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P83056

- BRK1_BOMVA

UniProt

P83056 - BRK1_BOMVA

Protein

Kininogen-1

Gene
N/A
Organism
Bombina variegata (Yellow-bellied toad)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    [Ala3,Thr6]bradykinin: produces in vitro relaxation of rat arterial smooth muscle and constriction of intestinal smooth muscle. Possesses insulin-releasing activity. May target bradykinin receptors (BDKRB).2 Publications

    GO - Molecular functioni

    1. hormone activity Source: UniProtKB

    GO - Biological processi

    1. defense response Source: UniProtKB
    2. negative regulation of smooth muscle contraction Source: UniProtKB
    3. positive regulation of smooth muscle contraction Source: UniProtKB
    4. regulation of insulin secretion Source: UniProtKB
    5. vasodilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Amphibian defense peptide, G-protein coupled receptor impairing toxin, Toxin, Vasoactive, Vasodilator

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kininogen-1
    Alternative name(s):
    BVK-1
    Cleaved into the following 2 chains:
    OrganismiBombina variegata (Yellow-bellied toad)
    Taxonomic identifieri8348 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraBombinatoridaeBombina

    Subcellular locationi

    Secreted 2 Publications

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2323Sequence AnalysisAdd
    BLAST
    Chaini24 – 9774Kininogen-1Sequence AnalysisPRO_0000003437Add
    BLAST
    Peptidei51 – 599[Ala3,Thr6]-bradykininPRO_0000003438
    Peptidei76 – 9722Kininogen-1-associated peptidePRO_0000003439Add
    BLAST

    Keywords - PTMi

    Cleavage on pair of basic residues

    Expressioni

    Tissue specificityi

    Expressed by the skin glands.2 Publications

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the bradykinin-related peptide family.Curated

    Keywords - Domaini

    Repeat, Signal

    Phylogenomic databases

    HOVERGENiHBG031664.

    Family and domain databases

    InterProiIPR009608. Bradykinin.
    [Graphical view]
    PfamiPF06753. Bradykinin. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P83056-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRLWFCLSFL IILCVEHFPG TLAVERNVPE SEEKTEQFLR DLFEISRLQR   50
    RPAGFTPFRG KFHSQSLRGL SETKRIYNAI WPCKHCNKCK PGLLCKK 97
    Length:97
    Mass (Da):11,427
    Last modified:October 19, 2002 - v2
    Checksum:i1F038BEFFF0C0908
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 973CKK → AN AA sequence (PubMed:15134346)Curated

    Mass spectrometryi

    Molecular mass is 1049.31±0.08 Da from positions 51 - 59. Determined by ESI. 1 Publication
    Molecular mass is 2300.0 Da from positions 76 - 97. Determined by ESI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ320269 mRNA. Translation: CAC44903.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ320269 mRNA. Translation: CAC44903.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG031664.

    Family and domain databases

    InterProi IPR009608. Bradykinin.
    [Graphical view ]
    Pfami PF06753. Bradykinin. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin."
      Chen T., Orr D.F., Bjourson A.J., McClean S., O'Rourke M., Hirst D.G., Rao P., Shaw C.
      Eur. J. Biochem. 269:4693-4700(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 51-59, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Tissue: Skin1 Publication and Skin secretion1 Publication.
    2. "Skin secretion of the toad Bombina variegata contains multiple insulin-releasing peptides including bombesin and entirely novel insulinotropic structures."
      Marenah L., Flatt P.R., Orr D.F., McClean S., Shaw C., Abdel-Wahab Y.H.
      Biol. Chem. 385:315-321(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 76-97, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY.
      Tissue: Skin secretion1 Publication.

    Entry informationi

    Entry nameiBRK1_BOMVA
    AccessioniPrimary (citable) accession number: P83056
    Secondary accession number(s): Q90W86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 19, 2002
    Last sequence update: October 19, 2002
    Last modified: October 1, 2014
    This is version 48 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3