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P82808

- GFPT1_RAT

UniProt

P82808 - GFPT1_RAT

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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene
Gfpt1
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.1 Publication

Enzyme regulationi

Inhibited by 4,4'-dithiodipyridine.1 Publication

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activity By similarityBy similarity
Binding sitei426 – 4261Substrate By similarity
Binding sitei566 – 5661Substrate By similarity

GO - Molecular functioni

  1. amino acid binding Source: RGD
  2. carbohydrate binding Source: RGD
  3. glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: RGD

GO - Biological processi

  1. carbohydrate biosynthetic process Source: InterPro
  2. cellular response to insulin stimulus Source: RGD
  3. circadian regulation of gene expression Source: UniProtKB
  4. fructose 6-phosphate metabolic process Source: RGD
  5. glucosamine biosynthetic process Source: RGD
  6. glutamine metabolic process Source: UniProtKB-KW
  7. negative regulation of glycogen biosynthetic process Source: RGD
  8. protein homotetramerization Source: RGD
  9. response to insulin Source: RGD
  10. response to sucrose Source: RGD
  11. UDP-N-acetylglucosamine metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biological rhythms

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13170.
ReactomeiREACT_196379. Synthesis of UDP-N-acetyl-glucosamine.
REACT_93415. XBP1(S) activates chaperone genes.
SABIO-RKP82808.

Protein family/group databases

MEROPSiC44.970.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 1
Glutamine:fructose-6-phosphate amidotransferase 1
Short name:
GFAT 1
Short name:
GFAT1
Hexosephosphate aminotransferase 1
Gene namesi
Name:Gfpt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi1549703. Gfpt1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity1 Publication
Chaini2 – 681680Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1PRO_0000135282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei243 – 2431Phosphoserine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP82808.
PRIDEiP82808.

PTM databases

PhosphoSiteiP82808.

Expressioni

Gene expression databases

GenevestigatoriP82808.

Interactioni

Subunit structurei

Homotetramer, may also exist as homodimers By similarity.

Protein-protein interaction databases

MINTiMINT-4577107.
STRINGi10116.ENSRNOP00000025078.

Structurei

3D structure databases

ProteinModelPortaliP82808.
SMRiP82808. Positions 321-669.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 287286Glutamine amidotransferase type-2Add
BLAST
Domaini359 – 498140SIS 1Add
BLAST
Domaini530 – 671142SIS 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni295 – 662368Isomerase By similarityAdd
BLAST
Regioni376 – 3772Substrate-binding By similarity
Regioni421 – 4233Substrate-binding By similarity

Sequence similaritiesi

Contains 2 SIS domains.

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
KOiK00820.
OMAiMYDNRDS.
OrthoDBiEOG73NG2S.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82808-1 [UniParc]FASTAAdd to Basket

« Hide

MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW    50
EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG 100
EPSPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE 150
TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG 200
TRRGSPLLIG VRSEHKLSTD HIPILYRTGK DKKGSCGLSR VDSTTCLFPV 250
EEKAVEYYFA SDASAVIEHT NRVIFLEDDD VAAVVDGRLS IHRIKRTARD 300
HPGRAVQTLQ MELQQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT 350
VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GMATRQVLEE LTELPVMVEL 400
ASDFLDRNTP VFRDDVCFFI SQSGETADTL MGLRYCKERG ALTVGITNTV 450
GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ 500
ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA 550
TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA 600
KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV 650
IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E 681
Length:681
Mass (Da):76,827
Last modified:January 23, 2007 - v3
Checksum:i230D818CBC920249
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC083889 mRNA. Translation: AAH83889.1.
RefSeqiNP_001005879.1. NM_001005879.1.
UniGeneiRn.229622.

Genome annotation databases

EnsembliENSRNOT00000025070; ENSRNOP00000025070; ENSRNOG00000018507.
GeneIDi297417.
KEGGirno:297417.
UCSCiRGD:1549703. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BC083889 mRNA. Translation: AAH83889.1 .
RefSeqi NP_001005879.1. NM_001005879.1.
UniGenei Rn.229622.

3D structure databases

ProteinModelPortali P82808.
SMRi P82808. Positions 321-669.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4577107.
STRINGi 10116.ENSRNOP00000025078.

Protein family/group databases

MEROPSi C44.970.

PTM databases

PhosphoSitei P82808.

Proteomic databases

PaxDbi P82808.
PRIDEi P82808.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025070 ; ENSRNOP00000025070 ; ENSRNOG00000018507 .
GeneIDi 297417.
KEGGi rno:297417.
UCSCi RGD:1549703. rat.

Organism-specific databases

CTDi 2673.
RGDi 1549703. Gfpt1.

Phylogenomic databases

eggNOGi COG0449.
GeneTreei ENSGT00390000010049.
HOGENOMi HOG000258898.
HOVERGENi HBG051724.
KOi K00820.
OMAi MYDNRDS.
OrthoDBi EOG73NG2S.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER-13170.
Reactomei REACT_196379. Synthesis of UDP-N-acetyl-glucosamine.
REACT_93415. XBP1(S) activates chaperone genes.
SABIO-RK P82808.

Miscellaneous databases

NextBioi 642231.
PROi P82808.

Gene expression databases

Genevestigatori P82808.

Family and domain databases

Gene3Di 3.60.20.10. 2 hits.
InterProi IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view ]
Pfami PF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view ]
SUPFAMi SSF56235. SSF56235. 2 hits.
TIGRFAMsi TIGR01135. glmS. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Purification and characterization of glutamine:fructose 6-phosphate amidotransferase from rat liver."
    Huynh Q.K., Gulve E.A., Dian T.
    Arch. Biochem. Biophys. 379:307-313(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    Strain: Sprague-Dawley.
    Tissue: Liver.

Entry informationi

Entry nameiGFPT1_RAT
AccessioniPrimary (citable) accession number: P82808
Secondary accession number(s): Q5XI08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: September 3, 2014
This is version 88 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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