P82673 (RT35_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
28S ribosomal protein S35, mitochondrial
Short name=MRP-S35
Short name=S35mt

Alternative name(s):
28S ribosomal protein S28, mitochondrial
Short name=MRP-S28
Short name=S28mt

Gene names

Name:	MRPS35
Synonyms:	MRPS28
ORF Names:	HDCMD11P, MDS023, PSEC0213

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	323 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial ribosome small subunit (28S) which comprises a 12S rRNA and about 30 distinct proteins By similarity. Ref.2 UniProtKB Q2YDF6
Subcellular location	Mitochondrion By similarity Ref.2 UniProtKB Q2YDF6.
Sequence caution	The sequence AAG14958.1 differs from that shown. Reason: Frameshift at position 97. The sequence BAC11579.1 differs from that shown. Reason: Frameshift at position 176.

Ontologies

Keywords
Cellular component	Mitochondrion
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Coiled coil Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA damage response, detection of DNA damage Non-traceable author statement PubMed 15300802. Source: UniProtKB peptide biosynthetic process Inferred by curator PubMed 11279123. Source: UniProtKB
Cellular_component	mitochondrial small ribosomal subunit Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P82673-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P82673-2)

The sequence of this isoform differs from the canonical sequence as follows:
     176-211: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 323		28S ribosomal protein S35, mitochondrial		PRO_0000046055
Regions
	Coiled coil	257 – 321	65	Potential
Natural variations
	Alternative sequence	176 – 211	36	Missing in isoform 2. Ref.2 UniProtKB Q2YDF6		VSP_028424
	Natural variant	6	1	L → I. Corresponds to variant rs35475802 [ dbSNP | Ensembl ].		VAR_052051

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: B29F819E914F2B49	FASTA	323	36,844
	10 20 30 40 50 60 MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM 70 80 90 100 110 120 AVDQDWPSVY PVAAPFKPSA VPLPVRMGYP VKKGVPMAKE GNLELLKIPN FLHLTPVAIK 130 140 150 160 170 180 KHCEALKDFC TEWPAALDSD EKCEKHFPIE IDSTDYVSSG PSVRNPRARV VVLRVKLSSL 190 200 210 220 230 240 NLDDHAKKKL IKLVGERYCK TTDVLTIKTD RCPLRRQNYD YAVYLLTVLY HESWNTEEWE 250 260 270 280 290 300 KSKTEADMEE YIWENSSSER NILETLLQMK AAEKNMEINK EELLGTKEIE EYKKSVVSLK 310 320 NEEENENSIS QYKESVKRLL NVT
	Isoform 2 [UniParc]. Checksum: 830F28D81CC6B473	FASTA	287	32,717
	10 20 30 40 50 60 MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM 70 80 90 100 110 120 AVDQDWPSVY PVAAPFKPSA VPLPVRMGYP VKKGVPMAKE GNLELLKIPN FLHLTPVAIK 130 140 150 160 170 180 KHCEALKDFC TEWPAALDSD EKCEKHFPIE IDSTDYVSSG PSVRNPRARV VVLRVCPLRR 190 200 210 220 230 240 QNYDYAVYLL TVLYHESWNT EEWEKSKTEA DMEEYIWENS SSERNILETL LQMKAAEKNM 250 260 270 280 EINKEELLGT KEIEEYKKSV VSLKNEEENE NSISQYKESV KRLLNVT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel genes expressed in hematopoietic stem/progenitor cells from myelodysplastic syndrome patients." Huang C., Qian B., Tu Y., Gu W., Wang Y., Han Z., Chen Z. Submitted (SEP-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Hematopoietic stem cell.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Teratocarcinoma.
[3]	"Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries." Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J., Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S., Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y. , Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S., Isogai T. DNA Res. 12:117-126(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Embryo.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Colon, Lung and Pancreas.
[5]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 47-323 (ISOFORM 1). Tissue: Melanoma.
[6]	"A novel gene from human dendritic cells." Zhao Z., Huang X., Li N., Zhu X., Cao X. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 180-323 (ISOFORM 1). Tissue: Dendritic cell.
[7]	"Identification of four proteins from the small subunit of the mammalian mitochondrial ribosome using a proteomics approach." Koc E.C., Burkhart W., Blackburn K., Koc H., Moseley A., Spremulli L.L. Protein Sci. 10:471-481(2001) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[8]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF182422 mRNA. Translation: AAG14958.1. Frameshift. AK075378 mRNA. Translation: BAC11579.1. Frameshift. AK075515 mRNA. Translation: BAC11664.1. BC015862 mRNA. Translation: AAH15862.2. BC017086 mRNA. Translation: AAH17086.1. BC028346 mRNA. Translation: AAH28346.2. BC063515 mRNA. Translation: AAH63515.1. BC109372 mRNA. Translation: AAI09373.1. AL512733 mRNA. Translation: CAC21665.1. AF068296 mRNA. Translation: AAF65185.1.
IPI	IPI00073779. IPI00168292.
RefSeq	NP_001177793.1. NM_001190864.1. NP_068593.2. NM_021821.3.
UniGene	Hs.714076.
3D structure databases
ProteinModelPortal	P82673.
ModBase	Search...
Protein-protein interaction databases
IntAct	P82673. 4 interactions.
PTM databases
PhosphoSite	P82673.
Polymorphism databases
DMDM	74708095.
Proteomic databases
PaxDb	P82673.
PRIDE	P82673.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000081029; ENSP00000081029; ENSG00000061794. ENST00000538315; ENSP00000445390; ENSG00000061794.
GeneID	60488.
KEGG	hsa:60488.
UCSC	uc001rih.3. human.
Organism-specific databases
CTD	60488.
GeneCards	GC12P027863.
HGNC	HGNC:16635. MRPS35.
HPA	HPA038513.
MIM	611995. gene.
neXtProt	NX_P82673.
PharmGKB	PA31022.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG267668.
HOVERGEN	HBG082941.
InParanoid	P82673.
OMA	WEKNKTE.
OrthoDB	EOG4W0XDM.
PhylomeDB	P82673.
Gene expression databases
ArrayExpress	P82673.
Bgee	P82673.
CleanEx	HS_MRPS28. HS_MRPS35.
Genevestigator	P82673.
Family and domain databases
InterPro	IPR019349. Ribosomal_S24/S35_mit. [Graphical view]
Pfam	PF10213. MRP-S28. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	60488.
NextBio	65371.
SOURCE	Search...

Entry information

Entry name

RT35_HUMAN

Accession

Primary (citable) accession number: P82673
Secondary accession number(s): Q32LZ1 , Q6P4C6, Q7L1M6, Q8NBP4, Q96AI0, Q9H044, Q9HC14, Q9P1R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.