Mitochondrial import receptor subunit TOM20-3 - Arabidopsis thaliana (Mouse-ear cress)

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P82874 (TO203_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 85. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Mitochondrial import receptor subunit TOM20-3

Alternative name(s):
Translocase of outer membrane 20 kDa subunit 3

Gene names

Name:	TOM20-3
Ordered Locus Names:	At3g27080
ORF Names:	MOJ10.15

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Central component of the receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the translocation pore.
Subunit structure	Forms part of the preprotein translocase complex of the outer mitochondrial membrane (TOM complex) which consists of at least 6 different proteins (TOM5, TOM6, TOM7, TOM20, TOM22/TOM9 and TOM40). Ref.6
Subcellular location	Mitochondrion outer membrane; Single-pass membrane protein Potential.
Post-translational modification	The N-terminus is blocked.
Miscellaneous	There are four genes (TOM20-1, TOM20-2, TOM20-3 and TOM20-4) which encode mitochondrial import receptor subunits TOM20. In mammals and fungi, the transmembrane domain is located at the N-terminus while it is located at the C-terminus in plants. The overall orientation of the protein in the membrane is therefore inverted.
Sequence similarities	Belongs to the Tom20 family. Contains 2 TPR repeats.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Repeat TPR repeat Transmembrane Transmembrane helix
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	protein import into mitochondrial outer membrane Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane presequence translocase complex Traceable author statement Ref.1. Source: TAIR mitochondrial outer membrane translocase complex Inferred from direct assay Ref.1. Source: TAIR
Molecular function	P-P-bond-hydrolysis-driven protein transmembrane transporter activity Inferred from direct assay Ref.7. Source: TAIR protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NAC089	Q94F58	2	EBI-2352074,EBI-2319707

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 202

202

Mitochondrial import receptor subunit TOM20-3

PRO_0000051545

Regions

Topological domain

1 – 174

174

Cytoplasmic Potential

Transmembrane

175 – 192

Helical; Potential

Topological domain

193 – 202

Mitochondrial intermembrane Potential

Repeat

38 – 74

TPR 1

Repeat

86 – 119

TPR 2

Experimental info

Sequence conflict

L → F in AAM64598. Ref.5

Secondary structure

202

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P82874 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: F86A54F827F3A6D1
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FASTA

202

22,565

        10         20         30         40         50         60 
MDTETEFDRI LLFEQIRQDA ENTYKSNPLD ADNLTRWGGV LLELSQFHSI SDAKQMIQEA 

        70         80         90        100        110        120 
ITKFEEALLI DPKKDEAVWC IGNAYTSFAF LTPDETEAKH NFDLATQFFQ QAVDEQPDNT 

       130        140        150        160        170        180 
HYLKSLEMTA KAPQLHAEAY KQGLGSQPMG RVEAPAPPSS KAVKNKKSSD AKYDAMGWVI 

       190        200 
LAIGVVAWIS FAKANVPVSP PR

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification and characterization of the preprotein translocase of the outer mitochondrial membrane from Arabidopsis. Identification of multiple forms of TOM20." Werhahn W., Niemeyer A., Jaensch L., Kruft V., Schmitz U.K., Braun H.-P. Plant Physiol. 125:943-954(2001) [PubMed: 11161051] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 99-123. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]	"Identification of novel subunits of the TOM complex from Arabidopsis thaliana." Werhahn W., Jaensch L., Braun H.-P. Plant Physiol. Biochem. 41:407-416(2003) Cited for: PROTEIN SEQUENCE OF 4-5, SUBUNIT.
[7]	"Convergent evolution of receptors for protein import into mitochondria." Perry A.J., Hulett J.M., Likic V.A., Lithgow T., Gooley P.R. Curr. Biol. 16:221-229(2006) [PubMed: 16461275] [Abstract] Cited for: STRUCTURE BY NMR OF 1-145.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AJ296025 mRNA. Translation: CAC14430.1.
AB026649 Genomic DNA. Translation: BAB01089.1.
CP002686 Genomic DNA. Translation: AEE77264.1.
AY040027 mRNA. Translation: AAK64184.1.
AY079411 mRNA. Translation: AAL85142.1.
AY087037 mRNA. Translation: AAM64598.1.

IPI

IPI00528881.

RefSeq

NP_189344.1. NM_113622.3.

UniGene

At.6114.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ZU2	NMR	-	A	1-145	[»]

ProteinModelPortal

P82874.

SMR

P82874. Positions 1-145.

ModBase

Search...

Protein-protein interaction databases

IntAct

P82874. 15 interactions.

STRING

P82874.

Proteomic databases

PRIDE

P82874.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblPlants

AT3G27080.1; AT3G27080.1; AT3G27080.

GeneID

822326.

GenomeReviews

Gene locus AT3G27080 in contig BA000014_GR.

KEGG

ath:AT3G27080.

NMPDR

fig|3702.1.peg.14995.

Organism-specific databases

GeneFarm

2106. 167.

TAIR

At3g27080.

Phylogenomic databases

GeneTree

EPGT00050000016952.

HOGENOM

HBG317717.

InParanoid

P82874.

OMA

LELAHFR.

PhylomeDB

P82874.

ProtClustDB

CLSN2684989.

Gene expression databases

ArrayExpress

P82874.

Genevestigator

P82874.

GermOnline

AT3G27080. Arabidopsis thaliana.

Family and domain databases

InterPro

IPR008940. Prenyltransferase.
IPR010547. TOM20_imprt_rcpt.
[Graphical view]

Gene3D

G3DSA:1.25.40.120. Prenyl_trans. 1 hit.

Pfam

PF06552. TOM20_plant. 1 hit.
[Graphical view]

PROSITE

PS50005. TPR. False negative.
PS50293. TPR_REGION. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TO203_ARATH

Accession

Primary (citable) accession number: P82874
Secondary accession number(s): Q8LBR8, Q9LSC8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	March 1, 2001
Last modified:	January 25, 2012
This is version 85 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents