P82789 (DF181_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Defensin-like protein 181

Alternative name(s):
Low-molecular-weight cysteine-rich protein 80
Short name=Protein LCR80
Plant defensin 3.1

Gene names

Name:	PDF3.1
Synonyms:	LCR80
Ordered Locus Names:	At5g38330
ORF Names:	MSI17.50

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	122 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Confers broad-spectrum resistance to pathogens.
Subcellular location	Secreted By similarity.
Sequence similarities	Belongs to the DEFL family.
Caution	Contains 8 disulfide bonds instead of the 4 disulfide bonds, which are conserved features of the family.
Sequence caution	The sequence ABK27946.1 differs from that shown. Reason: Erroneous termination at position 123. Translated as stop.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Domain	Signal
Molecular function	Antimicrobial Fungicide
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	defense response Inferred from sequence or structural similarity PubMed 18674922. Source: TAIR defense response to fungus Inferred from electronic annotation. Source: UniProtKB-KW killing of cells of other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 122	96	Defensin-like protein 181		PRO_0000017304
Amino acid modifications
	Disulfide bond	29 ↔ 70		By similarity
	Disulfide bond	36 ↔ 55		By similarity
	Disulfide bond	39 ↔ 64		By similarity
	Disulfide bond	43 ↔ 66		By similarity
	Disulfide bond	76 ↔ 122		By similarity
	Disulfide bond	87 ↔ 107		By similarity
	Disulfide bond	92 ↔ 116		By similarity
	Disulfide bond	96 ↔ 118		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P82789 [UniParc]. Last modified January 4, 2005. Version 2. Checksum: 317369451138883B	FASTA	122	13,122
	10 20 30 40 50 60 MERIPSLASL VSLLIIFATV VNQTRASICN DRLGLCDGCD QRCKAKHGPS CESKCDGPVG 70 80 90 100 110 120 MLLCTCTYEC GPTKLCNGGL GNCGESCNEQ CCDRNCAQRY NGGHGYCNTL DDFSLCLCKY PC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones." Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S. DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Simultaneous high-throughput recombinational cloning of open reading frames in closed and open configurations." Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P. Plant Biotechnol. J. 4:317-324(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Small cysteine-rich peptides resembling antimicrobial peptides have been under-predicted in plants." Silverstein K.A.T., Moskal W.A. Jr., Wu H.C., Underwood B.A., Graham M.A., Town C.D., VandenBosch K.A. Plant J. 51:262-280(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Two large Arabidopsis thaliana gene families are homologous to the Brassica gene superfamily that encodes pollen coat proteins and the male component of the self-incompatibility response." Vanoosthuyse V., Miege C., Dumas C., Cock J.M. Plant Mol. Biol. 46:17-34(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Plant defensins." Thomma B.P.H.J., Cammue B.P., Thevissen K. Planta 216:193-202(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[7]	"Genome organization of more than 300 defensin-like genes in Arabidopsis." Silverstein K.A.T., Graham M.A., Paape T.D., VandenBosch K.A. Plant Physiol. 138:600-610(2005) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011481 Genomic DNA. No translation available. CP002688 Genomic DNA. Translation: AED94298.1. DQ447011 mRNA. Translation: ABE66199.1. DQ912235 mRNA. Translation: ABK27946.1. Sequence problems. EF182827 mRNA. No translation available.
IPI	IPI00522079.
RefSeq	NP_198649.2. NM_123194.2.
UniGene	At.55216.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G38330.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G38330.1; AT5G38330.1; AT5G38330.
GeneID	833815.
KEGG	ath:AT5G38330.
Organism-specific databases
GeneFarm	4628. 429.
TAIR	At5g38330.
Phylogenomic databases
eggNOG	NOG285962.
HOGENOM	HOG000091148.
InParanoid	P82789.
PhylomeDB	P82789.
ProtClustDB	CLSN2689682.
Gene expression databases
Genevestigator	P82789.
Family and domain databases
InterPro	IPR003614. Scorpion_toxin-like. [Graphical view]
SMART	SM00505. Knot1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DF181_ARATH

Accession

Primary (citable) accession number: P82789
Secondary accession number(s): A0MJT6, Q1PDQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	January 4, 2005
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program