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Protein

Subtilisin-chymotrypsin inhibitor WSCI

Gene
N/A
Organism
Triticum aestivum (Wheat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits B.lichenoformis subtilisin, B.subtilis subtilisin, bovine pancreatic alpha-chymotrypsin and porcine alpha-chymotrypsin with Ki of 3.92 nM, 5.70 nM, 7.24 nM and 9.35 nM respectively. B.lichenoformis subtilisin is inhibited with a molar ratio of 1:0.87. Also inhibits chymotrypsin-like activities from the digestive tracts of the insect larvae T.molitor, P.interpunctella and H.armigera. Does not inhibit bovine pancreatic trypsin, porcine pancreatic elastase, or human leukocyte elastase.2 Publications

Temperature dependencei

Optimum temperature is 25 degrees Celsius when incubation is carried out at pH 7.0-9.0.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei60 – 612Reactive bond

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI13.012.

Names & Taxonomyi

Protein namesi
Recommended name:
Subtilisin-chymotrypsin inhibitor WSCI
OrganismiTriticum aestivum (Wheat)
Taxonomic identifieri4565 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeTriticinaeTriticum
Proteomesi
  • UP000019116 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Protein family/group databases

Allergomei10784. Tri a 39.0101.
4075. Tri a 39.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 12121 PublicationAdd
BLAST
Chaini13 – 8472Subtilisin-chymotrypsin inhibitor WSCIPRO_0000217651Add
BLAST

Interactioni

Subunit structurei

Monomer.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP82977.
SMRiP82977. Positions 20-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

InterProiIPR000864. Prot_inh_pot1.
[Graphical view]
PfamiPF00280. potato_inhibit. 1 hit.
[Graphical view]
PRINTSiPR00292. POTATOINHBTR.
ProDomiPD002604. Prot_inh_pot1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54654. SSF54654. 1 hit.
PROSITEiPS00285. POTATO_INHIBITOR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82977-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSVVKKPLG GNTDTGDHHN QKTEWPELVG KSVEEAKKVI LQDKSEAQIV
60 70 80
VLPVGTIVTM EYRIDRVRLF VDSLDKIAQV PRVG
Length:84
Mass (Da):9,326
Last modified:January 9, 2007 - v2
Checksum:iE59B2374D25375C8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511V → I in AAY45744 (Ref. 1) Curated
Sequence conflicti68 – 681R → C in AAY45744 (Ref. 1) Curated

Mass spectrometryi

Molecular mass is 8126.3±0.5 Da from positions 13 - 84. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ025758 mRNA. Translation: AAY45744.1.
AJ422055 mRNA. Translation: CAD19324.1.
UniGeneiTa.56587.
Ta.97092.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ025758 mRNA. Translation: AAY45744.1.
AJ422055 mRNA. Translation: CAD19324.1.
UniGeneiTa.56587.
Ta.97092.

3D structure databases

ProteinModelPortaliP82977.
SMRiP82977. Positions 20-84.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei10784. Tri a 39.0101.
4075. Tri a 39.
MEROPSiI13.012.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR000864. Prot_inh_pot1.
[Graphical view]
PfamiPF00280. potato_inhibit. 1 hit.
[Graphical view]
PRINTSiPR00292. POTATOINHBTR.
ProDomiPD002604. Prot_inh_pot1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SUPFAMiSSF54654. SSF54654. 1 hit.
PROSITEiPS00285. POTATO_INHIBITOR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiICIW_WHEAT
AccessioniPrimary (citable) accession number: P82977
Secondary accession number(s): Q4TZQ0, Q546I0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 9, 2007
Last modified: February 17, 2016
This is version 67 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.