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P82593

- EABF_STRCX

UniProt

P82593 - EABF_STRCX

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Protein

Extracellular exo-alpha-L-arabinofuranosidase

Gene
N/A
Organism
Streptomyces chartreusis
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Involved in the degradation of arabinan and is a key enzyme in the complete degradation of the plant cell wall. Catalyzes the cleavage of terminal alpha-L-arabinofuranosyl residues of arabinan present in the arabinofuranosyl polysaccharides or oligosaccharides. It cannot act on other arabinose-containing polysaccharides and arabinoxylo-oligosaccharides. It leaves most of the polymer intact, including most of the main-chain residues and the arabinose side chains. It acts preferentially on the linear alpha-(1->2)-linked arabinofuranobiosides and alpha-(1->3)-linked arabinofuranobiosides, and is much less effective on alpha-(1->5)-linked arabinofuranobiosides. It also hydrolyzes the terminal alpha-(1->3)-linked arabinofuranotriosides in preference to the alpha-(1->5)-linked arabinofuranotriosides.1 Publication

Catalytic activityi

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.1 Publication

pH dependencei

Optimum pH is 5.5. The enzyme is slowly inactivated above pH 8.5 and below pH 5.5.1 Publication

Temperature dependencei

Optimum temperature is 55 degrees Celsius. The enzyme is inactivated at temperatures above 45 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei58 – 581Alpha-L-arabinofuranoseBy similarity
Binding sitei247 – 2471Alpha-L-arabinofuranose; via amide nitrogenBy similarity
Active sitei380 – 3801Proton donor/acceptorBy similarity
Sitei607 – 6071Important for substrate recognitionBy similarity

GO - Molecular functioni

  1. alpha-L-arabinofuranosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. L-arabinose metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Protein family/group databases

CAZyiGH51. Glycoside Hydrolase Family 51.

Names & Taxonomyi

Protein namesi
Recommended name:
Extracellular exo-alpha-L-arabinofuranosidase (EC:3.2.1.55)
Short name:
ABF
Alternative name(s):
Alpha-L-AFase
Extracellular arabinan exo-alpha-L-arabinosidase
Short name:
Arabinosidase
OrganismiStreptomyces chartreusis
Taxonomic identifieri1969 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesStreptomycineaeStreptomycetaceaeStreptomyces

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 825796Extracellular exo-alpha-L-arabinofuranosidasePRO_0000012217Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP82593.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 215146CBM-cenCAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni379 – 3802Alpha-L-arabinofuranose bindingBy similarity

Sequence similaritiesi

Belongs to the glycosyl hydrolase 51 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
InterProiIPR010720. Alpha-L-AF_C.
IPR003305. CenC_carb-bd.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF06964. Alpha-L-AF_C. 1 hit.
PF02018. CBM_4_9. 1 hit.
[Graphical view]
SMARTiSM00813. Alpha-L-AF_C. 1 hit.
[Graphical view]
SUPFAMiSSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P82593-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRIRWRYGT AATALLVAAG LVPTATAHAE DVTDYSITVD PAAKGAAIDD
60 70 80 90 100
TMYGVFFEDI NRAADGGLYA ELVQNRSFEY STDDNRSYTP LTSWIVDGTG
110 120 130 140 150
EVVNDAGRLN ERNRNYLSLG AGSSVTNAGY NTGIRVEQGK RYDFSVWARA
160 170 180 190 200
GSASTLTVAL KDAAGTLATA RQVAVEGGWA KYRATFTATR TSNRGRLAVA
210 220 230 240 250
ANDAAALDMV SLFPRDTYRN QQNGLRKDLA EKIAALHPGF VRFPGGCLVN
260 270 280 290 300
TGSMEDYSAA SGWQRKRSYQ WKDTVGPVEE RATNANFWGY NQSYGLGYYE
310 320 330 340 350
YFRFSEDIGA MPLPVVPALV TGCGQNKAVD DEALLKRHIQ DTLDLIEFAN
360 370 380 390 400
GPATSKWGKV RAEMGHPRPF RLTHLEVGNE ENLPDEFFDR FKQFRAAIEA
410 420 430 440 450
EYPDITVVSN SGPDDAGTTF DTAWKLNREA NVEMVDEHYY NSPNWFLQNN
460 470 480 490 500
DRYDSYDRGG PKVFLGEYAS QGNAWKNGLS EAAFMTGLER NADVVKLASY
510 520 530 540 550
APLLANEDYV QWRPDLVWFN NRASWNSANY EVQKLFMNNV GDRVVPSKAT
560 570 580 590 600
TTPDVSGPIT GAVGLSTWAT GTAYDDVKVT AADGATLLSD DFSGDASKWT
610 620 630 640 650
HTGAGSWSVQ DGQYVQTDAA AENTMVQAGD PSWHDYDLHV KATKKSGKEG
660 670 680 690 700
FLVAFGVKDT GNYYWWNLGG WNNTQSAVEQ AVDGGKGTLL TKAGSIETGR
710 720 730 740 750
AYDIDVKVRG RQVTLYLDGQ EWGGFTDDKP AEPFRQVVTK DARTGDLIVK
760 770 780 790 800
VVNAQPAEAR TAIDLGGARV ASTARVTTLA ADQDAVNTET DAPVTPATST
810 820
FSGVTDRFTY TFPANSVTFL RLKQR
Length:825
Mass (Da):90,184
Last modified:October 1, 2000 - v1
Checksum:i9FF4EE99BE0C696E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023625 Genomic DNA. Translation: BAA90771.1.
PIRiA59296.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB023625 Genomic DNA. Translation: BAA90771.1 .
PIRi A59296.

3D structure databases

ProteinModelPortali P82593.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH51. Glycoside Hydrolase Family 51.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 2.60.120.260. 1 hit.
InterProi IPR010720. Alpha-L-AF_C.
IPR003305. CenC_carb-bd.
IPR013320. ConA-like_dom.
IPR008979. Galactose-bd-like.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF06964. Alpha-L-AF_C. 1 hit.
PF02018. CBM_4_9. 1 hit.
[Graphical view ]
SMARTi SM00813. Alpha-L-AF_C. 1 hit.
[Graphical view ]
SUPFAMi SSF49785. SSF49785. 1 hit.
SSF49899. SSF49899. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Purification, characterization and gene cloning of two alpha-L-arabinofuranosidases from Streptomyces chartreusis GS901."
    Matsuo N., Kaneko S., Kuno A., Kobayashi H., Kusakabe I.
    Biochem. J. 346:9-15(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 30-75, FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBSTRATE SPECIFICITY.
    Strain: GS901.

Entry informationi

Entry nameiEABF_STRCX
AccessioniPrimary (citable) accession number: P82593
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 17, 2003
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3