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P83059

- BRK2_BOMOR

UniProt

P83059 - BRK2_BOMOR

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Protein

Kininogen-2

Gene
N/A
Organism
Bombina orientalis (Oriental fire-bellied toad)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

[Thr6]-bradykinin: inhibits ACE with a Ki of 1.6 µM, and targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth muscle preparation (ileum). In vivo, induces an early hyperalgesic effects in living rats after intraplantar injection By similarity.By similarity
Bradykinin inhibitor peptide DV-28: inhibits the bradykinin-induced in vitro relaxation of rat arterial smooth muscle and constriction of intestinal smooth muscle. May target bradykinin receptors (BDKRB).2 Publications

GO - Biological processi

  1. defense response Source: UniProtKB
  2. negative regulation of blood pressure Source: UniProtKB
  3. positive regulation of smooth muscle contraction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Amphibian defense peptide, Bradykinin receptor impairing toxin, G-protein coupled receptor impairing toxin, Toxin

Names & Taxonomyi

Protein namesi
Recommended name:
Kininogen-2
Alternative name(s):
BOK-2
Cleaved into the following 2 chains:
OrganismiBombina orientalis (Oriental fire-bellied toad)Curated
Taxonomic identifieri8346 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraBombinatoridaeBombina

Subcellular locationi

Secreted 2 PublicationsCurated

GO - Cellular componenti

  1. extracellular region Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 161138Kininogen-2PRO_0000003434Add
BLAST
Peptidei87 – 959[Thr6]-bradykininPRO_0000003435
Peptidei115 – 1239[Thr6]-bradykininPRO_0000003436
Peptidei133 – 16028Bradykinin inhibitor peptide DV-28PRO_0000401148Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi150 ↔ 1561 Publication
Modified residuei160 – 1601Valine amide1 Publication

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the skin glands.2 Publications

Family & Domainsi

Sequence similaritiesi

Belongs to the bradykinin-related peptide family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOVERGENiHBG031664.

Family and domain databases

InterProiIPR009608. Bradykinin.
[Graphical view]
PfamiPF06753. Bradykinin. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83059-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRLWFCLSFF VVLCLEHFPG TLADERNNRD YPIRTHLHGP HIPRNNRDYP
60 70 80 90 100
IRTHLHGHHI PRNVPESEEK TEQFLRDLSE ISRLQRRPPG FTPFRGKFHS
110 120 130 140 150
QSLRDLSEIS RLQRRPPGFT PFRGKFHSQS LRDMYEIKGF KSAHGRPRVC
160
PPGEQCPIWV G
Length:161
Mass (Da):19,042
Last modified:October 19, 2002 - v2
Checksum:i200A7235C3657AAC
GO

Mass spectrometryi

Molecular mass is 3198.51 Da from positions 133 - 160. Determined by MALDI. The measured mass is that of bradykinin inhibitor peptide DV-28.1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ347942 mRNA. Translation: CAC70660.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ347942 mRNA. Translation: CAC70660.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

HOVERGENi HBG031664.

Family and domain databases

InterProi IPR009608. Bradykinin.
[Graphical view ]
Pfami PF06753. Bradykinin. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)."
    Chen T., Orr D.F., Bjourson A.J., McClean S., O'Rourke M., Hirst D.G., Rao P., Shaw C.
    Peptides 23:1547-1555(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-95 AND 115-123, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Skin and Skin secretion.
  2. "Peptide DV-28 amide: An inhibitor of bradykinin-induced arterial smooth muscle relaxation encoded by Bombina orientalis skin kininogen-2."
    Wang L., Chen Y., Yang M., Zhou M., Chen T., Sui D.Y., Shaw C.
    Peptides 31:979-982(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 133-160, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, DISULFIDE BOND, AMIDATION AT VAL-160.
    Tissue: Skin secretion.

Entry informationi

Entry nameiBRK2_BOMOR
AccessioniPrimary (citable) accession number: P83059
Secondary accession number(s): Q90W15
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: October 19, 2002
Last modified: October 1, 2014
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3