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Protein

Serine beta-lactamase-like protein LACTB, mitochondrial

Gene

LACTB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Mitochondrial serine protease that acts as a regulator of mitochondrial lipid metabolism (PubMed:28329758). Acts by decreasing protein levels of PISD, a mitochondrial enzyme that converts phosphatidylserine (PtdSer) to phosphatidylethanolamine (PtdEtn), thereby affecting mitochondrial lipid metabolism (PubMed:28329758). It is unclear whether it acts directly by mediating proteolysis of PISD or by mediating proteolysis of another lipid metabolism protein (PubMed:28329758). Acts as a tumor suppressor that has the ability to inhibit proliferation of multiple types of breast cancer cells: probably by promoting decreased levels of PISD, thereby affecting mitochondrial lipid metabolism (PubMed:28329758).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei164Acyl-ester intermediateBy similarity1

GO - Molecular functioni

  • peptidase activity Source: UniProtKB

GO - Biological processi

  • lipid metabolic process Source: UniProtKB-KW
  • proteolysis Source: UniProtKB
  • regulation of lipid metabolic process Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease
Biological processLipid metabolism

Protein family/group databases

MEROPSiS12.004.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine beta-lactamase-like protein LACTB, mitochondrialCurated (EC:3.4.-.-1 Publication)
Gene namesi
Name:LACTBImported
Synonyms:MRPL561 Publication
ORF Names:UNQ843/PRO17811 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:16468. LACTB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HPA
  • mitochondrion Source: UniProtKB

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi114294.
OpenTargetsiENSG00000103642.
PharmGKBiPA30262.

Polymorphism and mutation databases

BioMutaiLACTB.
DMDMi46397478.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 115MitochondrionSequence analysisAdd BLAST115
ChainiPRO_0000195476116 – 547Serine beta-lactamase-like protein LACTB, mitochondrialAdd BLAST432

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei283N6-succinyllysineBy similarity1
Modified residuei284N6-succinyllysineBy similarity1
Modified residuei297N6-acetyllysineBy similarity1
Modified residuei342N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP83111.
MaxQBiP83111.
PaxDbiP83111.
PeptideAtlasiP83111.
PRIDEiP83111.

PTM databases

iPTMnetiP83111.
PhosphoSitePlusiP83111.

Expressioni

Tissue specificityi

Expressed predominantly in skeletal muscle.1 Publication

Inductioni

Down-regulated in a number of cancer cells.1 Publication

Gene expression databases

BgeeiENSG00000103642.
CleanExiHS_LACTB.
ExpressionAtlasiP83111. baseline and differential.
GenevisibleiP83111. HS.

Organism-specific databases

HPAiHPA036361.
HPA036362.

Interactioni

Protein-protein interaction databases

BioGridi125311. 22 interactors.
IntActiP83111. 9 interactors.
MINTiMINT-2804983.
STRINGi9606.ENSP00000261893.

Structurei

3D structure databases

ProteinModelPortaliP83111.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S12 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHHD. Eukaryota.
COG1680. LUCA.
GeneTreeiENSGT00390000001062.
HOGENOMiHOG000060285.
HOVERGENiHBG052296.
InParanoidiP83111.
KOiK17382.
OMAiTFLYSTH.
OrthoDBiEOG091G0BW6.
PhylomeDBiP83111.
TreeFamiTF315050.

Family and domain databases

InterProiView protein in InterPro
IPR001466. Beta-lactam-related.
IPR012338. Beta-lactam/transpept-like.
PfamiView protein in Pfam
PF00144. Beta-lactamase. 2 hits.
SUPFAMiSSF56601. SSF56601. 2 hits.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P83111-1) [UniParc]FASTAAdd to basket
Also known as: a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYRLMSAVTA RAAAPGGLAS SCGRRGVHQR AGLPPLGHGW VGGLGLGLGL
60 70 80 90 100
ALGVKLAGGL RGAAPAQSPA APDPEASPLA EPPQEQSLAP WSPQTPAPPC
110 120 130 140 150
SRCFARAIES SRDLLHRIKD EVGAPGIVVG VSVDGKEVWS EGLGYADVEN
160 170 180 190 200
RVPCKPETVM RIASISKSLT MVALAKLWEA GKLDLDIPVQ HYVPEFPEKE
210 220 230 240 250
YEGEKVSVTT RLLISHLSGI RHYEKDIKKV KEEKAYKALK MMKENVAFEQ
260 270 280 290 300
EKEGKSNEKN DFTKFKTEQE NEAKCRNSKP GKKKNDFEQG ELYLREKFEN
310 320 330 340 350
SIESLRLFKN DPLFFKPGSQ FLYSTFGYTL LAAIVERASG CKYLDYMQKI
360 370 380 390 400
FHDLDMLTTV QEENEPVIYN RARFYVYNKK KRLVNTPYVD NSYKWAGGGF
410 420 430 440 450
LSTVGDLLKF GNAMLYGYQV GLFKNSNENL LPGYLKPETM VMMWTPVPNT
460 470 480 490 500
EMSWDKEGKY AMAWGVVERK QTYGSCRKQR HYASHTGGAV GASSVLLVLP
510 520 530 540
EELDTETINN KVPPRGIIVS IICNMQSVGL NSTALKIALE FDKDRSD
Length:547
Mass (Da):60,694
Last modified:April 13, 2004 - v2
Checksum:i4CFAF56D2A390264
GO
Isoform 2 (identifier: P83111-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     374-547: Missing.

Show »
Length:373
Mass (Da):41,239
Checksum:iCD28AA12A2F1C74A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti61R → S in BAB55384 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018299469R → K Common polymorphism; does not affect serine protease activity; shows reduced tumor suppressor activity; shows reduced ability to downregulate phosphatidylethanolamine (PtdEtn) levels. 1 PublicationCorresponds to variant dbSNP:rs2729835Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_010011374 – 547Missing in isoform 2. 2 PublicationsAdd BLAST174

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK027808 mRNA. Translation: BAB55384.1.
AY358709 mRNA. Translation: AAQ89072.1.
BC067288 mRNA. Translation: AAH67288.1.
CCDSiCCDS10182.1. [P83111-1]
CCDS45275.1. [P83111-2]
RefSeqiNP_001275514.1. NM_001288585.1.
NP_116246.2. NM_032857.4. [P83111-1]
NP_741982.1. NM_171846.3. [P83111-2]
UniGeneiHs.410388.

Genome annotation databases

EnsembliENST00000261893; ENSP00000261893; ENSG00000103642. [P83111-1]
ENST00000413507; ENSP00000392956; ENSG00000103642. [P83111-2]
GeneIDi114294.
KEGGihsa:114294.
UCSCiuc002alv.5. human. [P83111-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiLACTB_HUMAN
AccessioniPrimary (citable) accession number: P83111
Secondary accession number(s): P83096
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: April 13, 2004
Last modified: August 30, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally identified as mitochondrial large ribosomal subunit protein mL56 (MRP-L56) (PubMed:11551941), but has since been shown to localize to the mitochondrial intermembrane space where it forms filaments (PubMed:19858488).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Peptidase families
    Classification of peptidase families and list of entries
  6. SIMILARITY comments
    Index of protein domains and families