Reviewed, UniProtKB/Swiss-Prot P82890 (PPAC1_DROME)

Names and origin

Protein names

Recommended name:
    Low molecular weight phosphotyrosine protein phosphatase 1
    EC=3.1.3.48
Alternative name(s):
    Low molecular weight cytosolic acid phosphatase 1
    EC=3.1.3.2
    PTPase 1

Gene names

Name:	primo-1
ORF Names:	CG31311

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	155 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Cytoplasm.
Tissue specificity	Cone cells and primary pigment cells in developing pupal retina. Ref.1
Developmental stage	Larvae, pupae and adults. Ref.1
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid dephosphorylation Non-traceable author statement. Source: FlyBase
Cellular component	cytoplasm Non-traceable author statement. Source: FlyBase
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC non-membrane spanning protein tyrosine phosphatase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform A (identifier: P82890-1)

Also known as: B;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform C (identifier: P82890-2)

The sequence of this isoform differs from the canonical sequence as follows:
     128-131: ERGA → VRYF
     132-155: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 155	155	Low molecular weight phosphotyrosine protein phosphatase 1		PRO_0000046562
Sites
	Active site	9	1	Nucleophile By similarity
	Active site	15	1	By similarity
	Active site	124	1	Proton donor By similarity
Natural variations
	Alternative sequence	128 – 131	4	ERGA → VRYF in isoform C.		VSP_007676
	Alternative sequence	132 – 155	24	Missing in isoform C.		VSP_007677

Sequences

	Sequence		Length	Mass (Da)
	Isoform A (B) [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 26C05D1EC3B1A07C	FASTA	155	17,556
	10 20 30 40 50 60 MVRKVLMICL GNICRSPIAE VVMVDTLEKA NVKDVEVDSA AIGGWHVGNR ADPRAISTLQ 70 80 90 100 110 120 KHGLKCTHIV RQIRKQDFSE FDYIFGMDED NMSELRRLAP KGSKAELLML GDFGLEKKNR 130 140 150 IIEDPYYERG AEGFETAYQQ CVVACAAFMK ERLQK
	Isoform C. Checksum: 19DBF93C31A19250	FASTA	131	14,975
	10 20 30 40 50 60 MVRKVLMICL GNICRSPIAE VVMVDTLEKA NVKDVEVDSA AIGGWHVGNR ADPRAISTLQ 70 80 90 100 110 120 KHGLKCTHIV RQIRKQDFSE FDYIFGMDED NMSELRRLAP KGSKAELLML GDFGLEKKNR 130 IIEDPYYVRY F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila primo locus encodes two low-molecular-weight tyrosine phosphatases." Miller D.T., Read R., Rusconi J., Cagan R.L. Gene 243:1-9(2000) [PubMed: 10675607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Tissue: Embryo and Pupae.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE014297 Genomic DNA. Translation: AAN13591.2. AE014297 Genomic DNA. Translation: AAO41564.1. AE014297 Genomic DNA. Translation: AAO41565.1.
RefSeq	NP_001027185.1. NP_001027186.1. NP_001027187.1.
UniGene	Dm.4988
3D structure databases
HSSP	HSSP built from PDB template 1DG9 based on UniProtKB P11064.
ModBase	Search...
Proteomic databases
PRIDE	P82890.
Genome annotation databases
Ensembl	FBtr0091747; FBpp0099893; FBgn0040077; Drosophila melanogaster. [Genome view]
GeneID	3772179.
KEGG	dme:Dmel_CG33748.
UCSC	CG33748-RA. d. melanogaster.
Organism-specific databases
CTD	3772179.
FlyBase	FBgn0040077. primo-1.
Phylogenomic databases
HOGENOM	P82890.
OMA	AIGSWHV.
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-011493-MON. DMEL-XXX-02:DMEL-XXX-02-011496-MON.
BRENDA	3.1.3.2. 48. 3.1.3.48. 48.
Gene expression databases
GermOnline	CG33748. Drosophila melanogaster.
Family and domain databases
InterPro	IPR002115. Tyr_Pase_low_mol_wt_mml. IPR000106. Tyr_phospatase/Ars_reductase. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
PRINTS	PR00719. LMWPTPASE. PR00720. MAMMALPTPASE.
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	852731.

Entry information

Entry name

PPAC1_DROME

Accession

Primary (citable) accession number: P82890
Secondary accession number(s): A4V2V3, Q9VFR9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	March 1, 2001
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project