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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1

Gene

Gfpt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Regulates the circadian expression of clock genes ARNTL/BMAL1 and CRY1.1 Publication

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.1 Publication

Enzyme regulationi

Inhibited by 4,4'-dithiodipyridine.1 Publication

pH dependencei

Optimum pH is 7.5.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei2 – 21For GATase activityBy similarity
Binding sitei426 – 4261SubstrateBy similarity
Binding sitei566 – 5661SubstrateBy similarity

GO - Molecular functioni

  • amino acid binding Source: RGD
  • carbohydrate binding Source: RGD
  • glutamine-fructose-6-phosphate transaminase (isomerizing) activity Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • cellular response to insulin stimulus Source: RGD
  • circadian regulation of gene expression Source: UniProtKB
  • fructose 6-phosphate metabolic process Source: RGD
  • glucosamine biosynthetic process Source: RGD
  • glutamine metabolic process Source: UniProtKB-KW
  • negative regulation of glycogen biosynthetic process Source: RGD
  • protein homotetramerization Source: RGD
  • response to insulin Source: RGD
  • response to sucrose Source: RGD
  • UDP-N-acetylglucosamine metabolic process Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biological rhythms

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13170.
ReactomeiREACT_287410. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKP82808.

Protein family/group databases

MEROPSiC44.970.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (EC:2.6.1.16)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 1
Glutamine:fructose-6-phosphate amidotransferase 1
Short name:
GFAT 1
Short name:
GFAT1
Hexosephosphate aminotransferase 1
Gene namesi
Name:Gfpt1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi1549703. Gfpt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 681680Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1PRO_0000135282Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei243 – 2431PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP82808.
PRIDEiP82808.

PTM databases

PhosphoSiteiP82808.

Expressioni

Gene expression databases

GenevisibleiP82808. RN.

Interactioni

Subunit structurei

Homotetramer, may also exist as homodimers.By similarity

Protein-protein interaction databases

BioGridi255576. 1 interaction.
MINTiMINT-4577107.
STRINGi10116.ENSRNOP00000025070.

Structurei

3D structure databases

ProteinModelPortaliP82808.
SMRiP82808. Positions 321-669.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 287286Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST
Domaini359 – 498140SIS 1PROSITE-ProRule annotationAdd
BLAST
Domaini530 – 671142SIS 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni295 – 662368IsomeraseBy similarityAdd
BLAST
Regioni376 – 3772Substrate-bindingBy similarity
Regioni421 – 4233Substrate-bindingBy similarity

Sequence similaritiesi

Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation
Contains 2 SIS domains.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiP82808.
KOiK00820.
OMAiIMRDNTY.
OrthoDBiEOG73NG2S.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82808-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAYLNY HVPRTRREIL ETLIKGLQRL EYRGYDSAGV GLDGGNDKDW
60 70 80 90 100
EANACKIQLI KKKGKVKALD EEVHKQQDMD LDIEFDVHLG IAHTRWATHG
110 120 130 140 150
EPSPVNSHPQ RSDKNNEFIV IHNGIITNYK DLKKFLESKG YDFESETDTE
160 170 180 190 200
TIAKLVKYMY DNWESQDVSF TTLVERVIQQ LEGAFALVFK SVHFPGQAVG
210 220 230 240 250
TRRGSPLLIG VRSEHKLSTD HIPILYRTGK DKKGSCGLSR VDSTTCLFPV
260 270 280 290 300
EEKAVEYYFA SDASAVIEHT NRVIFLEDDD VAAVVDGRLS IHRIKRTARD
310 320 330 340 350
HPGRAVQTLQ MELQQIMKGN FSSFMQKEIF EQPESVVNTM RGRVNFDDYT
360 370 380 390 400
VNLGGLKDHI KEIQRCRRLI LIACGTSYHA GMATRQVLEE LTELPVMVEL
410 420 430 440 450
ASDFLDRNTP VFRDDVCFFI SQSGETADTL MGLRYCKERG ALTVGITNTV
460 470 480 490 500
GSSISRETDC GVHINAGPEI GVASTKAYTS QFVSLVMFAL MMCDDRISMQ
510 520 530 540 550
ERRKEIMLGL KRLPDLIKEV LSMDDEIQKL ATELYHQKSV LIMGRGYHYA
560 570 580 590 600
TCLEGALKIK EITYMHSEGI LAGELKHGPL ALVDKLMPVI MIIMRDHTYA
610 620 630 640 650
KCQNALQQVV ARQGRPVVIC DKEDTETIKN TKRTIKVPHS VDCLQGILSV
660 670 680
IPLQLLAFHL AVLRGYDVDF PRNLAKSVTV E
Length:681
Mass (Da):76,827
Last modified:January 23, 2007 - v3
Checksum:i230D818CBC920249
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083889 mRNA. Translation: AAH83889.1.
RefSeqiNP_001005879.1. NM_001005879.1.
UniGeneiRn.229622.

Genome annotation databases

EnsembliENSRNOT00000025070; ENSRNOP00000025070; ENSRNOG00000018507.
GeneIDi297417.
KEGGirno:297417.
UCSCiRGD:1549703. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC083889 mRNA. Translation: AAH83889.1.
RefSeqiNP_001005879.1. NM_001005879.1.
UniGeneiRn.229622.

3D structure databases

ProteinModelPortaliP82808.
SMRiP82808. Positions 321-669.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi255576. 1 interaction.
MINTiMINT-4577107.
STRINGi10116.ENSRNOP00000025070.

Protein family/group databases

MEROPSiC44.970.

PTM databases

PhosphoSiteiP82808.

Proteomic databases

PaxDbiP82808.
PRIDEiP82808.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000025070; ENSRNOP00000025070; ENSRNOG00000018507.
GeneIDi297417.
KEGGirno:297417.
UCSCiRGD:1549703. rat.

Organism-specific databases

CTDi2673.
RGDi1549703. Gfpt1.

Phylogenomic databases

eggNOGiCOG0449.
GeneTreeiENSGT00390000010049.
HOGENOMiHOG000258898.
HOVERGENiHBG051724.
InParanoidiP82808.
KOiK00820.
OMAiIMRDNTY.
OrthoDBiEOG73NG2S.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13170.
ReactomeiREACT_287410. Synthesis of UDP-N-acetyl-glucosamine.
SABIO-RKP82808.

Miscellaneous databases

NextBioi642231.
PROiP82808.

Gene expression databases

GenevisibleiP82808. RN.

Family and domain databases

Gene3Di3.60.20.10. 2 hits.
InterProiIPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR005855. GlmS_trans.
IPR029055. Ntn_hydrolases_N.
IPR001347. SIS.
[Graphical view]
PfamiPF00310. GATase_2. 2 hits.
PF01380. SIS. 2 hits.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 2 hits.
TIGRFAMsiTIGR01135. glmS. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS51464. SIS. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.
  2. "Purification and characterization of glutamine:fructose 6-phosphate amidotransferase from rat liver."
    Huynh Q.K., Gulve E.A., Dian T.
    Arch. Biochem. Biophys. 379:307-313(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION.
    Strain: Sprague-Dawley1 Publication.
    Tissue: Liver1 Publication.

Entry informationi

Entry nameiGFPT1_RAT
AccessioniPrimary (citable) accession number: P82808
Secondary accession number(s): Q5XI08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 95 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.