Reviewed, UniProtKB/Swiss-Prot P82807 (FA10V_NOTSC)

Names and origin

Protein names	Recommended name:     Venom coagulation factor Xa-like protease     EC=3.4.21.6 Cleaved into the following 2 chains:     1- Recommended name:             Venom coagulation factor Xa-like protease light chain     2- Recommended name:             Venom coagulation factor Xa-like protease heavy chain
Organism	Notechis scutatus scutatus (Mainland tiger snake) (Common tiger snake)
Taxonomic identifier	70142 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Lepidosauria › Squamata › Scleroglossa › Serpentes › Colubroidea › Elapidae › Acanthophiinae › Notechis

Protein attributes

Sequence length	98 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Act as a toxin in venom by activating thrombin. It is a procoagulant protein functionally similar to blood coagulation factor Xa.
Catalytic activity	Selective cleavage of Arg-|-Thr and then Arg-|-Ile bonds in prothrombin to form thrombin.
Subunit structure	The two chains are formed from a single-chain precursor and are held together by 1 or more disulfide bonds.
Subcellular location	Secreted.
Tissue specificity	Expressed by the venom gland.
Post-translational modification	The vitamin K-dependent, enzymatic carboxylation of some glutamate residues allows the modified protein to bind calcium.
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 Gla (gamma-carboxy-glutamate) domain.

Ontologies

Keywords
Biological process	Blood coagulation
Cellular component	Secreted
Ligand	Calcium
Molecular function	Hydrolase Toxin
PTM	Disulfide bond Gamma-carboxyglutamic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	blood coagulation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 40	40	Venom coagulation factor Xa-like protease light chain		PRO_0000027819
	Chain	41 – ›98	›58	Venom coagulation factor Xa-like protease heavy chain		PRO_0000027821
Regions
	Domain	1 – 40	40	Gla
	Region	41 – 98	58	Catalytic By similarity
Sites
	Active site	91	1	Charge relay system By similarity
Amino acid modifications
	Modified residue	6	1	4-carboxyglutamate
	Modified residue	7	1	4-carboxyglutamate
	Modified residue	14	1	4-carboxyglutamate
	Modified residue	16	1	4-carboxyglutamate
	Modified residue	19	1	4-carboxyglutamate
	Modified residue	20	1	4-carboxyglutamate
	Modified residue	25	1	4-carboxyglutamate
	Modified residue	26	1	4-carboxyglutamate
	Modified residue	29	1	4-carboxyglutamate
	Modified residue	32	1	4-carboxyglutamate
	Modified residue	35	1	4-carboxyglutamate
	Disulfide bond	17 ↔ 22		By similarity
	Disulfide bond	47 ↔ 52		By similarity
	Disulfide bond	67 ↔ ?		By similarity
Experimental info
	Non-adjacent residues	40 – 41	2
	Non-adjacent residues	78 – 79	2
	Non-terminal residue	98	1

Sequences

	Sequence		Length	Mass (Da)
	P82807-1 [UniParc]. Last modified March 1, 2001. Version 1. Checksum: 6FD398A3608DCFF3	FASTA	98	11,273
	10 20 30 40 50 60 SNSLFEEIRP GNIERECIEE KCSKEEAREV FEDNEKTXXF IVNGMDCKLG ECPWQAVLIN 70 80 90 EKGEVFCGGT ILSPXHVLVH QNFDRVAYDY DIAIIRMK

References

[1]

"Identification and purification of vitamin K-dependent proteins and peptides with monoclonal antibodies specific for gamma-carboxyglutamyl (Gla) residues." Brown M.A., Stenberg L.M., Persson U., Stenflo J. J. Biol. Chem. 275:19795-19802(2000) [PubMed: 10779512] [Abstract] Cited for: PROTEIN SEQUENCE, CHARACTERIZATION. Tissue: Venom.

Cross-references

3D structure databases
HSSP	HSSP built from PDB template 1CFH based on UniProtKB P00740.
ModBase	Search...
Protein family/group databases
MEROPS	S01.425.
Phylogenomic databases
HOVERGEN	P82807.
Enzyme and pathway databases
BRENDA	3.4.21.6. 292759.
Family and domain databases
InterPro	IPR017857. Coagulation_fac_subset_Gla. IPR002383. Coagulation_factor_Gla. IPR000294. GLA_domain. IPR001254. Peptidase_S1_S6. [Graphical view]
Gene3D	G3DSA:4.10.740.10. Coagulation_factor_Gla. 1 hit.
Pfam	PF00594. Gla. 1 hit. PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00001. GLABLOOD.
SMART	SM00069. GLA. 1 hit. [Graphical view]
PROSITE	PS00011. GLA_1. False negative. PS50998. GLA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FA10V_NOTSC

Accession

Primary (citable) accession number: P82807

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	March 1, 2001
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Tox-Prot (Toxin Annotation Project)