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P82850 (KKX11_HETFU) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Potassium channel toxin kappa-KTx 1.1
Alternative name(s):
Kappa-hefutoxin-1
Short name=Kappa-HfTx1
OrganismHeterometrus fulvipes (Indian black scorpion)
Taxonomic identifier141248 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaScorpionesIuridaScorpionoideaScorpionidaeScorpioninaeHeterometrus

Protein attributes

Sequence length22 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Slows the activation kinetics of Kv1.3/KCNA3 currents, and blocks Kv1.3/KCNA3 and Kv1.2/KCNA2 potassium channels. This block is dose-dependent, voltage-independent, and reversible. Ref.1

Subcellular location

Secreted.

Tissue specificity

Expressed by the venom gland.

Domain

Has the structural arrangement of two alpha-helices stabilized by two disulfide bonds (CSalpha/alpha).

Miscellaneous

This toxin does not have effect on Kv1.1/KCNA1 currents (Ref.1).

Sequence similarities

Belongs to the short scorpion toxin superfamily. Potassium channel inhibitor kappa-KTx family. Kappa-KTx 1 subfamily.

Mass spectrometry

Molecular mass is 2655.4±0.2 Da from positions 1 - 22. Determined by ESI. Ref.1

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Peptide1 – 2222Potassium channel toxin kappa-KTx 1.1
PRO_0000044544

Amino acid modifications

Modified residue221Cysteine amide
Disulfide bond4 ↔ 22 Ref.1
Disulfide bond8 ↔ 18 Ref.1

Experimental info

Mutagenesis51Y → A: Loss of channels blocking. Same effect observed; when associated with A-19. Ref.1
Mutagenesis191K → A: Loss of channels blocking. Same effect observed; when associated with A-5. Ref.1

Secondary structure

...... 22
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P82850 [UniParc].

Last modified March 1, 2001. Version 1.
Checksum: 8FB555EB877C6E98

FASTA222,660
        10         20 
GHACYRNCWR EGNDEETCKE RC 

« Hide

References

[1]"Kappa-hefutoxin1, a novel toxin from the scorpion Heterometrus fulvipes with unique structure and function. Importance of the functional diad in potassium channel selectivity."
Srinivasan K.N., Sivaraja V., Huys I., Sasaki T., Cheng B., Kumar T.K.S., Sato K., Tytgat J., Yu C., San B.C.C., Ranganathan S., Bowie H.J., Kini R.M., Gopalakrishnakone P.
J. Biol. Chem. 277:30040-30047(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE, FUNCTION, AMIDATION, STRUCTURE BY NMR, DISULFIDE BONDS, MASS SPECTROMETRY, MUTAGENESIS OF TYR-5; LYS-19 AND 5-TYR--LYS-19.
Tissue: Venom.

Cross-references

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1HP9NMR-A1-22[»]
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB8.B.2.1.1. the short scorpion toxin (sst) superfamily.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR012630. Toxin_25.
[Graphical view]
PfamPF08095. Toxin_25. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP82850.

Entry information

Entry nameKKX11_HETFU
AccessionPrimary (citable) accession number: P82850
Entry history
Integrated into UniProtKB/Swiss-Prot: May 4, 2001
Last sequence update: March 1, 2001
Last modified: October 16, 2013
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Scorpion potassium channel toxins

Nomenclature of scorpion potassium channel toxins and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references