P82610 (METE_CANAL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	MET6
ORF Names:	CaO19.2551, CaO19.10083

Organism

Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast)

Taxonomic identifier

237561 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida

Protein attributes

Sequence length	767 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.
Cofactor	Zinc By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	cellular response to heat Inferred from expression pattern. Source: CGD induction by symbiont of host defense response Inferred from direct assay Ref.2. Source: CGD methionine biosynthetic process Inferred from mutant phenotype. Source: CGD
Cellular component	cell surface Inferred from direct assay. Source: CGD hyphal cell wall Inferred from direct assay. Source: CGD soluble fraction Inferred from direct assay. Source: CGD
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from direct assay. Source: CGD zinc ion binding Inferred from direct assay. Source: CGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.2
	Chain	2 – 767	766	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase		PRO_0000098702
Sites
	Metal binding	657	1	Zinc By similarity
	Metal binding	659	1	Zinc By similarity
	Metal binding	739	1	Zinc By similarity
Experimental info
	Sequence conflict	5	1	S → D AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P82610 [UniParc]. Last modified March 3, 2009. Version 2. Checksum: 21BE228C99259471	FASTA	767	85,702
	10 20 30 40 50 60 MVQSSVLGFP RIGGQRELKK ITEAYWSGKA TVEELLAKGK ELREHNWKLQ QKAGVDIIPS 70 80 90 100 110 120 NDFSYYDQVL DLSLLFNAIP ERYTKFDLAP IDVLFAMGRG LQKKATETQA AVDVTALEMV 130 140 150 160 170 180 KWFDSNYHYV RPTFSHSTEF KLNTAAGIKP VDEFNEAKAL GVQTRPVILG PVSYLYLGKA 190 200 210 220 230 240 DKDSLDLEPI SLLPKILPVY KELLQKLKEA GAEQVQIDEP VLVLDLPEAV QSKFKEAYDA 250 260 270 280 290 300 LVGADVPELI LTTYFGDVRP NLKAIENLPV AGFHFDFVRV PEQLDEVASI LKDGQTLSAG 310 320 330 340 350 360 VVDGRNIWKT DFAKASAVVQ KAIEKVGKDK VVVATSSSLL HTPVDLESET KLDAVIKDWF 370 380 390 400 410 420 SFATQKLDEV VVIAKNVSGE DVSKQLEANA ASIKARSESS ITNDPKVQER LTTINEALAT 430 440 450 460 470 480 RKAAFPERLT EQKAKYNLPL FPTTTIGSFP QTKDIRINRN KFAKGQITAE EYEAFINKEI 490 500 510 520 530 540 ETVVRFQEEI GLDVLVHGEP ERNDMVQYFG EQLNGFAFTT NGWVQSYGSR YVRPPIIVGD 550 560 570 580 590 600 VSRPKAMTVK ESVYAQSITS KPMKGMLTGP VTILRWSFPR DDVSGKIQAL QLGLALRDEV 610 620 630 640 650 660 NDLEGAGITV IQVDEPAIRE GLPLRAGKER SDYLNWAAQS FRVATSGVEN STQIHSHFCY 670 680 690 700 710 720 SDLDPNHIKA LDADVVSIEF SKKDDPNYIQ EFSEYPNHIG LGLFDIHSPR IPSKQEFVSR 730 740 750 760 IEEILKVYPA SKFWVNPDCG LKTRGWPEVK ESLTNMVEAA KEFRAKY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The diploid genome sequence of Candida albicans." Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B., Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W., Scherer S. Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004) [PubMed: 15123810] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SC5314 / ATCC MYA-2876.
[2]	"Analysis of the serologic response to systemic Candida albicans infection in a murine model." Pitarch A., Diez-Orejas R., Molero G., Pardo M., Sanchez M., Gil C., Nombela C. Proteomics 1:550-559(2001) [PubMed: 11681208] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-15. Strain: SC5314 / ATCC MYA-2876.
[3]	"Cross-species identification of novel Candida albicans immunogenic proteins by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry." Pardo M., Ward M., Pitarch A., Sanchez M., Nombela C., Blackstock W., Gil C. Electrophoresis 21:2651-2659(2000) [PubMed: 10949142] [Abstract] Cited for: PROTEIN SEQUENCE OF 411-421, MASS SPECTROMETRY. Strain: SC5314 / ATCC MYA-2876.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AACQ01000043 Genomic DNA. Translation: EAK99386.1.
AACQ01000044 Genomic DNA. Translation: EAK99287.1.

RefSeq

XP_718219.1. XM_713126.1.
XP_718315.1. XM_713222.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3PPC	X-ray	2.20	A/B	1-767	[»]
3PPF	X-ray	2.30	A	1-767	[»]
3PPG	X-ray	1.98	A	1-767	[»]
3PPH	X-ray	2.80	A/B	1-767	[»]

ProteinModelPortal

P82610.

ModBase

Search...

Protein-protein interaction databases

STRING

P82610.

2D gel databases

COMPLUYEAST-2DPAGE

P82610.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

3640000.
3640084.

KEGG

cal:CaO19.10083.
cal:CaO19.2551.

Organism-specific databases

CGD

CAL0002475. MET6.

Phylogenomic databases

OMA

NEPKLDK.

PhylomeDB

P82610.

Family and domain databases

InterPro

IPR013215. Cbl-indep_Met_Synth_N.
IPR006276. Cobalamin-indep_Met_synthase.
IPR002629. Methionine_synth.
[Graphical view]

KO

K00549.

Pfam

PF08267. Meth_synt_1. 1 hit.
PF01717. Meth_synt_2. 1 hit.
[Graphical view]

PIRSF

PIRSF000382. MeTrfase_B12_ind. 1 hit.

TIGRFAMs

TIGR01371. Met_syn_B12ind. 1 hit.

ProtoNet

Search...

Entry information

Entry name

METE_CANAL

Accession

Primary (citable) accession number: P82610
Secondary accession number(s): Q5A9B4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	March 3, 2009
Last modified:	January 25, 2012
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program