P83111 (LACTB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine beta-lactamase-like protein LACTB, mitochondrial
EC=3.4.-.-

Gene names

Name:	LACTB
Synonyms:	MRPL56
ORF Names:	UNQ843/PRO1781

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	547 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion Ref.4 Ref.6.
Tissue specificity	Expressed predominantly in skeletal muscle.
Sequence similarities	Belongs to the peptidase S12 family.
Caution	Was originally (Ref.4) identified as 39S ribosomal protein L56 (MRP-L56).

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transit peptide
Molecular function	Hydrolase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from direct assay. Source: HPA
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P83111-1)

Also known as: a;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P83111-2)

The sequence of this isoform differs from the canonical sequence as follows:
     374-547: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 115	115	Mitochondrion Potential
	Chain	116 – 547	432	Serine beta-lactamase-like protein LACTB, mitochondrial		PRO_0000195476
Sites
	Active site	164	1	Acyl-ester intermediate By similarity
Amino acid modifications
	Modified residue	164	1	Phosphoserine By similarity
	Modified residue	342	1	N6-acetyllysine Ref.7
	Modified residue	459	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	374 – 547	174	Missing in isoform 2.		VSP_010011
	Natural variant	469	1	R → K. Corresponds to variant rs2729835 [ dbSNP | Ensembl ].		VAR_018299
Experimental info
	Sequence conflict	5	1	M → L Ref.1
	Sequence conflict	5	1	M → L Ref.2
	Sequence conflict	61	1	R → S in BAB55384. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (a) [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 4CFAF56D2A390264	FASTA	547	60,694
	10 20 30 40 50 60 MYRLMSAVTA RAAAPGGLAS SCGRRGVHQR AGLPPLGHGW VGGLGLGLGL ALGVKLAGGL 70 80 90 100 110 120 RGAAPAQSPA APDPEASPLA EPPQEQSLAP WSPQTPAPPC SRCFARAIES SRDLLHRIKD 130 140 150 160 170 180 EVGAPGIVVG VSVDGKEVWS EGLGYADVEN RVPCKPETVM RIASISKSLT MVALAKLWEA 190 200 210 220 230 240 GKLDLDIPVQ HYVPEFPEKE YEGEKVSVTT RLLISHLSGI RHYEKDIKKV KEEKAYKALK 250 260 270 280 290 300 MMKENVAFEQ EKEGKSNEKN DFTKFKTEQE NEAKCRNSKP GKKKNDFEQG ELYLREKFEN 310 320 330 340 350 360 SIESLRLFKN DPLFFKPGSQ FLYSTFGYTL LAAIVERASG CKYLDYMQKI FHDLDMLTTV 370 380 390 400 410 420 QEENEPVIYN RARFYVYNKK KRLVNTPYVD NSYKWAGGGF LSTVGDLLKF GNAMLYGYQV 430 440 450 460 470 480 GLFKNSNENL LPGYLKPETM VMMWTPVPNT EMSWDKEGKY AMAWGVVERK QTYGSCRKQR 490 500 510 520 530 540 HYASHTGGAV GASSVLLVLP EELDTETINN KVPPRGIIVS IICNMQSVGL NSTALKIALE FDKDRSD
	Isoform 2 [UniParc]. Checksum: CD28AA12A2F1C74A	FASTA	373	41,239
	10 20 30 40 50 60 MYRLMSAVTA RAAAPGGLAS SCGRRGVHQR AGLPPLGHGW VGGLGLGLGL ALGVKLAGGL 70 80 90 100 110 120 RGAAPAQSPA APDPEASPLA EPPQEQSLAP WSPQTPAPPC SRCFARAIES SRDLLHRIKD 130 140 150 160 170 180 EVGAPGIVVG VSVDGKEVWS EGLGYADVEN RVPCKPETVM RIASISKSLT MVALAKLWEA 190 200 210 220 230 240 GKLDLDIPVQ HYVPEFPEKE YEGEKVSVTT RLLISHLSGI RHYEKDIKKV KEEKAYKALK 250 260 270 280 290 300 MMKENVAFEQ EKEGKSNEKN DFTKFKTEQE NEAKCRNSKP GKKKNDFEQG ELYLREKFEN 310 320 330 340 350 360 SIESLRLFKN DPLFFKPGSQ FLYSTFGYTL LAAIVERASG CKYLDYMQKI FHDLDMLTTV 370 QEENEPVIYN RAR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Pituitary.
[4]	"The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present." Koc E.C., Burkhart W., Blackburn K., Moyer M.B., Schlatzer D.M., Moseley A., Spremulli L.L. J. Biol. Chem. 276:43958-43969(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, SUBCELLULAR LOCATION, CONCEPTUAL TRANSLATION.
[5]	"Identification, genomic organization, and mRNA expression of LACTB, encoding a serine beta-lactamase-like protein with an amino-terminal transmembrane domain." Smith T.S., Southan C., Ellington K., Campbell D., Tew D.G., Debouck C. Genomics 78:12-14(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, CONCEPTUAL TRANSLATION.
[6]	"LACTB is a filament-forming protein localized in mitochondria." Polianskyte Z., Peitsaro N., Dapkunas A., Liobikas J., Soliymani R., Lalowski M., Speer O., Seitsonen J., Butcher S., Cereghetti G.M., Linder M.D., Merckel M., Thompson J., Eriksson O. Proc. Natl. Acad. Sci. U.S.A. 106:18960-18965(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
[7]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T.C., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-342, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK027808 mRNA. Translation: BAB55384.1. AY358709 mRNA. Translation: AAQ89072.1. BC067288 mRNA. Translation: AAH67288.1.
IPI	IPI00294186. IPI00375490.
RefSeq	NP_116246.2. NM_032857.3. NP_741982.1. NM_171846.2.
UniGene	Hs.410388.
3D structure databases
ProteinModelPortal	P83111.
ModBase	Search...
Protein-protein interaction databases
IntAct	P83111. 2 interactions.
STRING	9606.ENSP00000261893.
Protein family/group databases
MEROPS	S12.004.
PTM databases
PhosphoSite	P83111.
Polymorphism databases
DMDM	46397478.
Proteomic databases
PaxDb	P83111.
PRIDE	P83111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000261893; ENSP00000261893; ENSG00000103642. ENST00000413507; ENSP00000392956; ENSG00000103642.
GeneID	114294.
KEGG	hsa:114294.
UCSC	uc002alv.3. human.
Organism-specific databases
CTD	114294.
GeneCards	GC15P063413.
HGNC	HGNC:16468. LACTB.
HPA	HPA036361. HPA036362.
MIM	608440. gene.
neXtProt	NX_P83111.
PharmGKB	PA30262.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG1680.
HOGENOM	HOG000060285.
HOVERGEN	HBG052296.
InParanoid	P83111.
OMA	MWTPVPN.
OrthoDB	EOG4GQQ58.
Gene expression databases
ArrayExpress	P83111.
Bgee	P83111.
CleanEx	HS_LACTB.
Genevestigator	P83111.
GermOnline	ENSG00000103642. Homo sapiens.
Family and domain databases
Gene3D	3.40.710.10. 2 hits.
InterPro	IPR001466. Beta-lactam-related. IPR012338. Beta-lactam/transpept-like. [Graphical view]
Pfam	PF00144. Beta-lactamase. 2 hits. [Graphical view]
SUPFAM	SSF56601. PBP_transp_fold. 1 hit.
ProtoNet	Search...
Other
BindingDB	P83111.
ChEMBL	CHEMBL3791.
GenomeRNAi	114294.
NextBio	79053.
SOURCE	Search...

Entry information

Entry name

LACTB_HUMAN

Accession

Primary (citable) accession number: P83111
Secondary accession number(s): P83096

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	April 13, 2004
Last modified:	May 1, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.