P82594 (ABF2_STRCX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Alpha-N-arabinofuranosidase 2 EC=3.2.1.55 Alternative name(s): Alpha-N-arabinofuranosidase II Short name=Alpha-N-AFase II Short name=Arabinosidase II
Organism	Streptomyces chartreusis
Taxonomic identifier	1969 [NCBI]
Taxonomic lineage	Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Streptomycineae › Streptomycetaceae › Streptomyces

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Subcellular location	Secreted.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the glycosyl hydrolase 43 family.
Biophysicochemical properties	pH dependence: Optimum pH is 7.0. Temperature dependence: Optimum temperature is 50 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 43	43	Tat-type signal Ref.1
	Chain	44 – 328	285	Alpha-N-arabinofuranosidase 2		PRO_0000012203

Sequences

	Sequence		Length	Mass (Da)
	P82594 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: AA7D5C1D2ABF38D5	FASTA	328	36,774
	10 20 30 40 50 60 MCTREAVRMS REHDLPEIPS RRLLLKGAAA AGALTAVPGV AHAAPRPAPY ENPLVRQRAD 70 80 90 100 110 120 PHIHRHTDGR YYFTATAPEY DRIVLRRSRT LGGLSTAAES VIWRAHPTGD MAAHIWAPEL 130 140 150 160 170 180 HRIGGKWYVY FAAAPAEDVW RIRIWVLENS HPDPFKGTWE EKGQVRTAWE TFSLDATTFT 190 200 210 220 230 240 HRGARYLCWA QHEPGADNNT GLFLSEMANP WTLTGPQIRL STPEYDWECV GYKVNEGPYA 250 260 270 280 290 300 LKRNGRIFLT YSASATDHHY CVGMFTADAG GNLMDPGNWS KSPIPVFTGN ETTKQYGPGH 310 320 NCFTVAEDGR SDVLVYHARQ YKEIVGDP

References

[1]

"Purification, characterization and gene cloning of two alpha-L-arabinofuranosidases from Streptomyces chartreusis GS901." Matsuo N., Kaneko S., Kuno A., Kobayashi H., Kusakabe I. Biochem. J. 346:9-15(2000) [PubMed: 10657233] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 44-72. Strain: GS901.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB023626 Genomic DNA. Translation: BAA90772.1.
PIR	B59296.
3D structure databases
ProteinModelPortal	P82594.
SMR	P82594. Positions 51-326.
ModBase	Search...
Protein family/group databases
CAZy	GH43. Glycoside Hydrolase Family 43.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016828. Alpha-L-arabinofuranosidase. IPR006710. Glyco_hydro_43. IPR023296. Glyco_hydro_43_beta-prop. IPR006311. TAT_signal. [Graphical view]
Gene3D	G3DSA:2.115.10.20. Glyco_hydro_43_beta-prop. 1 hit.
PANTHER	PTHR22925. Glyco_hydro_43. 1 hit.
Pfam	PF04616. Glyco_hydro_43. 1 hit. [Graphical view]
PIRSF	PIRSF025414. Alpha-L-arabinofuranosidase. 1 hit.
SUPFAM	SSF75005. Glyco_hydro_43_beta-prop. 1 hit.
PROSITE	PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABF2_STRCX

Accession

Primary (citable) accession number: P82594

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 17, 2003
Last sequence update:	October 1, 2000
Last modified:	May 31, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program