P83006 (PAFA2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet-activating factor acetylhydrolase 2, cytoplasmic
EC=3.1.1.47

Alternative name(s):
Serine-dependent phospholipase A2
Short name=SD-PLA2

Gene names

Name:

Pafah2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Also acts as a PAF-acetylhydrolase in the absence of lipid acceptor molecules. Ref.2
Catalytic activity	1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Monomer. Ref.2
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the serine esterase family. UniProtKB Q99487

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of apoptotic process Inferred from mutant phenotype PubMed 15456758. Source: RGD
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from mutant phenotype PubMed 15456758. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Platelet-activating factor acetylhydrolase 2, cytoplasmic		PRO_0000090385
Sites
	Active site	234	1	Nucleophile By similarity
	Active site	257	1	Charge relay system By similarity
	Active site	312	1	Charge relay system By similarity
Experimental info
	Sequence conflict	84	1	R → L AA sequence Ref.2
	Sequence conflict	95	1	T → K AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P83006 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 313FAC9553416373	FASTA	390	43,492
	10 20 30 40 50 60 MGAGQSICFP PISGPHHIGC TDVMEGHSLE GSLFRLFYPC EASETCEQPL WIPRYEYCVG 70 80 90 100 110 120 LADYLQYNKR WVGLLFNVGI GSCRLPVSWN GPFKTKESGY PLIILSHGLG GFRVSYSAFC 130 140 150 160 170 180 MELASRGFVV AAIEHRDQSA AATYFCKQTS QESSPTESLE EEWIPFRRIK EGEKEFHVRN 190 200 210 220 230 240 PQVHQRAKEC VRVLQILQDA SAGKPVINVF PGGLDLMTLK GSIDMSRVAV MGHSFGGATA 250 260 270 280 290 300 ILALTQEAQF RCAIALDAWM FPLEHDFYPK ARGPVFFINV EKFQTVESVN LMKKICAQHE 310 320 330 340 350 360 QSRIVTVLGA VHRSQTDFAF VTGNMIGKLF SSGTRGTLDP YEGQEVMVRA MLAFLQKHLD 370 380 390 LKEDYDQWNS FIEGIGPSLI QGAPHYLSSL

References

[1]	"Kinetics of platelet-activating factor binding, internalization, degradation, and apoptotic signaling in neural precursors." Bonin F., Moffat T.C., Renaud N., Franks D.J., Bennett S.A.L. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Brain.
[2]	"Purification and characterization from rat kidney membranes of a novel platelet-activating factor (PAF)-dependent transacetylase that catalyzes the hydrolysis of PAF, formation of PAF analogs, and C2-ceramide." Karasawa K., Qiu X., Lee T.-C. J. Biol. Chem. 274:8655-8661(1999) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 84-96; 169-179 AND 329-357. Strain: Sprague-Dawley. Tissue: Kidney.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY225592 mRNA. Translation: AAO62314.1.
IPI	IPI00331931.
RefSeq	NP_808793.1. NM_177932.2.
UniGene	Rn.92487.
3D structure databases
ProteinModelPortal	P83006.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000034439.
Proteomic databases
PaxDb	P83006.
PRIDE	P83006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000037097; ENSRNOP00000034439; ENSRNOG00000022288.
GeneID	313611.
KEGG	rno:313611.
UCSC	RGD:631399. rat.
Organism-specific databases
CTD	5051.
RGD	631399. Pafah2.
Phylogenomic databases
eggNOG	COG4188.
GeneTree	ENSGT00390000005233.
HOGENOM	HOG000008053.
HOVERGEN	HBG001322.
InParanoid	P83006.
KO	K01062.
OMA	CMELASR.
OrthoDB	EOG45DWPG.
Enzyme and pathway databases
BRENDA	3.1.1.47. 5301.
Gene expression databases
Genevestigator	P83006.
Family and domain databases
InterPro	IPR016715. Ac_Ohase_PAF. IPR005065. PAF_acetylhydro. [Graphical view]
PANTHER	PTHR10272. PTHR10272. 1 hit.
Pfam	PF03403. PAF-AH_p_II. 1 hit. [Graphical view]
PIRSF	PIRSF018169. PAF_acetylhydrolase. 1 hit.
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	666555.

Entry information

Entry name

PAFA2_RAT

Accession

Primary (citable) accession number: P83006
Secondary accession number(s): Q80Z89

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 31, 2004
Last sequence update:	August 31, 2004
Last modified:	April 3, 2013
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program