Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P82594

- IABF_STRCX

UniProt

P82594 - IABF_STRCX

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Extracellular exo-alpha-(1->5)-L-arabinofuranosidase

Gene
N/A
Organism
Streptomyces chartreusis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes only the cleavage of terminal alpha-(1->5) arabinofuranosyl bonds of arabinan present in the arabinofuranosyl polysaccharides or oligosaccharides. It cannot act on other arabinose-containing polysaccharides and arabinoxylo-oligosaccharides.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

pH dependencei

Optimum pH is 7.0. The enzyme is slowly inactivated above pH 9 and below pH 5.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius. The enzyme is inactivated at temperatures above 40 degrees Celsius.1 Publication

Pathwayi

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. arabinan catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

UniPathwayiUPA00667.

Protein family/group databases

CAZyiGH43. Glycoside Hydrolase Family 43.

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular exo-alpha-(1->5)-L-arabinofuranosidase (EC:3.2.1.55)
Short name:
ABF
Alternative name(s):
Alpha-L-AFase
Extracellular arabinan exo-alpha-(1->5)-L-arabinosidase
Short name:
Arabinosidase
OrganismiStreptomyces chartreusis
Taxonomic identifieri1969 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4343Tat-tyPE signal1 PublicationAdd
BLAST
Chaini44 – 328285Extracellular exo-alpha-(1->5)-L-arabinofuranosidasePRO_0000012203Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Structurei

3D structure databases

ProteinModelPortaliP82594.
SMRiP82594. Positions 51-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 43 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.115.10.20. 1 hit.
InterProiIPR016828. Alpha-L-arabinofuranosidase.
IPR006710. Glyco_hydro_43.
IPR023296. Glyco_hydro_beta-prop.
IPR006311. TAT_signal.
[Graphical view]
PANTHERiPTHR22925. PTHR22925. 1 hit.
PfamiPF04616. Glyco_hydro_43. 1 hit.
[Graphical view]
PIRSFiPIRSF025414. Alpha-L-arabinofuranosidase. 1 hit.
SUPFAMiSSF75005. SSF75005. 1 hit.
PROSITEiPS51318. TAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82594-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MCTREAVRMS REHDLPEIPS RRLLLKGAAA AGALTAVPGV AHAAPRPAPY
60 70 80 90 100
ENPLVRQRAD PHIHRHTDGR YYFTATAPEY DRIVLRRSRT LGGLSTAAES
110 120 130 140 150
VIWRAHPTGD MAAHIWAPEL HRIGGKWYVY FAAAPAEDVW RIRIWVLENS
160 170 180 190 200
HPDPFKGTWE EKGQVRTAWE TFSLDATTFT HRGARYLCWA QHEPGADNNT
210 220 230 240 250
GLFLSEMANP WTLTGPQIRL STPEYDWECV GYKVNEGPYA LKRNGRIFLT
260 270 280 290 300
YSASATDHHY CVGMFTADAG GNLMDPGNWS KSPIPVFTGN ETTKQYGPGH
310 320
NCFTVAEDGR SDVLVYHARQ YKEIVGDP
Length:328
Mass (Da):36,774
Last modified:October 1, 2000 - v1
Checksum:iAA7D5C1D2ABF38D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023626 Genomic DNA. Translation: BAA90772.1.
PIRiB59296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB023626 Genomic DNA. Translation: BAA90772.1 .
PIRi B59296.

3D structure databases

ProteinModelPortali P82594.
SMRi P82594. Positions 51-326.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH43. Glycoside Hydrolase Family 43.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00667 .

Family and domain databases

Gene3Di 2.115.10.20. 1 hit.
InterProi IPR016828. Alpha-L-arabinofuranosidase.
IPR006710. Glyco_hydro_43.
IPR023296. Glyco_hydro_beta-prop.
IPR006311. TAT_signal.
[Graphical view ]
PANTHERi PTHR22925. PTHR22925. 1 hit.
Pfami PF04616. Glyco_hydro_43. 1 hit.
[Graphical view ]
PIRSFi PIRSF025414. Alpha-L-arabinofuranosidase. 1 hit.
SUPFAMi SSF75005. SSF75005. 1 hit.
PROSITEi PS51318. TAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization and gene cloning of two alpha-L-arabinofuranosidases from Streptomyces chartreusis GS901."
    Matsuo N., Kaneko S., Kuno A., Kobayashi H., Kusakabe I.
    Biochem. J. 346:9-15(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 44-72, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
    Strain: GS901.

Entry informationi

Entry nameiIABF_STRCX
AccessioniPrimary (citable) accession number: P82594
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3