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P82808

- GFPT1_RAT

UniProt

P82808 - GFPT1_RAT

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene

Gfpt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.1 Publication

Enzyme regulationi

Inhibited by 4,4'-dithiodipyridine.1 Publication

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei426 – 4261SubstrateBy similarity
Binding sitei566 – 5661SubstrateBy similarity

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. carbohydrate binding Source: RGD
  3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: RGD

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. cellular response to insulin stimulus Source: RGD
  3. circadian regulation of gene expression Source: UniProtKB
  4. fructose 6-phosphate metabolic process Source: RGD
  5. glucosamine biosynthetic process Source: RGD
  6. glutamine metabolic process Source: UniProtKB-KW
  7. negative regulation of glycogen biosynthetic process Source: RGD
  8. protein homotetramerization Source: RGD
  9. response to insulin Source: RGD
  10. response to sucrose Source: RGD
  11. UDP-N-acetylglucosamine metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biological rhythms

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13170.
ReactomeiREACT_196379. Synthesis of UDP-N-acetyl-glucosamine.
REACT_93415. XBP1(S) activates chaperone genes.
SABIO-RKP82808.

Protein family/group databases

MEROPSiC44.970.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 1
Glutamine:fructose-6-phosphate amidotransferase 1
Short name:
GFAT 1
Short name:
GFAT1
Hexosephosphate aminotransferase 1
Gene namesi
Name:Gfpt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1549703. Gfpt1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 681680Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1PRO_0000135282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei243 – 2431PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP82808.
PRIDEiP82808.

PTM databases

PhosphoSiteiP82808.

Expressioni

Gene expression databases

GenevestigatoriP82808.

Interactioni

Subunit structurei

Homotetramer, may also exist as homodimers.By similarity

Protein-protein interaction databases

BioGridi255576. 1 interaction.
MINTiMINT-4577107.
STRINGi10116.ENSRNOP00000025078.

Structurei

3D structure databases

ProteinModelPortaliP82808.
SMRiP82808. Positions 321-669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 287286Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini359 – 498140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini530 – 671142SIS 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni295 – 662368IsomeraseBy similarityAdd
BLAST
Regioni376 – 3772Substrate-bindingBy similarity
Regioni421 – 4233Substrate-bindingBy similarity

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiP82808.
KOiK00820.
OMAiMYDNRDS.
OrthoDBiEOG73NG2S.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82808-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW
60 70 80 90 100
EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG
110 120 130 140 150
EPSPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE
160 170 180 190 200
TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG
210 220 230 240 250
TRRGSPLLIG VRSEHKLSTD HIPILYRTGK DKKGSCGLSR VDSTTCLFPV
260 270 280 290 300
EEKAVEYYFA SDASAVIEHT NRVIFLEDDD VAAVVDGRLS IHRIKRTARD
310 320 330 340 350
HPGRAVQTLQ MELQQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT
360 370 380 390 400
VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GMATRQVLEE LTELPVMVEL
410 420 430 440 450
ASDFLDRNTP VFRDDVCFFI SQSGETADTL MGLRYCKERG ALTVGITNTV
460 470 480 490 500
GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ
510 520 530 540 550
ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA
560 570 580 590 600
TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA
610 620 630 640 650
KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV
660 670 680
IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E
Length:681
Mass (Da):76,827
Last modified:January 23, 2007 - v3
Checksum:i230D818CBC920249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083889 mRNA. Translation: AAH83889.1.
RefSeqiNP_001005879.1. NM_001005879.1.
UniGeneiRn.229622.

Genome annotation databases

EnsembliENSRNOT00000025070; ENSRNOP00000025070; ENSRNOG00000018507.
GeneIDi297417.
KEGGirno:297417.
UCSCiRGD:1549703. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083889 mRNA. Translation: AAH83889.1 .
RefSeqi NP_001005879.1. NM_001005879.1.
UniGenei Rn.229622.

3D structure databases

ProteinModelPortali P82808.
SMRi P82808. Positions 321-669.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 255576. 1 interaction.
MINTi MINT-4577107.
STRINGi 10116.ENSRNOP00000025078.

Protein family/group databases

MEROPSi C44.970.

PTM databases

PhosphoSitei P82808.

Proteomic databases

PaxDbi P82808.
PRIDEi P82808.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025070 ; ENSRNOP00000025070 ; ENSRNOG00000018507 .
GeneIDi 297417.
KEGGi rno:297417.
UCSCi RGD:1549703. rat.

Organism-specific databases

CTDi 2673.
RGDi 1549703. Gfpt1.

Phylogenomic databases

eggNOGi COG0449.
GeneTreei ENSGT00390000010049.
HOGENOMi HOG000258898.
HOVERGENi HBG051724.
InParanoidi P82808.
KOi K00820.
OMAi MYDNRDS.
OrthoDBi EOG73NG2S.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13170.
Reactomei REACT_196379. Synthesis of UDP-N-acetyl-glucosamine.
REACT_93415. XBP1(S) activates chaperone genes.
SABIO-RK P82808.

Miscellaneous databases

NextBioi 642231.
PROi P82808.

Gene expression databases

Genevestigatori P82808.

Family and domain databases

Gene3Di 3.60.20.10. 2 hits.
InterProi IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
Pfami PF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 2 hits.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Purification and characterization of glutamine:fructose 6-phosphate amidotransferase from rat liver."
    Huynh Q.K., Gulve E.A., Dian T.
    Arch. Biochem. Biophys. 379:307-313(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    Strain: Sprague-Dawley1 Publication.
    Tissue: Liver1 Publication.

Entry informationi

Entry nameiGFPT1_RAT
AccessioniPrimary (citable) accession number: P82808
Secondary accession number(s): Q5XI08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 90 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3