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P82808

- GFPT1_RAT

UniProt

P82808 - GFPT1_RAT

Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene

Gfpt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 89 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

    Catalytic activityi

    L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.1 Publication

    Enzyme regulationi

    Inhibited by 4,4'-dithiodipyridine.1 Publication

    pH dependencei

    Optimum pH is 7.5.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei2 – 21For GATase activityBy similarity
    Binding sitei426 – 4261SubstrateBy similarity
    Binding sitei566 – 5661SubstrateBy similarity

    GO - Molecular functioni

    1. amino acid binding Source: RGD
    2. carbohydrate binding Source: RGD
    3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: RGD

    GO - Biological processi

    1. carbohydrate biosynthetic process Source: InterPro
    2. cellular response to insulin stimulus Source: RGD
    3. circadian regulation of gene expression Source: UniProtKB
    4. fructose 6-phosphate metabolic process Source: RGD
    5. glucosamine biosynthetic process Source: RGD
    6. glutamine metabolic process Source: UniProtKB-KW
    7. negative regulation of glycogen biosynthetic process Source: RGD
    8. protein homotetramerization Source: RGD
    9. response to insulin Source: RGD
    10. response to sucrose Source: RGD
    11. UDP-N-acetylglucosamine metabolic process Source: RGD

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biological rhythms

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-13170.
    ReactomeiREACT_196379. Synthesis of UDP-N-acetyl-glucosamine.
    REACT_93415. XBP1(S) activates chaperone genes.
    SABIO-RKP82808.

    Protein family/group databases

    MEROPSiC44.970.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16)
    Alternative name(s):
    D-fructose-6-phosphate amidotransferase 1
    Glutamine:fructose-6-phosphate amidotransferase 1
    Short name:
    GFAT 1
    Short name:
    GFAT1
    Hexosephosphate aminotransferase 1
    Gene namesi
    Name:Gfpt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 4

    Organism-specific databases

    RGDi1549703. Gfpt1.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 681680Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1PRO_0000135282Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei243 – 2431PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP82808.
    PRIDEiP82808.

    PTM databases

    PhosphoSiteiP82808.

    Expressioni

    Gene expression databases

    GenevestigatoriP82808.

    Interactioni

    Subunit structurei

    Homotetramer, may also exist as homodimers.By similarity

    Protein-protein interaction databases

    MINTiMINT-4577107.
    STRINGi10116.ENSRNOP00000025078.

    Structurei

    3D structure databases

    ProteinModelPortaliP82808.
    SMRiP82808. Positions 321-669.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 287286Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini359 – 498140SIS 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini530 – 671142SIS 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni295 – 662368IsomeraseBy similarityAdd
    BLAST
    Regioni376 – 3772Substrate-bindingBy similarity
    Regioni421 – 4233Substrate-bindingBy similarity

    Sequence similaritiesi

    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
    Contains 2 SIS domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Repeat

    Phylogenomic databases

    eggNOGiCOG0449.
    GeneTreeiENSGT00390000010049.
    HOGENOMiHOG000258898.
    HOVERGENiHBG051724.
    KOiK00820.
    OMAiMYDNRDS.
    OrthoDBiEOG73NG2S.

    Family and domain databases

    Gene3Di3.60.20.10. 2 hits.
    InterProiIPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view]
    PfamiPF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view]
    SUPFAMiSSF56235. SSF56235. 2 hits.
    TIGRFAMsiTIGR01135. glmS. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P82808-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW    50
    EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG 100
    EPSPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE 150
    TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG 200
    TRRGSPLLIG VRSEHKLSTD HIPILYRTGK DKKGSCGLSR VDSTTCLFPV 250
    EEKAVEYYFA SDASAVIEHT NRVIFLEDDD VAAVVDGRLS IHRIKRTARD 300
    HPGRAVQTLQ MELQQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT 350
    VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GMATRQVLEE LTELPVMVEL 400
    ASDFLDRNTP VFRDDVCFFI SQSGETADTL MGLRYCKERG ALTVGITNTV 450
    GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ 500
    ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA 550
    TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA 600
    KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV 650
    IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E 681
    Length:681
    Mass (Da):76,827
    Last modified:January 23, 2007 - v3
    Checksum:i230D818CBC920249
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC083889 mRNA. Translation: AAH83889.1.
    RefSeqiNP_001005879.1. NM_001005879.1.
    UniGeneiRn.229622.

    Genome annotation databases

    EnsembliENSRNOT00000025070; ENSRNOP00000025070; ENSRNOG00000018507.
    GeneIDi297417.
    KEGGirno:297417.
    UCSCiRGD:1549703. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BC083889 mRNA. Translation: AAH83889.1 .
    RefSeqi NP_001005879.1. NM_001005879.1.
    UniGenei Rn.229622.

    3D structure databases

    ProteinModelPortali P82808.
    SMRi P82808. Positions 321-669.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4577107.
    STRINGi 10116.ENSRNOP00000025078.

    Protein family/group databases

    MEROPSi C44.970.

    PTM databases

    PhosphoSitei P82808.

    Proteomic databases

    PaxDbi P82808.
    PRIDEi P82808.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025070 ; ENSRNOP00000025070 ; ENSRNOG00000018507 .
    GeneIDi 297417.
    KEGGi rno:297417.
    UCSCi RGD:1549703. rat.

    Organism-specific databases

    CTDi 2673.
    RGDi 1549703. Gfpt1.

    Phylogenomic databases

    eggNOGi COG0449.
    GeneTreei ENSGT00390000010049.
    HOGENOMi HOG000258898.
    HOVERGENi HBG051724.
    KOi K00820.
    OMAi MYDNRDS.
    OrthoDBi EOG73NG2S.

    Enzyme and pathway databases

    BioCyci MetaCyc:MONOMER-13170.
    Reactomei REACT_196379. Synthesis of UDP-N-acetyl-glucosamine.
    REACT_93415. XBP1(S) activates chaperone genes.
    SABIO-RK P82808.

    Miscellaneous databases

    NextBioi 642231.
    PROi P82808.

    Gene expression databases

    Genevestigatori P82808.

    Family and domain databases

    Gene3Di 3.60.20.10. 2 hits.
    InterProi IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR005855. GlmS_trans.
    IPR029055. Ntn_hydrolases_N.
    IPR001347. SIS.
    [Graphical view ]
    Pfami PF00310. GATase_2. 2 hits.
    PF01380. SIS. 2 hits.
    [Graphical view ]
    SUPFAMi SSF56235. SSF56235. 2 hits.
    TIGRFAMsi TIGR01135. glmS. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS51464. SIS. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.
    2. "Purification and characterization of glutamine:fructose 6-phosphate amidotransferase from rat liver."
      Huynh Q.K., Gulve E.A., Dian T.
      Arch. Biochem. Biophys. 379:307-313(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-14, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
      Strain: Sprague-Dawley1 Publication.
      Tissue: Liver1 Publication.

    Entry informationi

    Entry nameiGFPT1_RAT
    AccessioniPrimary (citable) accession number: P82808
    Secondary accession number(s): Q5XI08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 31, 2004
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 89 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3