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Protein

Mitochondrial import receptor subunit TOM20-3

Gene

TOM20-3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore.1 Publication

GO - Molecular functioni

GO - Biological processi

  • protein import into mitochondrial outer membrane Source: InterPro
  • protein targeting to mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM20-3
Alternative name(s):
Translocase of outer membrane 20 kDa subunit 3
Gene namesi
Name:TOM20-3
Ordered Locus Names:At3g27080
ORF Names:MOJ10.15
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G27080.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 174174CytoplasmicSequence analysisAdd
BLAST
Transmembranei175 – 19218HelicalSequence analysisAdd
BLAST
Topological domaini193 – 20210Mitochondrial intermembraneSequence analysis

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: TAIR
  • mitochondrial inner membrane presequence translocase complex Source: TAIR
  • mitochondrial outer membrane Source: TAIR
  • mitochondrial outer membrane translocase complex Source: TAIR
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Triple mutants tom20-2-tom20-3-tom20-4 are vible but display a slightly delayed flowering time.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Mitochondrial import receptor subunit TOM20-3PRO_0000051545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineCombined sources

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP82874.
PRIDEiP82874.

PTM databases

iPTMnetiP82874.
SwissPalmiP82874.

Expressioni

Tissue specificityi

Expressed in roots, flowers, young cotyledons and leaves.1 Publication

Gene expression databases

GenevisibleiP82874. AT.

Interactioni

Subunit structurei

Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 6 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22/TOM9 and TOM40).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NAC089Q94F582EBI-2352074,EBI-2319707

Protein-protein interaction databases

BioGridi7656. 11 interactions.
IntActiP82874. 15 interactions.
STRINGi3702.AT3G27080.1.

Structurei

Secondary structure

1
202
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2620Combined sources
Helixi31 – 4717Combined sources
Helixi50 – 7021Combined sources
Helixi75 – 9117Combined sources
Helixi95 – 11521Combined sources
Helixi120 – 13011Combined sources
Helixi132 – 14110Combined sources
Beta strandi142 – 1443Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZU2NMR-A1-145[»]
ProteinModelPortaliP82874.
SMRiP82874. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82874.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati38 – 7437TPR 1Add
BLAST
Repeati86 – 11934TPR 2Add
BLAST

Sequence similaritiesi

Belongs to the Tom20 family.Curated
Contains 2 TPR repeats.Curated

Keywords - Domaini

Repeat, TPR repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IKS7. Eukaryota.
ENOG4111JA8. LUCA.
HOGENOMiHOG000005870.
InParanoidiP82874.
OMAiIWCLGNA.
OrthoDBiEOG09360NVP.
PhylomeDBiP82874.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR010547. TOM20_imprt_rcpt.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF06552. TOM20_plant. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.

Sequencei

Sequence statusi: Complete.

P82874-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTETEFDRI LLFEQIRQDA ENTYKSNPLD ADNLTRWGGV LLELSQFHSI
60 70 80 90 100
SDAKQMIQEA ITKFEEALLI DPKKDEAVWC IGNAYTSFAF LTPDETEAKH
110 120 130 140 150
NFDLATQFFQ QAVDEQPDNT HYLKSLEMTA KAPQLHAEAY KQGLGSQPMG
160 170 180 190 200
RVEAPAPPSS KAVKNKKSSD AKYDAMGWVI LAIGVVAWIS FAKANVPVSP

PR
Length:202
Mass (Da):22,565
Last modified:March 1, 2001 - v1
Checksum:iF86A54F827F3A6D1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti12 – 121L → F in AAM64598 (Ref. 5) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ296025 mRNA. Translation: CAC14430.1.
AB026649 Genomic DNA. Translation: BAB01089.1.
CP002686 Genomic DNA. Translation: AEE77264.1.
AY040027 mRNA. Translation: AAK64184.1.
AY079411 mRNA. Translation: AAL85142.1.
AY087037 mRNA. Translation: AAM64598.1.
RefSeqiNP_189344.1. NM_113622.3.
UniGeneiAt.6114.

Genome annotation databases

EnsemblPlantsiAT3G27080.1; AT3G27080.1; AT3G27080.
GeneIDi822326.
GrameneiAT3G27080.1; AT3G27080.1; AT3G27080.
KEGGiath:AT3G27080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ296025 mRNA. Translation: CAC14430.1.
AB026649 Genomic DNA. Translation: BAB01089.1.
CP002686 Genomic DNA. Translation: AEE77264.1.
AY040027 mRNA. Translation: AAK64184.1.
AY079411 mRNA. Translation: AAL85142.1.
AY087037 mRNA. Translation: AAM64598.1.
RefSeqiNP_189344.1. NM_113622.3.
UniGeneiAt.6114.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZU2NMR-A1-145[»]
ProteinModelPortaliP82874.
SMRiP82874. Positions 1-145.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi7656. 11 interactions.
IntActiP82874. 15 interactions.
STRINGi3702.AT3G27080.1.

PTM databases

iPTMnetiP82874.
SwissPalmiP82874.

Proteomic databases

PaxDbiP82874.
PRIDEiP82874.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G27080.1; AT3G27080.1; AT3G27080.
GeneIDi822326.
GrameneiAT3G27080.1; AT3G27080.1; AT3G27080.
KEGGiath:AT3G27080.

Organism-specific databases

TAIRiAT3G27080.

Phylogenomic databases

eggNOGiENOG410IKS7. Eukaryota.
ENOG4111JA8. LUCA.
HOGENOMiHOG000005870.
InParanoidiP82874.
OMAiIWCLGNA.
OrthoDBiEOG09360NVP.
PhylomeDBiP82874.

Miscellaneous databases

EvolutionaryTraceiP82874.
PROiP82874.

Gene expression databases

GenevisibleiP82874. AT.

Family and domain databases

Gene3Di1.25.40.10. 1 hit.
InterProiIPR010547. TOM20_imprt_rcpt.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF06552. TOM20_plant. 1 hit.
[Graphical view]
SUPFAMiSSF48452. SSF48452. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTO203_ARATH
AccessioniPrimary (citable) accession number: P82874
Secondary accession number(s): Q8LBR8, Q9LSC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 1, 2001
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

There are four genes (TOM20-1, TOM20-2, TOM20-3 and TOM20-4) which encode mitochondrial import receptor subunits TOM20.
In mammals and fungi, the transmembrane domain is located at the N-terminus while it is located at the C-terminus in plants. The overall orientation of the protein in the membrane is therefore inverted.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.