P83101 (GSK3H_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative glycogen synthase kinase-3 homolog
Short name=GSK-3
EC=2.7.11.26

Alternative name(s):
Protein gasket

Gene names

Name:	gskt
ORF Names:	CG31003

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + [tau protein] = ADP + [tau protein] phosphate.
Post-translational modification	Phosphorylation on Tyr-193 is necessary for the activity By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	male gamete generation Inferred from mutant phenotype PubMed 17179138. Source: FlyBase
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Non-traceable author statement PubMed 10908587. Source: FlyBase tau-protein kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Putative glycogen synthase kinase-3 homolog		PRO_0000085984
Regions
	Domain	33 – 317	285	Protein kinase
	Nucleotide binding	39 – 47	9	ATP By similarity
	Compositional bias	452 – 499	48	Asp-rich
Sites
	Active site	158	1	Proton acceptor By similarity
	Binding site	62	1	ATP By similarity
Amino acid modifications
	Modified residue	193	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P83101 [UniParc]. Last modified October 1, 2001. Version 1. Checksum: 949DBCADBD72A2C9	FASTA	501	56,237
	10 20 30 40 50 60 MASQSKNSGL TNKVTTVVAT NAFGADVMSE ISYTDAKVVG NGSFGVVFQA KMVPSNEMVA 70 80 90 100 110 120 IKKVLQDRRF KNRELQIMRK LRHDNIITLK WFFFSSGEKR DEVYLNLVME FLPETLYKVE 130 140 150 160 170 180 RQYARAKQTL PVNFVRLYMY QLLRSMGYLH SLGFCHRDIK PQNMLLDSET GVLKLCDFGS 190 200 210 220 230 240 AKQLISGEPN VSYICSRYYR APELIFGSTD YTTKIDMWSA GCVMSELLLG QLIFPGDSGV 250 260 270 280 290 300 DQIVEIVKVM GTPTSEQLHD MNPHYKQFKL PELKPHPWSK VFRIRTPAEA IDLVSKMLIY 310 320 330 340 350 360 SPNARVSPLM GCAHPFFDEL RQDPHQQLPN GRSLPPLFNF TDYEKTIEPD TMPLLLPRAQ 370 380 390 400 410 420 GSSTTKEPSA AHRNRNTAGE ESPRKTEDSQ KPATAALSKS PGPSGKALES PPGFLQHDLG 430 440 450 460 470 480 NGDHVAVGTM PMEPLTLEQN HFAAESYAVG EDAEDNLEED VGDENDYDYD DGGQCNSTYI 490 500 SDDMDEASES DDDDFEEEDE N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[2]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[3]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAN14270.1. BT001338 mRNA. Translation: AAN71093.1.
RefSeq	NP_733426.1. NM_170547.3.
UniGene	Dm.14965.
3D structure databases
ProteinModelPortal	P83101.
SMR	P83101. Positions 12-496.
ModBase	Search...
Protein-protein interaction databases
IntAct	P83101. 1 interaction.
STRING	7227.FBpp0085145.
Proteomic databases
PRIDE	P83101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0085784; FBpp0085145; FBgn0046332.
GeneID	318552.
KEGG	dme:Dmel_CG31003.
UCSC	CG31003-RA. d. melanogaster.
Organism-specific databases
CTD	318552.
FlyBase	FBgn0046332. gskt.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00520000055635.
InParanoid	P83101.
KO	K03083.
OMA	THEVKIC.
OrthoDB	EOG4H70SQ.
PhylomeDB	P83101.
Gene expression databases
Bgee	P83101.
GermOnline	CG31003. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	318552.
NextBio	845394.

Entry information

Entry name

GSK3H_DROME

Accession

Primary (citable) accession number: P83101

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 2, 2001
Last sequence update:	October 1, 2001
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project