P82977 (ICIW_WHEAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Subtilisin-chymotrypsin inhibitor WSCI
Organism	Triticum aestivum (Wheat)
Taxonomic identifier	4565 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Liliopsida › commelinids › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Triticum

Protein attributes

Sequence length	84 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inhibits B.lichenoformis subtilisin, B.subtilis subtilisin, bovine pancreatic alpha-chymotrypsin and porcine alpha-chymotrypsin with Ki of 3.92 nM, 5.70 nM, 7.24 nM and 9.35 nM respectively. B.lichenoformis subtilisin is inhibited with a molar ratio of 1:0.87. Also inhibits chymotrypsin-like activities from the digestive tracts of the insect larvae T.molitor, P.interpunctella and H.armigera. Does not inhibit bovine pancreatic trypsin, porcine pancreatic elastase, or human leukocyte elastase. Ref.2 Ref.3
Subunit structure	Monomer. Ref.2
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the protease inhibitor I13 (potato type I serine protease inhibitor) family. [View classification]
Biophysicochemical properties	Temperature dependence: Optimum temperature is 25 degrees Celsius when incubation is carried out at pH 7.0-9.0. Ref.2
Mass spectrometry	Molecular mass is 8126.3±0.5 Da from positions 13 - 84. Determined by ESI. Ref.2

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor Serine protease inhibitor
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	response to wounding Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 12	12	Ref.2
	Chain	13 – 84	72	Subtilisin-chymotrypsin inhibitor WSCI		PRO_0000217651
Sites
	Site	60 – 61	2	Reactive bond
Experimental info
	Sequence conflict	51	1	V → I in AAY45744. Ref.1
	Sequence conflict	68	1	R → C in AAY45744. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P82977 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: E59B2374D25375C8	FASTA	84	9,326
	10 20 30 40 50 60 MSSVVKKPLG GNTDTGDHHN QKTEWPELVG KSVEEAKKVI LQDKSEAQIV VLPVGTIVTM 70 80 EYRIDRVRLF VDSLDKIAQV PRVG

References

[1]	"Bacterial expression of the WSCI gene and characterization of the recombinant product." Grassau B., Frenzel K., Baur X., Yu F. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Primary structure and reactive site of a novel wheat proteinase inhibitor of subtilisin and chymotrypsin." Poerio E., Di Gennaro S., Di Maro A., Farisei F., Ferranti P., Parente A. Biol. Chem. 384:295-304(2003) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 13-84, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, MASS SPECTROMETRY, REACTIVE SITE. Strain: cv. San Pastore. Tissue: Endosperm.
[3]	"cDNA cloning and heterologous expression of a wheat proteinase inhibitor of subtilisin and chymotrypsin (WSCI) that interferes with digestive enzymes of insect pests." Di Gennaro S., Ficca A., Panichi D., Poerio E. Biol. Chem. 386:383-389(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 13-84, FUNCTION. Strain: cv. San Pastore. Tissue: Immature kernel.

Cross-references

Sequence databases
EMBL GenBank DDBJ	DQ025758 mRNA. Translation: AAY45744.1. AJ422055 mRNA. Translation: CAD19324.1.
UniGene	Ta.1297. Ta.56587.
3D structure databases
ProteinModelPortal	P82977.
SMR	P82977. Positions 20-84.
ModBase	Search...
Protein family/group databases
Allergome	10784. Tri a 39.0101. 4075. Tri a 39.
MEROPS	I13.012.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	P82977.
Family and domain databases
InterPro	IPR000864. Prot_inh_pot1. [Graphical view]
Pfam	PF00280. potato_inhibit. 1 hit. [Graphical view]
PRINTS	PR00292. POTATOINHBTR.
ProDom	PD002604. Prot_inh_pot1. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54654. Prot_inh_pot1. 1 hit.
PROSITE	PS00285. POTATO_INHIBITOR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ICIW_WHEAT

Accession

Primary (citable) accession number: P82977
Secondary accession number(s): Q4TZQ0, Q546I0

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	January 9, 2007
Last modified:	May 1, 2013
This is version 55 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program