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P82891

- PPAC2_DROME

UniProt

P82891 - PPAC2_DROME

Protein

Low molecular weight phosphotyrosine protein phosphatase 2

Gene

primo-2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 106 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei14 – 141NucleophileBy similarity
    Active sitei20 – 201By similarity
    Active sitei130 – 1301Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. non-membrane spanning protein tyrosine phosphatase activity Source: InterPro
    3. protein tyrosine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. peptidyl-tyrosine dephosphorylation Source: GOC
    2. protein dephosphorylation Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight phosphotyrosine protein phosphatase 2 (EC:3.1.3.48)
    Alternative name(s):
    Low molecular weight cytosolic acid phosphatase 2 (EC:3.1.3.2)
    PTPase 2
    Gene namesi
    Name:primo-2
    ORF Names:CG31311
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0040076. primo-2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi14 – 141C → S: Complete loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 164164Low molecular weight phosphotyrosine protein phosphatase 2PRO_0000046563Add
    BLAST

    Proteomic databases

    PRIDEiP82891.

    Expressioni

    Tissue specificityi

    Cone cells and primary pigment cells in developing pupal retina.1 Publication

    Developmental stagei

    Embryo and adult.1 Publication

    Gene expression databases

    BgeeiP82891.

    Structurei

    3D structure databases

    ProteinModelPortaliP82891.
    SMRiP82891. Positions 9-158.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0394.
    GeneTreeiENSGT00500000044891.
    InParanoidiP82891.
    KOiK14394.
    OMAiGNEYPAN.
    OrthoDBiEOG7QZGCG.
    PhylomeDBiP82891.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR002115. Tyr_Pase_low_mol_wt_mml.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    PR00720. MAMMALPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P82891-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKRSQKSSV LMVCVGNLCR SPIAEAVMRD LVARAGLQGE WHVESAGIED    50
    WHSGHQPDER ALNVLARHNI EYNGKARVLA PEDFLEFDYI FAMDLSNLAA 100
    LRRMAPKGTT AKLLILGNFG LKPDERIIED PYYDIGEASF EEIYRQCSIA 150
    CRNFLKQARL KQIM 164
    Length:164
    Mass (Da):18,541
    Last modified:March 1, 2001 - v1
    Checksum:i320485DF557E4CCD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti31 – 311L → V(PubMed:10675607)Curated
    Sequence conflicti111 – 1111A → V(PubMed:10675607)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAN13590.2.
    AY089477 mRNA. Translation: AAL90215.1.
    RefSeqiNP_001027188.1. NM_001032017.2.
    UniGeneiDm.4988.

    Genome annotation databases

    EnsemblMetazoaiFBtr0091746; FBpp0100064; FBgn0040076.
    GeneIDi3772427.
    KEGGidme:Dmel_CG33747.
    UCSCiCG33747-RB. d. melanogaster.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAN13590.2 .
    AY089477 mRNA. Translation: AAL90215.1 .
    RefSeqi NP_001027188.1. NM_001032017.2.
    UniGenei Dm.4988.

    3D structure databases

    ProteinModelPortali P82891.
    SMRi P82891. Positions 9-158.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P82891.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0091746 ; FBpp0100064 ; FBgn0040076 .
    GeneIDi 3772427.
    KEGGi dme:Dmel_CG33747.
    UCSCi CG33747-RB. d. melanogaster.

    Organism-specific databases

    CTDi 3772427.
    FlyBasei FBgn0040076. primo-2.

    Phylogenomic databases

    eggNOGi COG0394.
    GeneTreei ENSGT00500000044891.
    InParanoidi P82891.
    KOi K14394.
    OMAi GNEYPAN.
    OrthoDBi EOG7QZGCG.
    PhylomeDBi P82891.

    Miscellaneous databases

    GenomeRNAii 3772427.
    NextBioi 853692.

    Gene expression databases

    Bgeei P82891.

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR002115. Tyr_Pase_low_mol_wt_mml.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    PR00720. MAMMALPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Drosophila primo locus encodes two low-molecular-weight tyrosine phosphatases."
      Miller D.T., Read R., Rusconi J., Cagan R.L.
      Gene 243:1-9(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-14, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Embryo and Pupae.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
      Tissue: Testis.

    Entry informationi

    Entry nameiPPAC2_DROME
    AccessioniPrimary (citable) accession number: P82891
    Secondary accession number(s): Q0KI75, Q9VFR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 106 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3