Reviewed, UniProtKB/Swiss-Prot P82858 (URIC_PUCRE)

Names and origin

Protein names	Recommended name:     Uricase     EC=1.7.3.3 Alternative name(s):     Urate oxidase
Organism	Puccinia recondita f. sp. triseti
Taxonomic identifier	142679 [NCBI]
Taxonomic lineage	Eukaryota › Fungi › Dikarya › Basidiomycota › Pucciniomycotina › Pucciniomycetes › Pucciniales › Pucciniaceae › Puccinia

Protein attributes

Sequence length	8 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin. Ref.1
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2. Ref.1
Enzyme regulation	Inhibited by two structural analogs of urate, oxonate and xanthine. Ref.1
Pathway	Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3.
Subunit structure	Heterotetramer. Ref.1
Sequence similarities	Belongs to the uricase family. Ref.1
biophysicochemical properties	Kinetic parameters: KM=35 µM for Urate Ref.1 pH dependence: Optimum pH is 9.0. Ref.1

Ontologies

Keywords
Biological process	Purine metabolism
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW purine base metabolic process Ref.1 Inferred from direct assay. Source: UniProtKB
Molecular function	urate oxidase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›8	›8	Uricase		PRO_0000262767
Experimental info
	Non-terminal residue	8	1

Sequences

	Sequence		Length	Mass (Da)
	P82858-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 98C1ADD735B9D76D	FASTA	8	777
	APFSLATA

References

[1]

"Urate oxidase from the rust Puccinia recondita is a heterotetramer with two different-sized monomers." Aguilar M., Montalbini P., Pineda M. Curr. Microbiol. 44:257-261(2002) [PubMed: 11910495] [Abstract] Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT. Tissue: Spore.

Cross-references

3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR019842. Uricase_CS. [Graphical view]
PROSITE	PS00366. URICASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC_PUCRE

Accession

Primary (citable) accession number: P82858

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)