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P83006

- PAFA2_RAT

UniProt

P83006 - PAFA2_RAT

Protein

Platelet-activating factor acetylhydrolase 2, cytoplasmic

Gene

Pafah2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 79 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Also acts as a PAF-acetylhydrolase in the absence of lipid acceptor molecules.1 Publication

    Catalytic activityi

    1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei234 – 2341NucleophileBy similarity
    Active sitei257 – 2571Charge relay systemPROSITE-ProRule annotation
    Active sitei312 – 3121Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: RGD

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW
    2. negative regulation of apoptotic process Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Enzyme and pathway databases

    BRENDAi3.1.1.47. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-activating factor acetylhydrolase 2, cytoplasmic (EC:3.1.1.47)
    Alternative name(s):
    Serine-dependent phospholipase A2
    Short name:
    SD-PLA2
    Gene namesi
    Name:Pafah2
    OrganismiRattus norvegicus (Rat)Curated
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 5

    Organism-specific databases

    RGDi631399. Pafah2.

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 390390Platelet-activating factor acetylhydrolase 2, cytoplasmicPRO_0000090385Add
    BLAST

    Proteomic databases

    PaxDbiP83006.
    PRIDEiP83006.

    Expressioni

    Gene expression databases

    GenevestigatoriP83006.

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000034439.

    Structurei

    3D structure databases

    ProteinModelPortaliP83006.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serine esterase family.Curated

    Phylogenomic databases

    eggNOGiCOG4188.
    GeneTreeiENSGT00390000005233.
    HOGENOMiHOG000008053.
    HOVERGENiHBG001322.
    InParanoidiP83006.
    KOiK01062.
    OMAiQFRCAVA.
    OrthoDBiEOG7MSMQ5.
    PhylomeDBiP83006.
    TreeFamiTF313831.

    Family and domain databases

    Gene3Di3.40.50.1820. 2 hits.
    InterProiIPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view]
    PANTHERiPTHR10272. PTHR10272. 1 hit.
    PfamiPF03403. PAF-AH_p_II. 1 hit.
    [Graphical view]
    PIRSFiPIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMiSSF53474. SSF53474. 2 hits.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P83006-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGAGQSICFP PISGPHHIGC TDVMEGHSLE GSLFRLFYPC EASETCEQPL    50
    WIPRYEYCVG LADYLQYNKR WVGLLFNVGI GSCRLPVSWN GPFKTKESGY 100
    PLIILSHGLG GFRVSYSAFC MELASRGFVV AAIEHRDQSA AATYFCKQTS 150
    QESSPTESLE EEWIPFRRIK EGEKEFHVRN PQVHQRAKEC VRVLQILQDA 200
    SAGKPVINVF PGGLDLMTLK GSIDMSRVAV MGHSFGGATA ILALTQEAQF 250
    RCAIALDAWM FPLEHDFYPK ARGPVFFINV EKFQTVESVN LMKKICAQHE 300
    QSRIVTVLGA VHRSQTDFAF VTGNMIGKLF SSGTRGTLDP YEGQEVMVRA 350
    MLAFLQKHLD LKEDYDQWNS FIEGIGPSLI QGAPHYLSSL 390
    Length:390
    Mass (Da):43,492
    Last modified:August 31, 2004 - v2
    Checksum:i313FAC9553416373
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti84 – 841R → L AA sequence (PubMed:10085103)Curated
    Sequence conflicti95 – 951T → K AA sequence (PubMed:10085103)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY225592 mRNA. Translation: AAO62314.1.
    RefSeqiNP_808793.1. NM_177932.2.
    XP_006239188.1. XM_006239126.1.
    XP_006239189.1. XM_006239127.1.
    XP_006239190.1. XM_006239128.1.
    UniGeneiRn.92487.

    Genome annotation databases

    EnsembliENSRNOT00000037097; ENSRNOP00000034439; ENSRNOG00000022288.
    GeneIDi313611.
    KEGGirno:313611.
    UCSCiRGD:631399. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY225592 mRNA. Translation: AAO62314.1 .
    RefSeqi NP_808793.1. NM_177932.2.
    XP_006239188.1. XM_006239126.1.
    XP_006239189.1. XM_006239127.1.
    XP_006239190.1. XM_006239128.1.
    UniGenei Rn.92487.

    3D structure databases

    ProteinModelPortali P83006.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000034439.

    Proteomic databases

    PaxDbi P83006.
    PRIDEi P83006.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000037097 ; ENSRNOP00000034439 ; ENSRNOG00000022288 .
    GeneIDi 313611.
    KEGGi rno:313611.
    UCSCi RGD:631399. rat.

    Organism-specific databases

    CTDi 5051.
    RGDi 631399. Pafah2.

    Phylogenomic databases

    eggNOGi COG4188.
    GeneTreei ENSGT00390000005233.
    HOGENOMi HOG000008053.
    HOVERGENi HBG001322.
    InParanoidi P83006.
    KOi K01062.
    OMAi QFRCAVA.
    OrthoDBi EOG7MSMQ5.
    PhylomeDBi P83006.
    TreeFami TF313831.

    Enzyme and pathway databases

    BRENDAi 3.1.1.47. 5301.

    Miscellaneous databases

    NextBioi 666555.
    PROi P83006.

    Gene expression databases

    Genevestigatori P83006.

    Family and domain databases

    Gene3Di 3.40.50.1820. 2 hits.
    InterProi IPR029058. AB_hydrolase.
    IPR005065. PAF_acetylhydro.
    IPR016715. PAF_acetylhydro_eukaryote.
    [Graphical view ]
    PANTHERi PTHR10272. PTHR10272. 1 hit.
    Pfami PF03403. PAF-AH_p_II. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF018169. PAF_acetylhydrolase. 1 hit.
    SUPFAMi SSF53474. SSF53474. 2 hits.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Kinetics of platelet-activating factor binding, internalization, degradation, and apoptotic signaling in neural precursors."
      Bonin F., Moffat T.C., Renaud N., Franks D.J., Bennett S.A.L.
      Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Wistar.
      Tissue: Brain.
    2. "Purification and characterization from rat kidney membranes of a novel platelet-activating factor (PAF)-dependent transacetylase that catalyzes the hydrolysis of PAF, formation of PAF analogs, and C2-ceramide."
      Karasawa K., Qiu X., Lee T.-C.
      J. Biol. Chem. 274:8655-8661(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 84-96; 169-179 AND 329-357.
      Strain: Sprague-Dawley.
      Tissue: Kidney.

    Entry informationi

    Entry nameiPAFA2_RAT
    AccessioniPrimary (citable) accession number: P83006
    Secondary accession number(s): Q80Z89
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 79 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3