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P82890

- PPAC1_DROME

UniProt

P82890 - PPAC1_DROME

Protein

Low molecular weight phosphotyrosine protein phosphatase 1

Gene

primo-1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Mar 2001)
      Previous versions | rss
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    Functioni

    Acts on tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.
    A phosphate monoester + H2O = an alcohol + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei9 – 91NucleophileBy similarity
    Active sitei15 – 151By similarity
    Active sitei124 – 1241Proton donorBy similarity

    GO - Molecular functioni

    1. acid phosphatase activity Source: UniProtKB-EC
    2. non-membrane spanning protein tyrosine phosphatase activity Source: InterPro
    3. protein tyrosine phosphatase activity Source: FlyBase

    GO - Biological processi

    1. peptidyl-tyrosine dephosphorylation Source: GOC
    2. protein dephosphorylation Source: FlyBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Low molecular weight phosphotyrosine protein phosphatase 1 (EC:3.1.3.48)
    Alternative name(s):
    Low molecular weight cytosolic acid phosphatase 1 (EC:3.1.3.2)
    PTPase 1
    Gene namesi
    Name:primo-1
    ORF Names:CG31311
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 3R

    Organism-specific databases

    FlyBaseiFBgn0040077. primo-1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: FlyBase

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 155155Low molecular weight phosphotyrosine protein phosphatase 1PRO_0000046562Add
    BLAST

    Proteomic databases

    PaxDbiP82890.
    PRIDEiP82890.

    Expressioni

    Tissue specificityi

    Cone cells and primary pigment cells in developing pupal retina.1 Publication

    Developmental stagei

    Larvae, pupae and adults.1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi7227.FBpp0099895.

    Structurei

    3D structure databases

    ProteinModelPortaliP82890.
    SMRiP82890. Positions 3-151.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0394.
    GeneTreeiENSGT00500000044891.
    InParanoidiP82890.
    KOiK14394.
    OMAiEIMSADL.
    OrthoDBiEOG7QZGCG.
    PhylomeDBiP82890.

    Family and domain databases

    InterProiIPR023485. Ptyr_pPase_SF.
    IPR002115. Tyr_Pase_low_mol_wt_mml.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view]
    PANTHERiPTHR11717. PTHR11717. 1 hit.
    PfamiPF01451. LMWPc. 1 hit.
    [Graphical view]
    PRINTSiPR00719. LMWPTPASE.
    PR00720. MAMMALPTPASE.
    SMARTiSM00226. LMWPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52788. SSF52788. 1 hit.

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform A (identifier: P82890-1) [UniParc]FASTAAdd to Basket

    Also known as: B

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVRKVLMICL GNICRSPIAE VVMVDTLEKA NVKDVEVDSA AIGGWHVGNR    50
    ADPRAISTLQ KHGLKCTHIV RQIRKQDFSE FDYIFGMDED NMSELRRLAP 100
    KGSKAELLML GDFGLEKKNR IIEDPYYERG AEGFETAYQQ CVVACAAFMK 150
    ERLQK 155
    Length:155
    Mass (Da):17,556
    Last modified:March 1, 2001 - v1
    Checksum:i26C05D1EC3B1A07C
    GO
    Isoform C (identifier: P82890-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         128-131: ERGA → VRYF
         132-155: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:131
    Mass (Da):14,975
    Checksum:i19DBF93C31A19250
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei128 – 1314ERGA → VRYF in isoform C. CuratedVSP_007676
    Alternative sequencei132 – 15524Missing in isoform C. CuratedVSP_007677Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAN13591.2.
    AE014297 Genomic DNA. Translation: AAO41564.1.
    AE014297 Genomic DNA. Translation: AAO41565.1.
    RefSeqiNP_001027186.1. NM_001032015.2. [P82890-1]
    NP_001027187.1. NM_001032016.2. [P82890-1]
    UniGeneiDm.4988.

    Genome annotation databases

    EnsemblMetazoaiFBtr0091747; FBpp0099893; FBgn0040077. [P82890-1]
    FBtr0091748; FBpp0099895; FBgn0040077. [P82890-1]
    GeneIDi3772179.
    KEGGidme:Dmel_CG33748.
    UCSCiCG33748-RA. d. melanogaster. [P82890-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE014297 Genomic DNA. Translation: AAN13591.2 .
    AE014297 Genomic DNA. Translation: AAO41564.1 .
    AE014297 Genomic DNA. Translation: AAO41565.1 .
    RefSeqi NP_001027186.1. NM_001032015.2. [P82890-1 ]
    NP_001027187.1. NM_001032016.2. [P82890-1 ]
    UniGenei Dm.4988.

    3D structure databases

    ProteinModelPortali P82890.
    SMRi P82890. Positions 3-151.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 7227.FBpp0099895.

    Proteomic databases

    PaxDbi P82890.
    PRIDEi P82890.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0091747 ; FBpp0099893 ; FBgn0040077 . [P82890-1 ]
    FBtr0091748 ; FBpp0099895 ; FBgn0040077 . [P82890-1 ]
    GeneIDi 3772179.
    KEGGi dme:Dmel_CG33748.
    UCSCi CG33748-RA. d. melanogaster. [P82890-1 ]

    Organism-specific databases

    CTDi 3772179.
    FlyBasei FBgn0040077. primo-1.

    Phylogenomic databases

    eggNOGi COG0394.
    GeneTreei ENSGT00500000044891.
    InParanoidi P82890.
    KOi K14394.
    OMAi EIMSADL.
    OrthoDBi EOG7QZGCG.
    PhylomeDBi P82890.

    Miscellaneous databases

    GenomeRNAii 3772179.
    NextBioi 852731.
    PROi P82890.

    Family and domain databases

    InterProi IPR023485. Ptyr_pPase_SF.
    IPR002115. Tyr_Pase_low_mol_wt_mml.
    IPR000106. Tyr_phospatase/Ars_reductase.
    IPR017867. Tyr_phospatase_low_mol_wt.
    [Graphical view ]
    PANTHERi PTHR11717. PTHR11717. 1 hit.
    Pfami PF01451. LMWPc. 1 hit.
    [Graphical view ]
    PRINTSi PR00719. LMWPTPASE.
    PR00720. MAMMALPTPASE.
    SMARTi SM00226. LMWPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52788. SSF52788. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The Drosophila primo locus encodes two low-molecular-weight tyrosine phosphatases."
      Miller D.T., Read R., Rusconi J., Cagan R.L.
      Gene 243:1-9(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Embryo and Pupae.
    2. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    3. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
      Strain: Berkeley.

    Entry informationi

    Entry nameiPPAC1_DROME
    AccessioniPrimary (citable) accession number: P82890
    Secondary accession number(s): A4V2V3, Q9VFR9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: March 1, 2001
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3