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Protein

5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Gene

MET6

Organism
Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.

Catalytic activityi

5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.

Cofactori

Zn2+By similarity

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-methionine from L-homocysteine (MetE route).
Proteins known to be involved in this subpathway in this organism are:
  1. 5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (MET6)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-methionine from L-homocysteine (MetE route), the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi657ZincBy similarity1
Metal bindingi659ZincBy similarity1
Metal bindingi739ZincBy similarity1

GO - Molecular functioni

  • 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Source: CGD
  • zinc ion binding Source: CGD

GO - Biological processi

  • cellular response to heat Source: CGD
  • induction by symbiont of host defense response Source: CGD
  • methionine biosynthetic process Source: CGD
  • methionine metabolic process Source: CGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.

Names & Taxonomyi

Protein namesi
Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase (EC:2.1.1.14)
Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme
Gene namesi
Name:MET6
ORF Names:CaO19.10083, CaO19.2551
OrganismiCandida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)
Taxonomic identifieri237561 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida
Proteomesi
  • UP000000559 Componenti: Unassembled WGS sequence

Organism-specific databases

CGDiCAL0000186137. MET6.

Subcellular locationi

GO - Cellular componenti

  • biofilm matrix Source: CGD
  • cell surface Source: CGD
  • fungal-type cell wall Source: CGD
  • hyphal cell wall Source: CGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000987022 – 7675-methyltetrahydropteroyltriglutamate--homocysteine methyltransferaseAdd BLAST766

Proteomic databases

PRIDEiP82610.

2D gel databases

COMPLUYEAST-2DPAGEP82610.

Structurei

Secondary structure

1767
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Helixi17 – 26Combined sources10
Beta strandi28 – 30Combined sources3
Helixi32 – 53Combined sources22
Beta strandi59 – 61Combined sources3
Helixi68 – 75Combined sources8
Helixi81 – 84Combined sources4
Helixi90 – 99Combined sources10
Beta strandi101 – 103Combined sources3
Beta strandi107 – 109Combined sources3
Beta strandi112 – 114Combined sources3
Beta strandi119 – 121Combined sources3
Beta strandi128 – 130Combined sources3
Helixi144 – 146Combined sources3
Helixi149 – 159Combined sources11
Beta strandi165 – 169Combined sources5
Helixi171 – 176Combined sources6
Beta strandi178 – 180Combined sources3
Helixi182 – 184Combined sources3
Helixi189 – 192Combined sources4
Helixi193 – 210Combined sources18
Beta strandi214 – 218Combined sources5
Helixi220 – 223Combined sources4
Helixi228 – 231Combined sources4
Helixi233 – 241Combined sources9
Beta strandi248 – 252Combined sources5
Helixi259 – 261Combined sources3
Helixi262 – 265Combined sources4
Beta strandi271 – 276Combined sources6
Turni277 – 279Combined sources3
Helixi281 – 283Combined sources3
Helixi284 – 289Combined sources6
Beta strandi296 – 302Combined sources7
Helixi312 – 326Combined sources15
Helixi328 – 330Combined sources3
Beta strandi331 – 337Combined sources7
Helixi339 – 341Combined sources3
Helixi346 – 348Combined sources3
Beta strandi350 – 352Combined sources3
Helixi354 – 357Combined sources4
Helixi363 – 377Combined sources15
Helixi383 – 398Combined sources16
Turni400 – 402Combined sources3
Helixi405 – 412Combined sources8
Helixi417 – 419Combined sources3
Helixi425 – 436Combined sources12
Helixi453 – 463Combined sources11
Helixi469 – 489Combined sources21
Beta strandi493 – 495Combined sources3
Helixi507 – 510Combined sources4
Beta strandi513 – 517Combined sources5
Beta strandi524 – 527Combined sources4
Beta strandi530 – 532Combined sources3
Beta strandi536 – 542Combined sources7
Helixi549 – 557Combined sources9
Beta strandi563 – 568Combined sources6
Helixi570 – 575Combined sources6
Beta strandi581 – 583Combined sources3
Helixi585 – 605Combined sources21
Beta strandi610 – 614Combined sources5
Helixi618 – 621Combined sources4
Beta strandi624 – 627Combined sources4
Helixi628 – 645Combined sources18
Beta strandi646 – 648Combined sources3
Beta strandi652 – 658Combined sources7
Beta strandi660 – 662Combined sources3
Helixi665 – 671Combined sources7
Beta strandi674 – 678Combined sources5
Beta strandi682 – 684Combined sources3
Helixi686 – 690Combined sources5
Turni691 – 694Combined sources4
Beta strandi697 – 701Combined sources5
Beta strandi706 – 708Combined sources3
Helixi714 – 725Combined sources12
Helixi730 – 732Combined sources3
Beta strandi733 – 735Combined sources3
Turni740 – 743Combined sources4
Helixi746 – 766Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PPCX-ray2.20A/B1-767[»]
3PPFX-ray2.30A1-767[»]
3PPGX-ray1.98A1-767[»]
3PPHX-ray2.80A/B1-767[»]
4L5ZX-ray2.18A1-767[»]
4L61X-ray2.13A1-767[»]
4L64X-ray2.18A1-767[»]
4L65X-ray2.31A1-767[»]
4L6HX-ray1.75A1-767[»]
4L6OX-ray1.88A1-767[»]
4QQUX-ray2.98A1-767[»]
ProteinModelPortaliP82610.
SMRiP82610.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP82610.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

InParanoidiP82610.
KOiK00549.
OrthoDBiEOG092C0LN1.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82610-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQSSVLGFP RIGGQRELKK ITEAYWSGKA TVEELLAKGK ELREHNWKLQ
60 70 80 90 100
QKAGVDIIPS NDFSYYDQVL DLSLLFNAIP ERYTKFDLAP IDVLFAMGRG
110 120 130 140 150
LQKKATETQA AVDVTALEMV KWFDSNYHYV RPTFSHSTEF KLNTAAGIKP
160 170 180 190 200
VDEFNEAKAL GVQTRPVILG PVSYLYLGKA DKDSLDLEPI SLLPKILPVY
210 220 230 240 250
KELLQKLKEA GAEQVQIDEP VLVLDLPEAV QSKFKEAYDA LVGADVPELI
260 270 280 290 300
LTTYFGDVRP NLKAIENLPV AGFHFDFVRV PEQLDEVASI LKDGQTLSAG
310 320 330 340 350
VVDGRNIWKT DFAKASAVVQ KAIEKVGKDK VVVATSSSLL HTPVDLESET
360 370 380 390 400
KLDAVIKDWF SFATQKLDEV VVIAKNVSGE DVSKQLEANA ASIKARSESS
410 420 430 440 450
ITNDPKVQER LTTINEALAT RKAAFPERLT EQKAKYNLPL FPTTTIGSFP
460 470 480 490 500
QTKDIRINRN KFAKGQITAE EYEAFINKEI ETVVRFQEEI GLDVLVHGEP
510 520 530 540 550
ERNDMVQYFG EQLNGFAFTT NGWVQSYGSR YVRPPIIVGD VSRPKAMTVK
560 570 580 590 600
ESVYAQSITS KPMKGMLTGP VTILRWSFPR DDVSGKIQAL QLGLALRDEV
610 620 630 640 650
NDLEGAGITV IQVDEPAIRE GLPLRAGKER SDYLNWAAQS FRVATSGVEN
660 670 680 690 700
STQIHSHFCY SDLDPNHIKA LDADVVSIEF SKKDDPNYIQ EFSEYPNHIG
710 720 730 740 750
LGLFDIHSPR IPSKQEFVSR IEEILKVYPA SKFWVNPDCG LKTRGWPEVK
760
ESLTNMVEAA KEFRAKY
Length:767
Mass (Da):85,702
Last modified:March 3, 2009 - v2
Checksum:i21BE228C99259471
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5S → D AA sequence (PubMed:11681208).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000043 Genomic DNA. Translation: EAK99386.1.
AACQ01000044 Genomic DNA. Translation: EAK99287.1.
RefSeqiXP_718219.1. XM_713126.1.
XP_718315.1. XM_713222.1.

Genome annotation databases

EnsemblFungiiEAK99287; EAK99287; CaO19.2551.
EAK99386; EAK99386; CaO19.10083.
GeneIDi3640000.
3640084.
KEGGical:CaO19.10083.
cal:CaO19.2551.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACQ01000043 Genomic DNA. Translation: EAK99386.1.
AACQ01000044 Genomic DNA. Translation: EAK99287.1.
RefSeqiXP_718219.1. XM_713126.1.
XP_718315.1. XM_713222.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PPCX-ray2.20A/B1-767[»]
3PPFX-ray2.30A1-767[»]
3PPGX-ray1.98A1-767[»]
3PPHX-ray2.80A/B1-767[»]
4L5ZX-ray2.18A1-767[»]
4L61X-ray2.13A1-767[»]
4L64X-ray2.18A1-767[»]
4L65X-ray2.31A1-767[»]
4L6HX-ray1.75A1-767[»]
4L6OX-ray1.88A1-767[»]
4QQUX-ray2.98A1-767[»]
ProteinModelPortaliP82610.
SMRiP82610.
ModBaseiSearch...
MobiDBiSearch...

2D gel databases

COMPLUYEAST-2DPAGEP82610.

Proteomic databases

PRIDEiP82610.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiEAK99287; EAK99287; CaO19.2551.
EAK99386; EAK99386; CaO19.10083.
GeneIDi3640000.
3640084.
KEGGical:CaO19.10083.
cal:CaO19.2551.

Organism-specific databases

CGDiCAL0000186137. MET6.

Phylogenomic databases

InParanoidiP82610.
KOiK00549.
OrthoDBiEOG092C0LN1.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00082.

Miscellaneous databases

EvolutionaryTraceiP82610.

Family and domain databases

HAMAPiMF_00172. Meth_synth. 1 hit.
InterProiIPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Met_Synth_C/arc.
[Graphical view]
PfamiPF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]
PIRSFiPIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMsiTIGR01371. met_syn_B12ind. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMETE_CANAL
AccessioniPrimary (citable) accession number: P82610
Secondary accession number(s): Q5A9B4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: March 3, 2009
Last modified: November 30, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Candida albicans
    Candida albicans: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.