P82965 (ADFA_TENMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Antidiuretic factor A Short name=ADF Short name=ADFa Alternative name(s): Antidiuretic hormone A Short name=ADHA
Organism	Tenebrio molitor (Yellow mealworm beetle)
Taxonomic identifier	7067 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Coleoptera › Polyphaga › Cucujiformia › Tenebrionoidea › Tenebrionidae › Tenebrioninae › Tenebrio

Protein attributes

Sequence length	14 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Strong inhibitor of fluid secretion by the Malpighian tubules. Uses cGMP as a second messenger and inhibits fluid production by decreasing cAMP concentration. Reduces isosmotic fluid secretion by inhibiting electroneutral and non-conductive transport pathways. Reverses the stimulatory effect of diuretic hormone I. Ref.1 Ref.2 Ref.3
Subcellular location	Secreted.
Sequence similarities	To T.molitor cuticular protein LPCP29 C-terminal region.
Mass spectrometry	Molecular mass is 1541.58 Da from positions 1 - 14. Determined by MALDI. Ref.1

Ontologies

Keywords
Cellular component	Secreted
Molecular function	Hormone Neuropeptide
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	neuropeptide signaling pathway Non-traceable author statement Ref.1. Source: UniProtKB
Cellular_component	extracellular region Non-traceable author statement Ref.1. Source: UniProtKB
Molecular_function	neuropeptide hormone activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Peptide	1 – 14	14	Antidiuretic factor A		PRO_0000044103

Sequences

	Sequence		Length	Mass (Da)
	P82965 [UniParc]. Last modified March 1, 2002. Version 1. Checksum: F49C91A3F16E43D1	FASTA	14	1,543
	10 VVNTPGHAVS YHVY

References

[1]	"Identification of a potent antidiuretic factor acting on beetle Malpighian tubules." Eigenheer R.A., Nicolson S.W., Schegg K.M., Hull J.J., Schooley D.A. Proc. Natl. Acad. Sci. U.S.A. 99:84-89(2002) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, TISSUE SPECIFICITY, MASS SPECTROMETRY. Tissue: Head.
[2]	"Antagonistic control of fluid secretion by the Malpighian tubules of Tenebrio molitor: effects of diuretic and antidiuretic peptides and their second messengers." Wiehart U.I.M., Nicolson S.W., Eigenheer R.A., Schooley D.A. J. Exp. Biol. 205:493-501(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"The mechanism of action of the antidiuretic peptide Tenmo ADFa in Malpighian tubules of Aedes aegypti." Massaro R.C., Lee L.W., Patel A.B., Wu D.S., Yu M.-J., Scott B.N., Schooley D.A., Schegg K.M., Beyenbach K.W. J. Exp. Biol. 207:2877-2888(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ADFA_TENMO

Accession

Primary (citable) accession number: P82965

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2002
Last sequence update:	March 1, 2002
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)