Reviewed, UniProtKB/Swiss-Prot P82809 (AK1CD_MESAU)

Names and origin

Protein names

Recommended name:
    Aldo-keto reductase family 1 member C13
    EC=1.1.1.-
Alternative name(s):
    Morphine 6-dehydrogenase
    EC=1.1.1.218

Gene names

Name:

AKR1C13

Organism

Mesocricetus auratus (Golden hamster)

Taxonomic identifier

10036 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the dehydrogenation of morphine to morphinone. The enzyme also exhibits significant activity for a variety of cyclic and alicyclic alcohols. In addition to xenobiotics, the enzyme catalyzes the dehydrogenation of 17-beta-hydroxysteroids with much higher affinities than morphine. Uses both NAD and NADP, but the activity is much greater with NAD than with NADP.
Catalytic activity	Morphine + NAD(P)+ = morphinone + NAD(P)H.
Enzyme regulation	Strongly inhibited by sulfhydryl reagents and ketamine, but not by pyrazole, barbital and indomethacine.
Subunit structure	Monomer.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the aldo/keto reductase family.
Biophysicochemical properties	pH dependence: Optimum pH is 9.3 with NAD as the cofactor.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	morphine 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	Aldo-keto reductase family 1 member C13		PRO_0000124643
Regions
	Nucleotide binding	13 – 22	10	NADP Potential
	Nucleotide binding	217 – 280	64	NADP By similarity
Sites
	Active site	55	1	Proton donor By similarity
	Binding site	117	1	Substrate By similarity
	Site	84	1	Lowers pKa of active site Tyr By similarity

Sequences

	Sequence		Length	Mass (Da)
	P82809-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 0CFC67A8E37D2685	FASTA	322	36,507
	10 20 30 40 50 60 XXXXXXXXXX XDGHCIPALG FGTYKPIEVP KSKAMEAANL AIGVGYRHID TAYAYQIEEE 70 80 90 100 110 120 IGQAIQSNIK AGIVKREDMF ITTKLWCTCF QPELVRPSLE KXXXKLQLEH VDLFIMHYPV 130 140 150 160 170 180 PMKAGDNDFP LDEQGKLLLD TVDFCATWEA LEKXXDAGLV KSIGVSNFNM RQLERILNKP 190 200 210 220 230 240 GLKYKPVCNQ VECHVYNNQS KLLDYCKSKD IVLVAFGALG TQRYKEWVDQ DSPVLLNDPV 250 260 270 280 290 300 LCGGAKXXXR SPALIALRYL VQRGVVPLAQ SFYESEMKEN LQVFEFQLSP EDMKILDGLN 310 320 KNFRYLPAQF FADHPEYPFS EE

References

[1]

"Purification and characterization of NAD-dependent morphine 6-dehydrogenase from hamster liver cytosol, a new member of the aldo-keto reductase superfamily." Todaka T., Yamano S., Toki S. Arch. Biochem. Biophys. 374:189-197(2000) [PubMed: 10666297] [Abstract] Cited for: PROTEIN SEQUENCE, CHARACTERIZATION. Tissue: Liver.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1J96 based on UniProtKB P52895.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P82809.
Enzyme and pathway databases
BRENDA	1.1.1.218. 824.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AK1CD_MESAU

Accession

Primary (citable) accession number: P82809

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)