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P83059

- BRK2_BOMOR

UniProt

P83059 - BRK2_BOMOR

Protein

Kininogen-2

Gene
N/A
Organism
Bombina orientalis (Oriental fire-bellied toad)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    [Thr6]-bradykinin: inhibits ACE with a Ki of 1.6 µM, and targets B2 bradykinin receptor (BDKRB2). Provokes contraction of smooth muscle preparation (ileum). In vivo, induces an early hyperalgesic effects in living rats after intraplantar injection By similarity.By similarity
    Bradykinin inhibitor peptide DV-28: inhibits the bradykinin-induced in vitro relaxation of rat arterial smooth muscle and constriction of intestinal smooth muscle. May target bradykinin receptors (BDKRB).2 Publications

    GO - Biological processi

    1. defense response Source: UniProtKB
    2. negative regulation of blood pressure Source: UniProtKB
    3. positive regulation of smooth muscle contraction Source: UniProtKB

    Keywords - Molecular functioni

    Amphibian defense peptide, Bradykinin receptor impairing toxin, G-protein coupled receptor impairing toxin, Toxin

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kininogen-2
    Alternative name(s):
    BOK-2
    Cleaved into the following 2 chains:
    OrganismiBombina orientalis (Oriental fire-bellied toad)Curated
    Taxonomic identifieri8346 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraBombinatoridaeBombina

    Subcellular locationi

    Secreted 2 PublicationsCurated

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 161138Kininogen-2PRO_0000003434Add
    BLAST
    Peptidei87 – 959[Thr6]-bradykininPRO_0000003435
    Peptidei115 – 1239[Thr6]-bradykininPRO_0000003436
    Peptidei133 – 16028Bradykinin inhibitor peptide DV-28PRO_0000401148Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi150 ↔ 1561 Publication
    Modified residuei160 – 1601Valine amide1 Publication

    Keywords - PTMi

    Amidation, Disulfide bond

    Expressioni

    Tissue specificityi

    Expressed by the skin glands.2 Publications

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the bradykinin-related peptide family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG031664.

    Family and domain databases

    InterProiIPR009608. Bradykinin.
    [Graphical view]
    PfamiPF06753. Bradykinin. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P83059-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLWFCLSFF VVLCLEHFPG TLADERNNRD YPIRTHLHGP HIPRNNRDYP    50
    IRTHLHGHHI PRNVPESEEK TEQFLRDLSE ISRLQRRPPG FTPFRGKFHS 100
    QSLRDLSEIS RLQRRPPGFT PFRGKFHSQS LRDMYEIKGF KSAHGRPRVC 150
    PPGEQCPIWV G 161
    Length:161
    Mass (Da):19,042
    Last modified:October 19, 2002 - v2
    Checksum:i200A7235C3657AAC
    GO

    Mass spectrometryi

    Molecular mass is 3198.51 Da from positions 133 - 160. Determined by MALDI. The measured mass is that of bradykinin inhibitor peptide DV-28.1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ347942 mRNA. Translation: CAC70660.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ347942 mRNA. Translation: CAC70660.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG031664.

    Family and domain databases

    InterProi IPR009608. Bradykinin.
    [Graphical view ]
    Pfami PF06753. Bradykinin. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bradykinins and their precursor cDNAs from the skin of the fire-bellied toad (Bombina orientalis)."
      Chen T., Orr D.F., Bjourson A.J., McClean S., O'Rourke M., Hirst D.G., Rao P., Shaw C.
      Peptides 23:1547-1555(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 87-95 AND 115-123, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Skin and Skin secretion.
    2. "Peptide DV-28 amide: An inhibitor of bradykinin-induced arterial smooth muscle relaxation encoded by Bombina orientalis skin kininogen-2."
      Wang L., Chen Y., Yang M., Zhou M., Chen T., Sui D.Y., Shaw C.
      Peptides 31:979-982(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 133-160, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, DISULFIDE BOND, AMIDATION AT VAL-160.
      Tissue: Skin secretion.

    Entry informationi

    Entry nameiBRK2_BOMOR
    AccessioniPrimary (citable) accession number: P83059
    Secondary accession number(s): Q90W15
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2002
    Last sequence update: October 19, 2002
    Last modified: October 1, 2014
    This is version 44 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3