P82998 (GSTE_PSEP1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutathione S-transferase
EC=2.5.1.18

Gene names

Ordered Locus Names:

Pput_0205

Organism

Pseudomonas putida (strain F1 / ATCC 700007) [Complete proteome] [HAMAP]

Taxonomic identifier

351746 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	220 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Ref.2
Catalytic activity	RX + glutathione = HX + R-S-glutathione. Ref.2
Subunit structure	Monomer and homodimer. Ref.2
Subcellular location	Cytoplasm Ref.2.
Sequence similarities	Belongs to the GST superfamily. UniProtKB P45875 Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular_component	cytoplasm Non-traceable author statement Ref.2. Source: UniProtKB
Molecular_function	glutathione transferase activity Non-traceable author statement Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 220	220	Glutathione S-transferase		PRO_0000185982
Regions
	Domain	1 – 77	77	GST N-terminal
	Domain	82 – 211	130	GST C-terminal
	Region	61 – 62	2	Glutathione binding By similarity
Sites
	Binding site	12	1	Glutathione; via amide nitrogen By similarity
	Binding site	49	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P82998 [UniParc]. Last modified January 15, 2008. Version 2. Checksum: 8559D8501EDB8A6E	FASTA	220	24,476
	10 20 30 40 50 60 MLKLHGFSVS NYYNMVKLAL LEKGLPFEEV TFYGGQAPQA LEVSPRGKVP VLETEHGFLS 70 80 90 100 110 120 ETSVILDYIE QTQSGKALLP ADPFEQAKVR ELLKEIELYI ELPARTCYAE SFFGMSVEPL 130 140 150 160 170 180 IKEKARADLL AGFATLKRNG RFAPYVAGEQ LTLADLMFCF SVDLANAVGK KVLSIDFLAD 190 200 210 220 FPQAKALLQL MGENPHMARI MADKEASMPA FMEMIRSGKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete sequence of Pseudomonas putida F1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M. , Hauser L., Kyrpides N., Lykidis A., Parales R., Richardson P. Submitted (MAY-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: F1 / ATCC 700007.
[2]	"Occurrence and properties of glutathione S-transferases in phenol-degrading Pseudomonas strains." Santos P.M., Mignogna G., Heipieper H.J., Zennaro E. Res. Microbiol. 153:89-98(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-21, FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000712 Genomic DNA. Translation: ABQ76380.1.
RefSeq	YP_001265564.1. NC_009512.1.
3D structure databases
ProteinModelPortal	P82998.
ModBase	Search...
Protein-protein interaction databases
STRING	351746.Pput_0205.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABQ76380; ABQ76380; Pput_0205.
GeneID	5192832.
KEGG	ppf:Pput_0205.
PATRIC	19916363. VBIPsePut56420_0207.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0625.
HOGENOM	HOG000125743.
KO	K00799.
OMA	ELYIELP.
ProtClustDB	CLSK908438.
Enzyme and pathway databases
BioCyc	PPUT351746:GI26-303-MONOMER.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTE_PSEP1

Accession

Primary (citable) accession number: P82998
Secondary accession number(s): A5VWX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program