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P82998 (GSTE_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione S-transferase

EC=2.5.1.18
Gene names
Ordered Locus Names:Pput_0205
OrganismPseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]
Taxonomic identifier351746 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length220 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Ref.2

Catalytic activity

RX + glutathione = HX + R-S-glutathione. Ref.2

Subunit structure

Monomer and homodimer. Ref.2

Subcellular location

Cytoplasm Ref.2.

Sequence similarities

Belongs to the GST superfamily. UniProtKB P45875

Contains 1 GST C-terminal domain.

Contains 1 GST N-terminal domain.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionTransferase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processmetabolic process

Non-traceable author statement Ref.2. Source: GOC

   Cellular_componentcytoplasm

Non-traceable author statement Ref.2. Source: UniProtKB

   Molecular_functionglutathione transferase activity

Non-traceable author statement Ref.2. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 220220Glutathione S-transferase
PRO_0000185982

Regions

Domain1 – 7777GST N-terminal
Domain82 – 211130GST C-terminal
Region61 – 622Glutathione binding By similarity

Sites

Binding site121Glutathione; via amide nitrogen By similarity
Binding site491Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P82998 [UniParc].

Last modified January 15, 2008. Version 2.
Checksum: 8559D8501EDB8A6E

FASTA22024,476
        10         20         30         40         50         60 
MLKLHGFSVS NYYNMVKLAL LEKGLPFEEV TFYGGQAPQA LEVSPRGKVP VLETEHGFLS 

        70         80         90        100        110        120 
ETSVILDYIE QTQSGKALLP ADPFEQAKVR ELLKEIELYI ELPARTCYAE SFFGMSVEPL 

       130        140        150        160        170        180 
IKEKARADLL AGFATLKRNG RFAPYVAGEQ LTLADLMFCF SVDLANAVGK KVLSIDFLAD 

       190        200        210        220 
FPQAKALLQL MGENPHMARI MADKEASMPA FMEMIRSGKR 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of Pseudomonas putida F1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. expand/collapse author list , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P.
Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: F1 / ATCC 700007.
[2]"Occurrence and properties of glutathione S-transferases in phenol-degrading Pseudomonas strains."
Santos P.M., Mignogna G., Heipieper H.J., Zennaro E.
Res. Microbiol. 153:89-98(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 1-21, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000712 Genomic DNA. Translation: ABQ76380.1.
RefSeqYP_001265564.1. NC_009512.1.

3D structure databases

ProteinModelPortalP82998.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING351746.Pput_0205.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABQ76380; ABQ76380; Pput_0205.
GeneID5192832.
KEGGppf:Pput_0205.
PATRIC19916363. VBIPsePut56420_0207.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0625.
HOGENOMHOG000125743.
KOK00799.
OMAYNMVKLA.
OrthoDBEOG6TN453.
ProtClustDBCLSK908438.

Enzyme and pathway databases

BioCycPPUT351746:GI26-209-MONOMER.

Family and domain databases

Gene3D1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamPF00043. GST_C. 1 hit.
PF13417. GST_N_3. 1 hit.
[Graphical view]
SUPFAMSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSTE_PSEP1
AccessionPrimary (citable) accession number: P82998
Secondary accession number(s): A5VWX0
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families