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P82973

- AMPD_ENTCL

UniProt

P82973 - AMPD_ENTCL

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Protein
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Gene
ampD
Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.

Catalytic activityi

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

Cofactori

Zinc; required for amidase activity By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Zinc; catalytic By similarity
Active sitei116 – 1161Proton acceptor By similarity
Metal bindingi154 – 1541Zinc; catalytic By similarity
Sitei162 – 1621Transition state stabilizer By similarity
Metal bindingi164 – 1641Zinc; catalytic By similarity

GO - Molecular functioni

  1. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. peptidoglycan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
Alternative name(s):
N-acetylmuramoyl-L-alanine amidase
Gene namesi
Name:ampD
OrganismiEnterobacter cloacae
Taxonomic identifieri550 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1871871,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
PRO_0000164412Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP82973.
SMRiP82973. Positions 1-187.

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.80.10. 1 hit.
InterProiIPR002502. Amidase_domain.
[Graphical view]
PfamiPF01510. Amidase_2. 1 hit.
[Graphical view]
SMARTiSM00644. Ami_2. 1 hit.
[Graphical view]
SUPFAMiSSF55846. SSF55846. 1 hit.

Sequencei

Sequence statusi: Complete.

P82973-1 [UniParc]FASTAAdd to Basket

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MLLENGWLVD ARHVPSPHHD CRPEDEKPTL LVVHNISLPP GEFGGPWIDA    50
LFTGTIDPDA HPFFAEIAHL ALSADCLIRR DGEVVQYVPF DKRAWHAGVS 100
MYQGRERCND FSIGIELEGT DTTPYTDAQY EKLVAVTQTL IGRYPAIADN 150
ITGHSDIAPE RKTDPGPAFD WSRFHAMLTT SSDKEIT 187
Length:187
Mass (Da):20,839
Last modified:November 2, 2001 - v1
Checksum:iF1D23B55B84E71C8
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14003 Genomic DNA. Translation: CAA78391.1.
PIRiA48901.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z14003 Genomic DNA. Translation: CAA78391.1 .
PIRi A48901.

3D structure databases

ProteinModelPortali P82973.
SMRi P82973. Positions 1-187.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.40.80.10. 1 hit.
InterProi IPR002502. Amidase_domain.
[Graphical view ]
Pfami PF01510. Amidase_2. 1 hit.
[Graphical view ]
SMARTi SM00644. Ami_2. 1 hit.
[Graphical view ]
SUPFAMi SSF55846. SSF55846. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequences of wild-type and mutant ampD genes of Citrobacter freundii and Enterobacter cloacae."
    Kopp U., Wiedemann B., Lindquist S., Normark S.
    Antimicrob. Agents Chemother. 37:224-228(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 14.

Entry informationi

Entry nameiAMPD_ENTCL
AccessioniPrimary (citable) accession number: P82973
Secondary accession number(s): Q00831
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2001
Last sequence update: November 2, 2001
Last modified: July 9, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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