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Protein

Partner of Y14 and mago

Gene

Pym

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulator of the exon junction complex (EJC), a multiprotein complex that associates immediately upstream of the exon-exon junction on mRNAs and serves as a positional landmark for the intron exon structure of genes and directs post-transcriptional processes in the cytoplasm such as mRNA export, nonsense-mediated mRNA decay (NMD) or translation. Acts as an EJC disassembly factor by disrupting mature EJC from spliced mRNAs. Required for normal localization of osk mRNA to the posterior pole of the developing oocyte. Does not interact with the small ribosomal unit or components of the translation initiation complex. May not function in cap-dependent translation regulation.1 Publication

GO - Molecular functioni

  • exon-exon junction complex binding Source: FlyBase
  • RNA binding Source: FlyBase

GO - Biological processi

  • exon-exon junction complex disassembly Source: FlyBase
  • nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: UniProtKB
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Partner of Y14 and magoImported
Short name:
DmPYM
Alternative name(s):
Protein within the bgcn gene intron
Gene namesi
Name:PymImported
Synonyms:wibgImported
ORF Names:CG30176Imported
OrganismiDrosophila melanogaster (Fruit fly)Imported
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0034918. Pym.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • exon-exon junction complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Viable and fertile. No visible phenotype.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000659721 – 207Partner of Y14 and magoAdd BLAST207

Proteomic databases

PaxDbiP82804.
PRIDEiP82804.

Expressioni

Tissue specificityi

Expression detected in the ovary. In the oocyte expressed in the germarium, nurse cell and follicle cell.1 Publication

Gene expression databases

BgeeiFBgn0034918.
GenevisibleiP82804. DM.

Interactioni

Subunit structurei

Interacts (via N-terminus) with mago and tsu/RBM8A; the interaction is direct.3 Publications

GO - Molecular functioni

  • exon-exon junction complex binding Source: FlyBase

Protein-protein interaction databases

BioGridi63373. 2 interactors.
IntActiP82804. 3 interactors.
STRINGi7227.FBpp0072142.

Structurei

Secondary structure

1207
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 7Combined sources3
Beta strandi10 – 13Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RK8X-ray1.90C1-58[»]
ProteinModelPortaliP82804.
SMRiP82804.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82804.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili64 – 91Sequence analysisAdd BLAST28
Coiled coili152 – 184Sequence analysisAdd BLAST33

Sequence similaritiesi

Belongs to the pym family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4325. Eukaryota.
ENOG4111KHQ. LUCA.
GeneTreeiENSGT00730000111107.
InParanoidiP82804.
KOiK14294.
OMAiMTTYATD.
OrthoDBiEOG091G0XQ3.
PhylomeDBiP82804.

Family and domain databases

InterProiIPR015362. WIBG_fam.
[Graphical view]
PfamiPF09282. Mago-bind. 1 hit.
[Graphical view]
SMARTiSM01273. Mago-bind. 1 hit.
[Graphical view]
SUPFAMiSSF101931. SSF101931. 1 hit.

Sequencei

Sequence statusi: Complete.

P82804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTYLQSSEG KFIPATKRPD GTWRKARRVK DGYVPQEEVP LYESKGKQFV
60 70 80 90 100
AQRQAGVPPG MCPLLAAESK KEREKQERTR AKKQEKESGR QPKAPAPGVL
110 120 130 140 150
VMPPSTCPPP KVSQQQQQQQ QQPSGSRDIN SISKTLEDTL KLDAAQEVVD
160 170 180 190 200
PAKQLKKLRK KIREIEQIES RIQAGEQKKL DKDQLDKVKK KSEILRQIKD

LESTPRS
Length:207
Mass (Da):23,450
Last modified:March 1, 2001 - v1
Checksum:iF7E6B7C98A2961DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF293388 mRNA. Translation: AAG00610.1.
AJ459405 mRNA. Translation: CAD30676.1.
AE013599 Genomic DNA. Translation: AAM68273.1.
AY070957 mRNA. Translation: AAL48579.1.
AY071704 mRNA. Translation: AAL49326.1.
RefSeqiNP_726372.1. NM_166627.3.
UniGeneiDm.12746.

Genome annotation databases

EnsemblMetazoaiFBtr0072233; FBpp0072142; FBgn0034918.
GeneIDi37780.
KEGGidme:Dmel_CG30176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF293388 mRNA. Translation: AAG00610.1.
AJ459405 mRNA. Translation: CAD30676.1.
AE013599 Genomic DNA. Translation: AAM68273.1.
AY070957 mRNA. Translation: AAL48579.1.
AY071704 mRNA. Translation: AAL49326.1.
RefSeqiNP_726372.1. NM_166627.3.
UniGeneiDm.12746.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1RK8X-ray1.90C1-58[»]
ProteinModelPortaliP82804.
SMRiP82804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63373. 2 interactors.
IntActiP82804. 3 interactors.
STRINGi7227.FBpp0072142.

Proteomic databases

PaxDbiP82804.
PRIDEiP82804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072233; FBpp0072142; FBgn0034918.
GeneIDi37780.
KEGGidme:Dmel_CG30176.

Organism-specific databases

CTDi37780.
FlyBaseiFBgn0034918. Pym.

Phylogenomic databases

eggNOGiKOG4325. Eukaryota.
ENOG4111KHQ. LUCA.
GeneTreeiENSGT00730000111107.
InParanoidiP82804.
KOiK14294.
OMAiMTTYATD.
OrthoDBiEOG091G0XQ3.
PhylomeDBiP82804.

Miscellaneous databases

EvolutionaryTraceiP82804.
GenomeRNAii37780.
PROiP82804.

Gene expression databases

BgeeiFBgn0034918.
GenevisibleiP82804. DM.

Family and domain databases

InterProiIPR015362. WIBG_fam.
[Graphical view]
PfamiPF09282. Mago-bind. 1 hit.
[Graphical view]
SMARTiSM01273. Mago-bind. 1 hit.
[Graphical view]
SUPFAMiSSF101931. SSF101931. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPYM_DROME
AccessioniPrimary (citable) accession number: P82804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: March 1, 2001
Last modified: November 30, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.