##gff-version 3
P82597	UniProtKB	Initiator methionine	1	1	.	.	.	Note=Removed;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:10731713;Dbxref=PMID:10731713	
P82597	UniProtKB	Chain	2	250	.	.	.	ID=PRO_0000207072;Note=Thermostable monoacylglycerol lipase	
P82597	UniProtKB	Active site	97	97	.	.	.	Note=Nucleophile	
P82597	UniProtKB	Active site	196	196	.	.	.	Note=Charge relay system	
P82597	UniProtKB	Active site	226	226	.	.	.	Note=Charge relay system	
P82597	UniProtKB	Binding site	29	29	.	.	.	.	
P82597	UniProtKB	Binding site	98	98	.	.	.	.	
P82597	UniProtKB	Site	145	145	.	.	.	Note=Important for substrate specificity	
P82597	UniProtKB	Mutagenesis	145	145	.	.	.	Note=18%25 reduction in hydrolase activity for both 1-lauroylglycerol (1-LG) and 1-oleoylglycerol (1-OG). I->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24014019;Dbxref=PMID:24014019	
P82597	UniProtKB	Mutagenesis	145	145	.	.	.	Note=62%25 and 38%25 reduction in hydrolase activity for 1-oleoylglycerol and 1-lauroylglycerol%2C respectively. I->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24014019;Dbxref=PMID:24014019	
P82597	UniProtKB	Mutagenesis	196	196	.	.	.	Note=Loss of enzyme activity. D->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:24014019;Dbxref=PMID:24014019	
P82597	UniProtKB	Beta strand	14	16	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	19	26	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	33	35	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	37	45	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	49	52	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	58	60	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4KEA	
P82597	UniProtKB	Helix	62	66	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	70	85	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	89	96	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	98	109	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	115	120	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	126	131	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	134	136	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4KEA	
P82597	UniProtKB	Beta strand	140	143	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	160	163	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	164	179	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	181	183	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	188	193	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	197	199	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	203	210	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Beta strand	214	223	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	228	230	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3	
P82597	UniProtKB	Helix	234	248	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3RM3