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Protein

Platelet-activating factor acetylhydrolase 2, cytoplasmic

Gene

Pafah2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme. Catalyzes transacetylation of the acetyl group from platelet-activating factor (PAF) to lysoplasmalogen and to sphingosine, producing plasmalogen analogs of PAF and N-acetylsphingosine (C2-ceramide) respectively. Acts as a PAF-acetylhydrolase in the absence of lipid acceptor molecules.1 Publication

Catalytic activityi

1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H2O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei234NucleophileBy similarity1
Active sitei257Charge relay systemPROSITE-ProRule annotation1
Active sitei312Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • 1-alkyl-2-acetylglycerophosphocholine esterase activity Source: RGD

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • negative regulation of apoptotic process Source: RGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism

Enzyme and pathway databases

BRENDAi3.1.1.47 5301
ReactomeiR-RNO-418346 Platelet homeostasis

Protein family/group databases

ESTHERiratno-paf2 PAF-Acetylhydrolase

Chemistry databases

SwissLipidsiSLP:000000697

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-activating factor acetylhydrolase 2, cytoplasmic1 Publication (EC:3.1.1.471 Publication)
Alternative name(s):
Serine-dependent phospholipase A2By similarity
Short name:
SD-PLA2Curated
Gene namesi
Name:Pafah2
OrganismiRattus norvegicus (Rat)Curated
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 5

Organism-specific databases

RGDi631399 Pafah2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Membrane, Microsome, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved
ChainiPRO_00000903852 – 390Platelet-activating factor acetylhydrolase 2, cytoplasmicAdd BLAST389

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycineBy similarity1

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP83006
PRIDEiP83006

PTM databases

iPTMnetiP83006
PhosphoSitePlusiP83006

Expressioni

Tissue specificityi

Expressed in kidney.1 Publication

Gene expression databases

BgeeiENSRNOG00000022288
ExpressionAtlasiP83006 baseline and differential

Interactioni

Subunit structurei

Monomer.1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000034439

Structurei

3D structure databases

ProteinModelPortaliP83006
SMRiP83006
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the serine esterase family.Curated

Phylogenomic databases

eggNOGiKOG3847 Eukaryota
ENOG4111FH0 LUCA
GeneTreeiENSGT00390000005233
HOGENOMiHOG000008053
HOVERGENiHBG001322
InParanoidiP83006
KOiK01062
OMAiCMELASR
OrthoDBiEOG091G08C2
PhylomeDBiP83006
TreeFamiTF313831

Family and domain databases

Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR005065 PAF_acetylhydro-like
IPR016715 PAF_acetylhydro_eukaryote
PANTHERiPTHR10272 PTHR10272, 1 hit
PfamiView protein in Pfam
PF03403 PAF-AH_p_II, 1 hit
PIRSFiPIRSF018169 PAF_acetylhydrolase, 1 hit
SUPFAMiSSF53474 SSF53474, 2 hits
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P83006-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGAGQSICFP PISGPHHIGC TDVMEGHSLE GSLFRLFYPC EASETCEQPL
60 70 80 90 100
WIPRYEYCVG LADYLQYNKR WVGLLFNVGI GSCRLPVSWN GPFKTKESGY
110 120 130 140 150
PLIILSHGLG GFRVSYSAFC MELASRGFVV AAIEHRDQSA AATYFCKQTS
160 170 180 190 200
QESSPTESLE EEWIPFRRIK EGEKEFHVRN PQVHQRAKEC VRVLQILQDA
210 220 230 240 250
SAGKPVINVF PGGLDLMTLK GSIDMSRVAV MGHSFGGATA ILALTQEAQF
260 270 280 290 300
RCAIALDAWM FPLEHDFYPK ARGPVFFINV EKFQTVESVN LMKKICAQHE
310 320 330 340 350
QSRIVTVLGA VHRSQTDFAF VTGNMIGKLF SSGTRGTLDP YEGQEVMVRA
360 370 380 390
MLAFLQKHLD LKEDYDQWNS FIEGIGPSLI QGAPHYLSSL
Length:390
Mass (Da):43,492
Last modified:August 31, 2004 - v2
Checksum:i313FAC9553416373
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti84R → L AA sequence (PubMed:10085103).Curated1
Sequence conflicti95T → K AA sequence (PubMed:10085103).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY225592 mRNA Translation: AAO62314.1
RefSeqiNP_808793.1, NM_177932.2
XP_006239189.1, XM_006239127.3
XP_017448893.1, XM_017593404.1
UniGeneiRn.92487

Genome annotation databases

EnsembliENSRNOT00000037097; ENSRNOP00000034439; ENSRNOG00000022288
GeneIDi313611
KEGGirno:313611
UCSCiRGD:631399 rat

Similar proteinsi

Entry informationi

Entry nameiPAFA2_RAT
AccessioniPrimary (citable) accession number: P83006
Secondary accession number(s): Q80Z89
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: August 31, 2004
Last modified: May 23, 2018
This is version 99 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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