P82678 (ALLC_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Allantoicase EC=3.5.3.4 Alternative name(s): Allantoate amidinohydrolase
Organism	Chlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier	3055 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	9 AA.
Sequence status	Fragment.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the degradation of allantoate to (-)-ureidoglycolate and (+)-ureidoglycolate to glyoxylate.
Catalytic activity	Allantoate + H2O = (S)-ureidoglycolate + urea.
Pathway	Nitrogen metabolism; (S)-allantoin degradation; (S)-ureidoglycolate from allantoate (aminidohydrolase route): step 1/1.
Subunit structure	Homohexamer.
Sequence similarities	Belongs to the allantoicase family.
Biophysicochemical properties	Kinetic parameters: Vmax of the reaction with allantoate as substrate is nine times higher than that with ureidoglycolate. KM=2.0 mM for allantoate KM=0.7 mM for ureidoglycolate pH dependence: Optimum pH is 6.5 with allantoate as substrate, and 8 with ureidoglycolate as substrate. Temperature dependence: Optimum temperature is 60 degrees Celsius.

Ontologies

Keywords
Biological process	Purine metabolism
Molecular function	Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	allantoicase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›9	›9	Allantoicase		PRO_0000205915
Experimental info
	Sequence uncertainty	5	1	Q or Y
	Non-terminal residue	9	1

Sequences

	Sequence		Length	Mass (Da)
	P82678 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D934ADD9D6D871F2	FASTA	9	943
	AVHGQFATL

References

[1]	"Allantoate amidinohydrolase (Allantoicase) from Chlamydomonas reinhardtii: its purification and catalytic and molecular characterization." Piedras P., Munoz A., Aguilar M., Pineda M. Arch. Biochem. Biophys. 378:340-348(2000) [PubMed: 10860551] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 6145C.

Cross-references

3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13513.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

ALLC_CHLRE

Accession

Primary (citable) accession number: P82678

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	October 1, 2000
Last modified:	May 31, 2011
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program