Reviewed, UniProtKB/Swiss-Prot P82891 (PPAC2_DROME)

Names and origin

Protein names

Recommended name:
    Low molecular weight phosphotyrosine protein phosphatase 2
    EC=3.1.3.48
Alternative name(s):
    Low molecular weight cytosolic acid phosphatase 2
    EC=3.1.3.2
    PTPase 2

Gene names

Name:	primo-2
ORF Names:	CG31311

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	164 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Cytoplasm.
Tissue specificity	Cone cells and primary pigment cells in developing pupal retina. Ref.1
Developmental stage	Embryo and adult. Ref.1
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Non-traceable author statement. Source: FlyBase
Cellular component	cytoplasm Non-traceable author statement. Source: FlyBase
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC non-membrane spanning protein tyrosine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 164	164	Low molecular weight phosphotyrosine protein phosphatase 2		PRO_0000046563
Sites
	Active site	14	1	Nucleophile By similarity
	Active site	20	1	By similarity
	Active site	130	1	Proton donor By similarity
Experimental info
	Mutagenesis	14	1	C → S: Complete loss of activity. Ref.1
	Sequence conflict	31	1	L → V Ref.1
	Sequence conflict	111	1	A → V Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P82891-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 320485DF557E4CCD	FASTA	164	18,541
	10 20 30 40 50 60 MGKRSQKSSV LMVCVGNLCR SPIAEAVMRD LVARAGLQGE WHVESAGIED WHSGHQPDER 70 80 90 100 110 120 ALNVLARHNI EYNGKARVLA PEDFLEFDYI FAMDLSNLAA LRRMAPKGTT AKLLILGNFG 130 140 150 160 LKPDERIIED PYYDIGEASF EEIYRQCSIA CRNFLKQARL KQIM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila primo locus encodes two low-molecular-weight tyrosine phosphatases." Miller D.T., Read R., Rusconi J., Cagan R.L. Gene 243:1-9(2000) [PubMed: 10675607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-14, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo and Pupae.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014297 Genomic DNA. Translation: AAN13590.2. AY089477 mRNA. Translation: AAL90215.1.
RefSeq	NP_001027188.1. NP_001027189.1.
UniGene	Dm.4988
3D structure databases
SMR	P82891. Positions 9-157.
ModBase	Search...
Proteomic databases
PRIDE	P82891.
Genome annotation databases
Ensembl	FBtr0091745; FBpp0100063; FBgn0040076; Drosophila melanogaster. [Genome view] FBtr0091746; FBpp0100064; FBgn0040076; Drosophila melanogaster. [Genome view]
GeneID	3772427.
KEGG	dme:Dmel_CG33747.
UCSC	CG33747-RB. d. melanogaster.
Organism-specific databases
CTD	3772427.
FlyBase	FBgn0040076. primo-2.
Phylogenomic databases
eggNOG	inNOG10293.
InParanoid	P82891.
OMA	ATSGWEH.
OrthoDB	EOG9V43FB.
PhylomeDB	P82891.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-011492-MONOMER. DMEL-XXX-02:DMEL-XXX-02-011494-MONOMER.
BRENDA	3.1.3.2. 48. 3.1.3.48. 48.
Gene expression databases
GermOnline	CG33747. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002115. Tyr_Pase_low_mol_wt_mml. IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE. PR00720. MAMMALPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	853692.

Entry information

Entry name

PPAC2_DROME

Accession

Primary (citable) accession number: P82891
Secondary accession number(s): Q0KI75, Q9VFR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	January 19, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project