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Protein

Nitrilase

Gene

nit

Organism
Bacillus sp. (strain OxB-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A nitrile + 2 H2O = a carboxylate + NH3.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei47Proton acceptorPROSITE-ProRule annotation1
Active sitei128Proton donorPROSITE-ProRule annotation1
Active sitei162NucleophilePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrilase (EC:3.5.5.1)
Gene namesi
Name:nit
OrganismiBacillus sp. (strain OxB-1)
Taxonomic identifieri98228 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002040432 – 339NitrilaseAdd BLAST338

Structurei

3D structure databases

ProteinModelPortaliP82605.
SMRiP82605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 277CN hydrolasePROSITE-ProRule annotationAdd BLAST271

Sequence similaritiesi

Family and domain databases

Gene3Di3.60.110.10. 1 hit.
InterProiView protein in InterPro
IPR003010. C-N_Hydrolase.
IPR036526. C-N_Hydrolase_sf.
IPR000132. Nitrilase/CN_hydratase_CS.
PfamiView protein in Pfam
PF00795. CN_hydrolase. 1 hit.
SUPFAMiSSF56317. SSF56317. 1 hit.
PROSITEiView protein in PROSITE
PS50263. CN_HYDROLASE. 1 hit.
PS00920. NITRIL_CHT_1. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNYPKYRVA AVQASPVLLD LDATIDKTCR LVDEAAANGA KVIAFPEAFI
60 70 80 90 100
PGYPWWIWLG NADYGMKYYI QLYKNSVEIP SLAVQKLSSA GTNKVYFCVS
110 120 130 140 150
VTEKDGGSLY LTQLWFDPNG DLIGKHRKLK ATNAEKTIWG DGDGSMMPVF
160 170 180 190 200
ETEFGNLGGL QCWEHFLPLN VAAMASMNEQ VHVASWPIGM PQEGHLFGPE
210 220 230 240 250
QCVTATKYYA ISNQVFCLLS SQIWTEEQRD KICETEEQRN FMKVGHGFSK
260 270 280 290 300
IIAPNGMEIG NKLAHDEEGI TYADIDLEQI IPGKFLIDSA GHYSTPGFLS
310 320 330
LSFDRTEKKP IKHIGESAQE TVTYEEIQYG NKANVKVHS
Length:339
Mass (Da):37,784
Last modified:January 23, 2007 - v2
Checksum:iC47A9601BBCE7275
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB028892 Genomic DNA. Translation: BAA90460.1.

Similar proteinsi

Entry informationi

Entry nameiNRL_BACSX
AccessioniPrimary (citable) accession number: P82605
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 31, 2004
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 48 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families