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Protein

Stromal interaction molecule 2

Gene

Stim2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in mediating store-operated Ca2+ entry (SOCE), a Ca2+ influx following depletion of intracellular Ca2+ stores. Functions as a highly sensitive Ca2+ sensor in the endoplasmic reticulum which activates both store-operated and store-independent Ca2+-influx. Regulates basal cytosolic and endoplasmic reticulum Ca2+ concentrations. Upon mild variations of the endoplasmic reticulum Ca2+ concentration, translocates from the endoplasmic reticulum to the plasma membrane where it probably activates the Ca2+ release-activated Ca2+ (CRAC) channels ORAI1, ORAI2 and ORAI3. May inhibit STIM1-mediated Ca2+ influx (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi80 – 9112By similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal interaction molecule 2
Gene namesi
Name:Stim2
Synonyms:Kiaa1482
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:2151156. Stim2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini15 – 218204ExtracellularSequence analysisAdd
BLAST
Transmembranei219 – 23517HelicalSequence analysisAdd
BLAST
Topological domaini236 – 746511CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1414Sequence analysisAdd
BLAST
Chaini15 – 746732Stromal interaction molecule 2PRO_0000218282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281PhosphoserineCombined sources
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence analysis
Modified residuei523 – 5231PhosphoserineCombined sources
Modified residuei609 – 6091PhosphoserineBy similarity
Modified residuei621 – 6211PhosphoserineBy similarity
Modified residuei640 – 6401PhosphoserineCombined sources
Modified residuei650 – 6501PhosphoserineCombined sources
Modified residuei661 – 6611PhosphoserineBy similarity
Modified residuei680 – 6801PhosphoserineBy similarity
Modified residuei697 – 6971PhosphoserineBy similarity

Post-translational modificationi

Glycosylated.By similarity
Phosphorylated predominantly on Ser residues.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP83093.
MaxQBiP83093.
PaxDbiP83093.
PRIDEiP83093.

PTM databases

iPTMnetiP83093.

Expressioni

Gene expression databases

BgeeiP83093.
CleanExiMM_STIM2.
ExpressionAtlasiP83093. baseline and differential.
GenevisibleiP83093. MM.

Interactioni

Subunit structurei

Oligomer with STIM1. Interacts with ORAI1 (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113174.

Structurei

3D structure databases

ProteinModelPortaliP83093.
SMRiP83093. Positions 62-205, 251-448.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini67 – 10236EF-handAdd
BLAST
Domaini136 – 20469SAMPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili247 – 394148Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi534 – 55219His-richAdd
BLAST

Sequence similaritiesi

Contains 1 EF-hand domain.Curated
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
GeneTreeiENSGT00390000000214.
HOGENOMiHOG000261647.
HOVERGENiHBG079219.
InParanoidiP83093.
KOiK18196.
OMAiGTMAKPA.
OrthoDBiEOG7XSTDR.
PhylomeDBiP83093.
TreeFamiTF313487.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83093-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLFGLLVAG VADGCDLVPR HLRGRRASGS AGAAASPSAA AAGERQALLT
60 70 80 90 100
DPCMSLSPPC FTEEDRFSLE ALQTIHKQMD DDKDGGIEVD ESDEFIREDM
110 120 130 140 150
KYKDATNKHS HLHREDKHIT VEDLWKQWKT SEVHNWTLED TLQWLIEFVE
160 170 180 190 200
LPQYEKNFRD NNVKGTTLPR IAVHETSFMI SQLKISDRSH RQKLQLKALD
210 220 230 240 250
VVLFGPLTRP PHNWMKDFIL TISIVIGVGG CWFAYTQNKT SKEHVAKMMK
260 270 280 290 300
DLESLQTAEQ SLMDLQERLE KAQEENRTVA VEKQNLERKM MDEINYAKEE
310 320 330 340 350
ACRLRELREG AECELSRRQY AEQELEQVRM ALKKAEKEFE LRSSWSVPDA
360 370 380 390 400
LQKWLQLTHE VEVQYYNIKR QNAEMQLAIA KDEAEKIKKK RSTVFGTLHV
410 420 430 440 450
AHSSSLDEVD HKILEAKKAL SELTTCLRER LFRWQQIEKI CGFQIAHNSG
460 470 480 490 500
LPSLTSSLYS DHSWVVMPRV SIPPYPIAGG VDDLDEDTPP IVPQFPGTVA
510 520 530 540 550
KPAGSLARSS SLCRSRRSIV PSSPQSQRAQ LPAHAPLAAH PRHPHHPQHP
560 570 580 590 600
QHSLPSPDPD ILSVSSCPAL YRNEEEEEAI YFTAEKQWEV PDTASECDSL
610 620 630 640 650
NSSSGRKPSP PSSLEMYQTL SSRKISRDEL SLEDSSRGES PVTADVSRGS
660 670 680 690 700
PECVGLTETK SMIFSPASRV YNGILEKSCS MHQLSSGIPV PHPRHTSCSS
710 720 730 740
AGNDSKPVQE ASNVSRVSSI PHDLCHNGEK SKKPSKIKSL FKKKSK
Length:746
Mass (Da):83,925
Last modified:October 17, 2006 - v2
Checksum:iEE48455117B4E0BD
GO

Sequence cautioni

The sequence BAD32461.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173183 mRNA. Translation: BAD32461.1. Different initiation.
AF328907 mRNA. Translation: AAK82339.1.
BC043455 mRNA. Translation: AAH43455.1.
CCDSiCCDS39089.2.
RefSeqiNP_001074572.2. NM_001081103.2.
UniGeneiMm.265985.

Genome annotation databases

EnsembliENSMUST00000117661; ENSMUSP00000113174; ENSMUSG00000039156.
GeneIDi116873.
KEGGimmu:116873.
UCSCiuc008xlq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK173183 mRNA. Translation: BAD32461.1. Different initiation.
AF328907 mRNA. Translation: AAK82339.1.
BC043455 mRNA. Translation: AAH43455.1.
CCDSiCCDS39089.2.
RefSeqiNP_001074572.2. NM_001081103.2.
UniGeneiMm.265985.

3D structure databases

ProteinModelPortaliP83093.
SMRiP83093. Positions 62-205, 251-448.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000113174.

PTM databases

iPTMnetiP83093.

Proteomic databases

EPDiP83093.
MaxQBiP83093.
PaxDbiP83093.
PRIDEiP83093.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000117661; ENSMUSP00000113174; ENSMUSG00000039156.
GeneIDi116873.
KEGGimmu:116873.
UCSCiuc008xlq.2. mouse.

Organism-specific databases

CTDi57620.
MGIiMGI:2151156. Stim2.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4403. Eukaryota.
ENOG410XRM6. LUCA.
GeneTreeiENSGT00390000000214.
HOGENOMiHOG000261647.
HOVERGENiHBG079219.
InParanoidiP83093.
KOiK18196.
OMAiGTMAKPA.
OrthoDBiEOG7XSTDR.
PhylomeDBiP83093.
TreeFamiTF313487.

Miscellaneous databases

ChiTaRSiStim2. mouse.
PROiP83093.
SOURCEiSearch...

Gene expression databases

BgeeiP83093.
CleanExiMM_STIM2.
ExpressionAtlasiP83093. baseline and differential.
GenevisibleiP83093. MM.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR001660. SAM.
IPR013761. SAM/pointed.
IPR032393. SOAR.
[Graphical view]
PfamiPF07647. SAM_2. 1 hit.
PF16533. SOAR. 1 hit.
[Graphical view]
SMARTiSM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
PROSITEiPS50105. SAM_DOMAIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Prediction of the coding sequences of mouse homologues of KIAA gene: IV. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H., Nagase T., Ohara O., Koga H.
    DNA Res. 11:205-218(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Pancreatic islet.
  2. "Identification and characterization of the STIM (stromal interaction molecule) gene family: coding for a novel class of transmembrane proteins."
    Williams R.T., Manji S.S.M., Parker N.J., Hancock M.S., van Stekelenburg L., Eid J.-P., Senior P.V., Kazenwadel J.S., Shandala T., Saint R., Smith P.J., Dziadek M.A.
    Biochem. J. 357:673-685(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 82-227.
    Strain: C57BL/6J.
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 374-746.
    Tissue: Mammary gland.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-523, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "The phagosomal proteome in interferon-gamma-activated macrophages."
    Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
    Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28; SER-640 AND SER-650, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen and Testis.

Entry informationi

Entry nameiSTIM2_MOUSE
AccessioniPrimary (citable) accession number: P83093
Secondary accession number(s): Q69ZI3, Q80VF4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: October 17, 2006
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.