

       490        500        510        520        530        540 
HYASHTGGAV GASSVLLVLP EELDTETINN KVPPRGIIVS IICNMQSVGL NSTALKIALE 


FDKDRSD 

« Hide

Isoform 2 [UniParc].

Checksum: CD28AA12A2F1C74A
Show »

FASTA37341,239

References

« Hide 'large scale' references
[1]"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. expand/collapse author list , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.
Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Placenta.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Pituitary.
[4]"The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present."
Koc E.C., Burkhart W., Blackburn K., Moyer M.B., Schlatzer D.M., Moseley A., Spremulli L.L.
J. Biol. Chem. 276:43958-43969(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, CONCEPTUAL TRANSLATION.
[5]"Identification, genomic organization, and mRNA expression of LACTB, encoding a serine beta-lactamase-like protein with an amino-terminal transmembrane domain."
Smith T.S., Southan C., Ellington K., Campbell D., Tew D.G., Debouck C.
Genomics 78:12-14(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, CONCEPTUAL TRANSLATION.
[6]"LACTB is a filament-forming protein localized in mitochondria."
Polianskyte Z., Peitsaro N., Dapkunas A., Liobikas J., Soliymani R., Lalowski M., Speer O., Seitsonen J., Butcher S., Cereghetti G.M., Linder M.D., Merckel M., Thompson J., Eriksson O.
Proc. Natl. Acad. Sci. U.S.A. 106:18960-18965(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[7]"Lysine acetylation targets protein complexes and co-regulates major cellular functions."
Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M.
Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-342, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AK027808 mRNA. Translation: BAB55384.1.
AY358709 mRNA. Translation: AAQ89072.1.
BC067288 mRNA. Translation: AAH67288.1.
RefSeqNP_001275514.1. NM_001288585.1.
NP_116246.2. NM_032857.4.
NP_741982.1. NM_171846.3.
UniGeneHs.410388.

3D structure databases

ProteinModelPortalP83111.
SMRP83111. Positions 108-542.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid125311. 5 interactions.
IntActP83111. 2 interactions.
MINTMINT-2804983.
STRING9606.ENSP00000261893.

Chemistry

BindingDBP83111.

Protein family/group databases

MEROPSS12.004.

PTM databases

PhosphoSiteP83111.

Polymorphism databases

DMDM46397478.

Proteomic databases

PaxDbP83111.
PRIDEP83111.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261893; ENSP00000261893; ENSG00000103642. [P83111-1]
ENST00000413507; ENSP00000392956; ENSG00000103642. [P83111-2]
GeneID114294.
KEGGhsa:114294.
UCSCuc002alv.3. human. [P83111-1]

Organism-specific databases

CTD114294.
GeneCardsGC15P063413.
HGNCHGNC:16468. LACTB.
HPAHPA036361.
HPA036362.
MIM608440. gene.
neXtProtNX_P83111.
PharmGKBPA30262.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1680.
HOGENOMHOG000060285.
HOVERGENHBG052296.
InParanoidP83111.
KOK17382.
OMAQVGLFKN.
OrthoDBEOG7353WG.
PhylomeDBP83111.
TreeFamTF315050.

Gene expression databases

ArrayExpressP83111.
BgeeP83111.
CleanExHS_LACTB.
GenevestigatorP83111.

Family and domain databases

Gene3D3.40.710.10. 2 hits.
InterProIPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
[Graphical view]
PfamPF00144. Beta-lactamase. 2 hits.
[Graphical view]
SUPFAMSSF56601. SSF56601. 2 hits.
ProtoNetSearch...

Other

GeneWikiLACTB.
GenomeRNAi114294.
NextBio79053.
PROP83111.
SOURCESearch...

Entry information

Entry nameLACTB_HUMAN
AccessionPrimary (citable) accession number: P83111
Secondary accession number(s): P83096
Entry history
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: April 13, 2004
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM