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Protein

Phospholipase A2

Gene
N/A
Organism
Bombus terrestris (Buff-tailed bumblebee) (Apis terrestris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Calcium; via carbonyl oxygenBy similarity
Metal bindingi10 – 101Calcium; via carbonyl oxygenBy similarity
Metal bindingi12 – 121Calcium; via carbonyl oxygenBy similarity
Active sitei34 – 341PROSITE-ProRule annotation
Metal bindingi35 – 351CalciumBy similarity
Active sitei64 – 641PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A2 (EC:3.1.1.4)
Short name:
PLA2
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
Allergen: Bom t 1
OrganismiBombus terrestris (Buff-tailed bumblebee) (Apis terrestris)
Taxonomic identifieri30195 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaHymenopteraApocritaAculeataApoideaApidaeBombusBombus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human.1 Publication

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei155. Bom t 1.
3159. Bom t 1.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 136136Phospholipase A2PRO_0000161724Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi9 ↔ 31By similarity
Glycosylationi16 – 161N-linked (GlcNAc...)
Disulfide bondi30 ↔ 70By similarity
Disulfide bondi37 ↔ 63By similarity
Disulfide bondi61 ↔ 95By similarity
Disulfide bondi105 ↔ 117By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Expressioni

Tissue specificityi

Expressed by the venom gland.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP82971.
SMRiP82971. Positions 1-136.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PfamiPF05826. Phospholip_A2_2. 1 hit.
[Graphical view]
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P82971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
IIFPGTLWCG NGNLANGTNQ LGSWKETDSC CRTHDMCPDL IEAHGSKHGL
60 70 80 90 100
TNAADYTRLS CECDEEFRRC LHNSGDTVSA GFVGRTYFTV LHTQCFRLDY
110 120 130
PIVKCKVKST ILHRSKCYDF ETFAPKKYQW FDVLQY
Length:136
Mass (Da):15,546
Last modified:June 20, 2001 - v1
Checksum:i07797BDDEC74F20E
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP82971.
SMRiP82971. Positions 1-136.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei155. Bom t 1.
3159. Bom t 1.0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PfamiPF05826. Phospholip_A2_2. 1 hit.
[Graphical view]
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Occupational allergy to bumblebees: allergens of Bombus terrestris."
    Hoffman D.R., El-Choufani S.E., Smith M.M., de Groot H.
    J. Allergy Clin. Immunol. 108:855-860(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ALLERGEN.
    Tissue: Venom.

Entry informationi

Entry nameiPA2_BOMTE
AccessioniPrimary (citable) accession number: P82971
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: June 20, 2001
Last modified: May 11, 2016
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.